Posted by admin on Oct 1, 2011 in |
A new, simple, safe, accurate and reproducible spectrophotometric analytical method was developed for the quantitative estimation of gatifloxacin in solid dosage form by mixed hydrotropic agents. The enhancement of solubility of drug gatifloxacin was more than 15 fold in mixed hydrotropic solution (20% N,N dimethyl urea and 20% sodium citrate solution) as compared to solubility in distilled water. Therefore, it was thought worthwhile to solubilize this poorly water soluble drug from fine powder of its tablets by this novel mixed hydrotropic solubilization technique and then carryout its spectrophotometric estimation at 333 nm (20% N,N dimethyl urea and 20% sodium citrate being non-interfering in the estimation). The results of the analysis were validated statistically and by recovery studies & its follows Beer’s law in concentration range of 10-60 mcg/ml. The percent label claims and percent recoveries estimated were close to 100 with low values of standard deviation, percent coefficient of variation and standard error. Thus, the method was accurate providing additional advantage of being cost effective and environment...
Read More
Posted by admin on Oct 1, 2011 in |
Trigonella foenum graecum, (Fenugreek) (Family: Leguminosae) is used as a traditional medicine for anti-inflammatory, antiseptic, antidiabetic and antilipidemic. Hydroalcoholic extract of its seeds were subjected for in vitro antioxidant activity by different methods viz, 1- diphenylpicryl- hydrazyl radical (DPPH), hydroxyl, and ABTS radical cation assay. Total antioxidant capacity, Total phenol and total flavonoid, Total ascorbic acid assay also were estimated. It was observed that free radicals were scavenged by the extract in a dose dependent manner in all the models The content of total phenolics (expressed as mg of gallic acid equivalents/gm) and total flavonoids (expressed as mg of quercetin equivalent/gm) and ascorbic acid were determined along with antioxidant enzymes. The Hydroalcoholic extract of Trigonella foenum graecum, exhibited potent DPPH and ABTS radical scavenging activity with IC50 values of 350µg/ml, and 962.5µg/ml, respectively. The seeds of fenugreek showed significant total antioxidant capacity with IC50 value of 192 µg/ml and hydroxyl radical with IC50 value of 587.5µg/ml, respectively. Based on the results it can be concluded that hydroalcoholic extract of...
Read More
Posted by admin on Oct 1, 2011 in |
In this study, we investigated and compared the total phenolic, alkaloid content and in-vitro antioxidant activity of Leucas aspera collected from four different regions; Tirupathi (Southern zone), Lam (Krishna river region), Jagityala (Northern Telangana) and Hyderabad (Southern Telangana) of Andhra Pradesh, India. Quantitative regional variation was observed in total phenolic content, and alkaloid content in methanolic extracts of Leucas aspera from above four regions of Andhra Pradesh. Concentration dependent antioxidant activity was observed for all these extracts and also observed regional variation for scavenging of Superoxide, Hydroxyl and DPPH radicals. Among the four regions, Leucas aspera from Jagityala region contains more phenolic content (48.06±0.4µg/100 µg), Tirupathi region contains good alkaloid content (58.6±0.1µg/mg) and Hyderabad zone showed better free radical scavenging activity (IC50 value for superoxide radical 156.34µg, Hydroxyl radical 122.34µg and DPPH radical 57.12µg...
Read More
Posted by admin on Oct 1, 2011 in |
Context: The leaves of Nyctanthes arbor-trisitis (Linn.) are used in Traditional System of Medicine for the treatment of stomachic, carminative, intestinal astringent, expectorant, biliousness piles, hair tonic and wound healing. Objective: To evaluate wound healing activity of Nyctanthes arbor-trisitis (Linn.) Material & Methods: Extracts from the dried leaves of Nyctanthes arbor-trisitis (Linn.) were prepared using methanol as solvent in order to investigate the wound healing activity in vivo. Circular excision and linear incision wounds were created on rats. Three groups of rats were prepared viz. control, standard and treated with extract. The methanol extract of Nyctanthes arbor-trisitis (Linn.) was tested for wound healing activity. Results & Discussion: The wounds were monitored and the area of wound was measured on 4, 8, 12, 16 post-wounding days and the mean % wound closure were reported. Epithelization period was calculated as the number of days required for falling of the dead tissue remnants without any residual raw wound. Wound healing rate was measured using formula (Muthusamy et al., 2008). Significant wound healing...
Read More
Posted by admin on Oct 1, 2011 in |
Chonemorpha grandiflora, (Roth) M.R. and S.M. Almeida syn C. fragrans (Moon), Alston (Apocynaceae) has been included in the list of an endangered medicinal plants. It is used in medicinal preparations like kumaryasavam and sudarsanasavum used as tonic3. Entire plant, roots and root bark are used for fever and stomach disorders. The plant is useful in treatment of skin diseases and inflammations. Phytochemical investigations were carried out using in vivo and in vitro plant material of C. grandiflora collected from Dandeli, Uttara Kannada district, Karnataka state. Percentage of total alkaloids was found to be more in stem with bark as compared to the leaves whereas percentage of total alkaloids in callus was found to be more as compared to in vitro plantlets. TLC studies revealed different alkaloid profile in callus as compared to in vitro plantlets. HPLC analysis revealed the presence of camptothecin, an important anticancer compound in the ethanolic extracts of stem with bark of Chonemorpha grandiflora from Thrissur district, Kerala state (Kulkarni et al, 2010). The presence of...
Read More
