Posted by admin on Apr 30, 2023 in |
Background: Our study aims to document the use of ethnomedicinal plants reported by herbalists in treating ailments that are becoming more prevalent. Using an ethnobotanical approach and in-silico studies, will advise further prevalence and give precedence to future pharmacological studies. Methods: The application of the plants was reported by 75 informants. From the 51 reported plants, 20 plants were frequently cited as having high potential anti viral and anti-inflammatory activity and were validated in in-silico approach. PASS was used for compound prediction, and AMT was calculated with the help of ADMET lab 2.0. Results: Out of the 20 plants validated in in-silico, the results of reported compounds revealed that Aeginetia indica could best act as an antiviral agent with Pa (0.939) and Pi (0.003), followed by Bacopa monnieri with Pa (0.880) and Pi (0.002) and Blumea balsamifera with Pa (0.743) and Pi (0.004). Subsequently, the plant which can act as the best anti-inflammatory ailment is Andrographis paniculata with Pa (0.845) and Pi (0.005), followed by Pisum sativum with Pa...
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Posted by admin on Apr 30, 2023 in |
Vigna aconitifolia L. represents a genus in the Fabaceae family and is one of the most drought-resistant legumes, commonly grown in arid and semi-arid regions of India. Therapeutic effects of Vigna aconitifolia L. have been reported in several publications, but there are only a few reports on the biological activities of the Vigna aconitifolia L. proteins. In the present study, antibacterial activities of the Vigna aconitifolia L., peptides have been investigated. Homogenized seed flour was pulverized, and its protein content was extracted in a phosphate buffer. After centrifugation, by adjusting pH, peptides from the supernatant were isolated. Gel filtration (G-25) and Ion-exchange chromatography on the seed extract gave fairly pure peptide fractions of which inhibitory activities were done by agar well diffusion method against Shigella flexineri, Bacillus cereus, Salmonella typhimurium, Staphylococcus aureus, Enterococcus faecalis, Vibrio cholerae bacterial pathogens. The results indicate that a 30kDa of the peptide was identified and exhibited good antibacterial activity against bacterial pathogens. Among all strains, Shigella flexineri and Staphylococcus aureus exhibit good results with a clear zone of...
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Posted by admin on Apr 30, 2023 in |
The objectives of the present study were to determine the total phenolic content, flavonoid content, and anti-cataleptic activity of chloroform and methanolic extract of the roots of Quisqualis indica. The crude extracts were investigated for total phenolic and flavonoid content using the Folin-Coicalteu method and the Aluminum chloride colorimetric method. It was found that the total phenolic content is greater than the flavonoid content in Q. indica roots. The total phenolic content of a methanolic extract is greater than the chloroform extract as 61.83 μg/ml and 25.40μg/ml, respectively. The flavonoid content in methanolic and chloroform extract was found to be 10.22μg/ml and 2.11μg/ml. The anti-cataleptic activity of the Q. indica roots extracts was evaluated by Wood block test. Catalepsy was induced by clonidine 1 mg/kg, subcutaneously and Chlorpheniramine maleate 10 mg/kg i.p as the standard drug. The vehicle-treated group has shown maximum duration of catalepsy (190.33±3.68) at 180 minute after the administration of clonidine. There was significant inhibition of clonidine induced catalepsy in the animal pre-treated with methanol and...
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Posted by admin on Apr 30, 2023 in |
The current investigation was Developing and validating a novel, simple, responsive, and stable UPLC method for the measurement of active pharmaceutical ingredients of Cytarabine and Daunorubicin. The chromato-graphic strategy utilized a phenyl column of dimensions 100 x 2.1 mm, 1.7 micron, using isocratic elution with a mobile phase of acetonitrile and 0.1 percent formic acid (70:30) with a flow rate of 0.5 ml/min and the run time is 3min and a detector wavelength of 240 nm utilizing the PDA detector was given in the instrumental settings. The Retention time (Rt) of the Cytarabine and Daunorubicin were found to be 0.164min and 1.742 min. The proposed method was validated according to an international conference on harmonization (ICH) guidelines. The accuracy results of Cytarabine and Daunorubicin were found to be 100.4% & 99.8%, and the linearity of Cytarabine and Daunorubicin were 0.992 & 0.990. Precison results of Cytarabine and Daunorubicin were 0.69 and 0.52 and the LOD and LOQ values of Cytarabine 1.65µg/ml, and 5µg/ml and Daunorubicin are 0.726µg/ml and 2.2...
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Posted by admin on Apr 30, 2023 in |
Jaundice is defined as the yellowing of the skin and whites of the eyes which are caused by a buildup of a substance called bilirubin in the blood and tissues in the body. Bilirubin is a yellowish pigment formed by the breakdown of heme, which occurs largely in hemoglobin and red blood cells. The purpose of this study was to analyze and compare the knowledge, attitude, and perception of jaundice among paramedical and non-paramedical students. A Comparative cross-sectional study was conducted in South India for a period of 6 months from May 2021 to October 2021. The Knowledge, Attitude, and perception questionnaire was used to obtain respondents’ information. The data collected were tabulated, analyzed, and interpreted using standard statistical tools. The statistical procedure was undertaken with the help of the statistical package Instat and Prism version 6.0. The comparison was done by the Chi-square test. A total of 818 students were selected and divided into two groups such as 409 paramedical students and 409 non-paramedical students. This study concluded...
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