Posted by admin on Nov 1, 2011 in |
Present study reports CNS depressant and analgesic activities of methanol extract obtained from the leaves of Piper longum L (MEPL). CNS depressant activity was evaluated by using open field and hole cross tests at doses of 250 and 500 mg/kg body weight while peripheral analgesic activity was evaluated by using acetic acid induced writhing method and formalin test respectively in rat model at 100 and 200 mg/kg body weight. The results of the statistical analysis showed that the plant extract had significant (p<0.01) dose dependent CNS depressant and analgesic activities. Locomotor activity and exploratory behavior of rats in hole cross and open field test were decreased in the test group comparing the control group indicating CNS depressant effect of the extract which was comparable with the standard drug diazepam. The extract also showed better analgesic effects at both doses characterized by reduction in the number of writhes in the acetic acid-induced writhing model and reduction of licking time in the formalin test when compared to the control group. The...
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Posted by admin on Nov 1, 2011 in |
Aims The presence of carbonyl groups in proteins has been used as a marker of ROS-mediated protein oxidation. The present work was undertaken to study the importance of endogenous antioxidants like Vitamin C and vitamin E in different types of leprosy patients. Methods: Total 30 leprosy cases were studied. Patients were subjected to various tests like, serum protein carbonyl as an index of protein oxidation, while serum Vitamin C and Vitamin E levels as endogenous antioxidants. Results: An increased level of protein carbonyl indicates oxidative stress .The levels of Vitamins C, E decreased significantly in leprosy. Conclusions: Protein carbonyl can be used as sensitive indicator of oxidative stress in leprosy patients. Vitamin C and vitamin E can be supplemented along with anti-leprosy drugs to control the protein oxidation in leprosy...
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Posted by admin on Nov 1, 2011 in |
In this study, a discriminative dissolution method was developed for Cephalexin OD (orally disintegrating) tablets 750mg. The solubility and stability of the cephalexin API was determined in ten different solutions. In that 0.01N HCl, glycine buffer pH 3-0, acetate buffer pH 4.5 and water gave good stability and the solubility. Dissolution profiling of cephalexin OD tablets 750mg of single batch was done with the selected media containing varying concentration of surfactants (tween 80 and Sodium lauryl sulphate- SLS). The release profile is compared with that of the control media. The media that gave discriminately faster release than that of the control were found to be 0.01N HCl with 0.75% of tween 80, 0.01N HCl with 0.5% of SLS, acetate buffer with 1% of tween 80 and water with 0.5% of SLS. With the selected media, dissolution profile was done on the three different batches of cephalexin OD tablets one with lesser polymer ratio and other with higher polymer ratio that that of the test batch. Only 0.01N HCl with...
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Posted by admin on Nov 1, 2011 in |
The stems of Cissus quadrangularis L. (Vitaceae) are reported to have great medicinal value. The present investigation was therefore undertaken to determine the requisite pharmacognostic standards for evaluating the plant material. The macroscopic features of the Cissus quadrangularis L. stem were observed under magnifying lens. Microscopic characters and powder analysis were determined under microscope. The physiochemical properties such as loss on drying, total ash value, acid insoluble ash value, water soluble ash value, pH, and extractive values of stem were carried out. The microscopic study showed abundant large cells of mucilage, clusters and bundles of acicular crystals of calcium oxalate scattered throughout the section. The powder microscopy showed the presence of annular, reticulated and boarded pitted thickening xylem vessels, cluster, rosette and acicular crystals of calcium oxalate and starch grains. Phytochemical analysis showed the presence of many important classes of phytoconstituents like alkaloids, flavonoids, cardiac glycosides and triterpenes. The determination of these characteristics will aid future investigators in their pharmacological analyses of this...
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Posted by admin on Nov 1, 2011 in |
The primary objective of this study is to evaluate the effect of drug loading and the effect of excipients on the release pattern of Dexamethasone Sodium Phosphate from in situ PLGA implants formed in vitro in gelatin gel. This system is prepared by dissolving a biodegradable polymer (DL-PLGA 70K) in dimethyl sulfoxide (DMSO). Then the drug with excipients or without excipients was added to it. When the drug solution poured into the hallow of gelatin gel, the solvent dissipates into the surrounding gelatin base through diffusion leading phase separation & subsequent coagulation of the polymer and the drug to form a rod like implant in situ. Two types of implants were prepared such as implants containing Dexamethasone Sodium Phosphate and implants containing Dexamethasone Sodium Phosphate with biocompatible excipients such as Tween 20, Tween 60, Span 20, Span 80, Chremophore EL, Chremophore RH40, Stearyl alcohol, Cetyl alcohol, PEG 6000, Stearic acid, GMS, Benzyl Benzoate, Magnesium stearate, Dextrose, Lactose and Arachis oil were used. In vitro dissolution studies were performed in...
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