Posted by admin on Mar 1, 2012 in |
A rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of zoledronic acid in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on an ODS C18column (250 x 4.6 mm x 5 m length),using a mixture of methanol and 0.01M phophate buffer (pH-3) (60:40 v/v) as the mobile phase at a flow rate of 0.8 mL/min and the detection was done at 210 nm. The retention time of the drug was 3.812 min. The method produced linear responses in the concentration range of 0.25 to 60 g/mL of zoledronic acid. The method was found to be reproducible for analysis of the drug in parentral...
Read More
Posted by admin on Mar 1, 2012 in |
The purpose of the study was to find out the cytotoxic activity of the n-hexane, chloroform and carbon tetrachloride fractions of the ethanolic extracts of the leaves and stems of Baccaurea ramiflora (Lour.). Ethanolic extracts of the leaves and stems of Baccaurea ramiflora were subjected to solvent-solvent partitioning using n-hexane, chloroform and carbon tetrachloride to obtain n-hexane leaves fraction, chloroform leaves fraction, n-hexane stems fraction, chloroform stems fraction and carbon tetrachloride stems fraction. Each fraction was assayed for their cytotoxic effect using brine shrimp lethality bioassay. Among the fractions, the n-hexane fractions of leaves and stems showed significant cytotoxic effects having LC50 values of 7.79 µg/ml (95% confidence interval 6.48-9.37) and 5.78 µg/ml (95% confidence interval 4.76-6.99) respectively as compared to vincristine sulfate (LC50= 2.81 µg/ml (95% confidence interval 1.97-4.01) which was used as positive control. The results support the traditional uses of B. ramiflora for various medicinal purposes and thus demand the isolation and identification of active principles and thorough...
Read More
Posted by admin on Mar 1, 2012 in |
Ziziphus mauritiana belongs to family Rhamnaceae and commonly known as Indian jujube or ber. The leaves are alternate and elliptic. Flowers are small and bisexual. The leaves are about 2.5 – 3.2 cm long. Commercially it is cultivated in China & India. Ziziphus mauritiana is small to medium sized spiny tree. The chemical compositions of the leaves are proteins & amino acids, flavonoids, alkaloids, glycosides, terpenoides, saponins, fibers, tannins and phenolic compounds. Leaves are used in the treatment of diarrhoea, gastric disorder, fever, liver damage and pulmonary disorders. The present work deals to summarize the Pharmacognostical studies like ash value, extractive value, moisture content, volatile matters, crude fibers, phytochemical test, fluorescence analysis and micro chemical test. The alcoholic and aqueous extract of Ziziphus mauritiana leave shows hepatoprotective, antioxidant and other important pharmacological activities. The results of above parameters show the clue for more medicinal properties of Ziziphus...
Read More
Posted by admin on Mar 1, 2012 in |
The aim of present study was to investigate the potential roles of ethanolic and hot water extracts of Andrographis paniculata on reducing the blood sugar level (BSL) in alloxan- induced rats. Oral administration of ethanolic (2 g/kg b. w.) and hot water (0.8 g/kg b. w.) extracts of Andrographis paniculata showed the antidiabetic properties and decreased the blood glucose level by 33.71% (p<0.0001) and 39.69% (p<0.0001) respectively in alloxan induced (40mg/kg b.w.) rats. The results were also compared with that of diabetic control...
Read More
Posted by admin on Mar 1, 2012 in |
The present experiment was aimed to examine the effect of furosemide (loop diuretic) on ingestive behavior & some hematological parameters. The adult Wistar rats (n=6/ group) were divided into control group (n =6; Group I) and experimental group (n =6; Group II). The experimental group received furosemide intraperitonially (40mg/kg BW) for a duration of 30 days. The control group of rats was injected with distilled water of same volume intraperitonially. Body weight, food and water intake was measured each day. At the end of the experimental procedure; blood samples were collected to measure the hematological parameters such as total leukocyte count (TLC), blood cell (RBC) count , Packed cell volume (PCV) and hemoglobin (Hb) concentration. The results were analyzed statistically by using student‘t test. P < 0.05 was considered as significant. Our present results showed that, administration of furosemide showed a significant increase (P < 0.001) in the ingestive behavior and body weight. Further, the RBC count, PCV, Hb concentration also showed a significant increase (P < 0.05) whereas...
Read More
