Posted by admin on Jul 1, 2011 in |
A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of atorvastatin calcium and ubidecarenone in tablet formulation. The separation was achieved by 250×4.6 mm, 5µ, L-7 pack (peerless C-8) column and Methanol : Acetonitrile (80:20) as mobile phase, at a flow rate of 1.5 ml/min. The objective of this validation of an analytical procedure is to demonstrate that the drug Atorvastatin calcium and Ubidecarenone is suitable for its intended purpose. Developed method was found to be accurate, precise, selective, economical and rapid for simultaneous estimation of atorvastatin calcium and ubidecarenone in...
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Posted by admin on Jul 1, 2011 in |
The aim of this study was to determine the antimicrobial activity of three selected Lebanese plants (Rheum rhaponticum, Olea europaea, and Viola Odorata) against Extended Spectrum Beta Lactamase (ESBL) – producing Escherichia coli and Klebsiella pneumoniae, and to identify the specific plant fraction responsible for the antimicrobial activity. The plants were extracted with ethanol to yield the crude extract which was further subfractionated by different solvents to obtain the petroleum ether, the dichloromethane, the ethyl acetate and the aqueous fractions. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) were determined using broth microdilution. The MICs ranged between 2.5 and 80 μg/μl. The majority of these microorganisms were inhibited by 80 and 40 μg/μl of the crude extracts. The dichloromethane fraction of Olea europea exerted a significant inhibitory effect on 90% of the tested strains. Ethyl acetate extracts of all selected plants presented antibacterial activity with high potency. Aqueous extracts of Rheum rhaponticum and Olea europaea exerted antimicrobial activity against the majority of the tested strains while Viola...
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Posted by admin on Jul 1, 2011 in |
The study was undertaken to investigate the hypolipidemic and hepatoprotective effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of ethanolic extract of Brassica oleracea. The different fractions of extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced diabetic rats and found to reduce blood lipid level (Total cholesterol and Triglycerides) significantly (p<0.05). The plant fractions also exhibited correction of altered biochemical parameters viz., SGOT and SGPT levels in diabetic rats. The effect of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpenes, alkaloids and flavonoids etc. were present in the plant which has hypolipidemic and hepatoprotective properties. Thus, this study indicates that various fractions (Petroleum ether, ethyl acetate and chloroform) of the ethanolic extract of Brassica oleracea have favorable effect in hypolipidemic and hepatoprotective...
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Posted by admin on Jul 1, 2011 in |
An ethnobotanical survey was carried out in Kovilpatti, Thoothukudi District, Tamil Nadu, India, for the documentation of important medicinal plants used for the treatment of rheumatism. The plants were collected according to their family, local name, vernacular name and useful parts and medicinal uses. There are eight plants are mostly used in this area to treat...
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Posted by admin on Jul 1, 2011 in |
The present study outlines a systematic approach for Formulation and Evaluation of Immediate release Tablets of lamivudine and Zidovudine , Nevirapine- HAART triple therapy. The objective of this regimen is, To delay disease progression, To increase the duration of survival by achieving maximal and prolonged suppression of HIV replication, To restorate and preserve immunological function. Combination therapy is more effective and has less chances of developing resistance than monotherapy. To achieve this goal various prototype formulation trials were taken and evaluated with respect to the various quality control tests such as Thickness, hardness, weight variation, dissolution, disintegration, hardness and assay. The formula was finalized by comparing the Invitro dissolution profile with that of the Marketed Tablets. The in vitro release study was performed in 0.1N HCl upto 60 min. Among all the formulations, formulation F7 release profile was good as compared to the marketed products. Stability studies (40±2ºC/75±5%RH) for 2 months indicated that no characteristics changes in formulation. There was no chemical interaction between drug and...
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