Posted by admin on Oct 1, 2011 in |
Context: The leaves of Nyctanthes arbor-trisitis (Linn.) are used in Traditional System of Medicine for the treatment of stomachic, carminative, intestinal astringent, expectorant, biliousness piles, hair tonic and wound healing. Objective: To evaluate wound healing activity of Nyctanthes arbor-trisitis (Linn.) Material & Methods: Extracts from the dried leaves of Nyctanthes arbor-trisitis (Linn.) were prepared using methanol as solvent in order to investigate the wound healing activity in vivo. Circular excision and linear incision wounds were created on rats. Three groups of rats were prepared viz. control, standard and treated with extract. The methanol extract of Nyctanthes arbor-trisitis (Linn.) was tested for wound healing activity. Results & Discussion: The wounds were monitored and the area of wound was measured on 4, 8, 12, 16 post-wounding days and the mean % wound closure were reported. Epithelization period was calculated as the number of days required for falling of the dead tissue remnants without any residual raw wound. Wound healing rate was measured using formula (Muthusamy et al., 2008). Significant wound healing...
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Posted by admin on Oct 1, 2011 in |
Chonemorpha grandiflora, (Roth) M.R. and S.M. Almeida syn C. fragrans (Moon), Alston (Apocynaceae) has been included in the list of an endangered medicinal plants. It is used in medicinal preparations like kumaryasavam and sudarsanasavum used as tonic3. Entire plant, roots and root bark are used for fever and stomach disorders. The plant is useful in treatment of skin diseases and inflammations. Phytochemical investigations were carried out using in vivo and in vitro plant material of C. grandiflora collected from Dandeli, Uttara Kannada district, Karnataka state. Percentage of total alkaloids was found to be more in stem with bark as compared to the leaves whereas percentage of total alkaloids in callus was found to be more as compared to in vitro plantlets. TLC studies revealed different alkaloid profile in callus as compared to in vitro plantlets. HPLC analysis revealed the presence of camptothecin, an important anticancer compound in the ethanolic extracts of stem with bark of Chonemorpha grandiflora from Thrissur district, Kerala state (Kulkarni et al, 2010). The presence of...
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Posted by admin on Oct 1, 2011 in |
In order to have a good coordination between the quality of raw materials, in process materials and the final products, it has become essential to develop reliable, specific and sensitive quality control methods using a combination of classical and modern instrumental method of analysis. Standardization is an essential measurement for ensuring the quality control of the herbal drugs. “Standardization” expression is used to describe all measures, which are taken during the manufacturing process and quality control leading to a reproducible quality. Kutajaghana vati is official in Ayurvedic formulary of India and is prescribed for the treatment of diarrhea, Irritable bowel syndrome. It is a polyherbal preparation containing two ingredients. In this research paper, an attempt has been made to develop standardization methods of Kutajaghana vati. In-house preparation and the marketed drug have been standardized on the basis of macroscopic, microscopic, physic-chemical parameters and Thin Layer Chromatographic study (TLC). The set parameters were found to be sufficient to evaluate the Vati and can be used as reference standards for the...
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Posted by admin on Oct 1, 2011 in |
Pioglitazone hydrochloride is a novel antidiabetic drug in thiazolidinediones group and it improves insulin sensitivity in insulin resistant patients. One of the major problems with this drug is its low solubility in biological fluids, which results into poor bioavailability after oral administration. The present study is an approach to enhance the dissolution rate of pioglitazone HCl by preparing solid dispersion (solvent evaporation method) using PEG 6000 as carrier in the ratios of 1:1, 1:2, 1:3 and 1:5 respectively. The drug carrier interaction study was carried out by Fourier Transform Infrared Spectroscopy (FTIR). The prepared solid dispersions were characterized for percentage yield, bulk density, tapped density, Carr’s Index, Hausner’s ratio, angle of repose, drug content, in vitro drug dissolution and stability study. The FTIR study suggesting no interaction between physical mixture of drug and carrier. The solvent evaporation was found to be efficient method to obtained good yield solid dispersions with good flow properties. The drug content was found in the ranges of 72.87±0.31 to 85.23±0.22 %. The solid dispersion...
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Posted by admin on Oct 1, 2011 in |
The present study was to evaluate the in vitro cholesterol esterase enzyme inhibitory and in vitro antioxidant activity of the methanol extract of the leaves of Camellia sinensis (L.). Phytochemical screening of the extract shows the presence of flavonoids, phenolics and terpenoids. The extract shows ability to inhibit the enzyme with IC50 (82.46±0.74µg/ml) whereas that of standard, Orlistat (24.15±0.59µg/ml). Antioxidant and free radical scavenging activity were also assessed by using the methods, IC50 values for Nitric oxide radical scavenging activity (396.83±0.83µg/ml), whereas for standard curcumin (260.38±0.66µg/ml), hydroxyl radical scavenging activity (47.04±2.26µg/ml) and for quercetin (70.99±1.31µg/ml). Moreover, the extract was found to scavenge the superoxide with 50% inhibition at 308.17±23.25µg/ml and standard ascorbic acid at 225.08±2.44µg/ml, IC50 for ferrous chelating ability assay (44.12±4.63µg/ml) and of ascorbic acid (47.25±.89µg/ml). Total content of flavonoids present in 1mg of extract was 19.8±0.11 µg quercetin equivalents/mg. Results indicated that the extract shows potential bioactive compounds which might have a beneficial impact on diseases related to cholesterol synthesis and showed potential antioxidant and free radical...
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