Posted by admin on Jul 1, 2011 in |
Antibacterial, and radical scavenging activities of four crude extracts of Phaleria macrocarpa (Scheff) Boerl.) leaves extracts and its active crude cytotoxic properties in HepG2 cells were investigated. The susceptibility bacteria tests were performed by the Mueller Hinton agar well diffusion method. The Radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, while antioxidant potency was tested by DPPH TLC bioautographic assay. The cytotoxic was measured by the MTT assay against human hepatoma cell lines (HepG2). Crude extract MMD showed an equally good activity against Pseudomonas aeroginosa, and strong activity against Bacillus cereus and Streptococcus aureus, with the inhibition zone having diameter between 15-18mm. Crude extract EMD showed has activity against all bacteria compare others, an equally good activity against Escherichia coli, Klebsiella pneumonia and Streptecoccus ubellis, and has strong activity against Pseudomonas aeroginosa, Bacillus cereus and Streptococcus aureus, with the inhibition zone having diameter between 15mm – 27mm. The lowest activity showed for HMD and CMD (diameter<10mm). Crude extract of MMD and EMD exhibited a strong and moderate...
Read More
Posted by admin on Jul 1, 2011 in |
The plant Kalanchoe pinnata is widely used in ayurvedic system of medicine as astringent, analgesic, carminative and also useful in diarrhea and vomiting. Naturalized throughout the hot and moist parts of India. And lots of phytochemical and pharmacological work has done on leaves of plant but the root part is not focused. Hence we have selected roots for phytochemical analysis which will support further evaluation of pharmacological activity. In this first roots are subjected to chloroform for extraction. And preliminary phytochemical test were performed. Chloroform extract was subjected for HPLC and GC-MS analysis which shows various phytoconstituents present in...
Read More
Posted by admin on Jul 1, 2011 in |
Antibacterial, antifungal and phytotoxic activities of the prepared pyrazine derivatives of benzil (A), 4, 4’-dibromobenzil (B) and α-diketones of triterpenoids skeleton e.g. 2, 3-diketolupene (C) and 2, 3-diketomethyl- dihydrobetulonate (D) were studied. The prepared 1, 4-pyrazine derivatives (E, F, G & H respectively) have been characterized by UV, IR, NMR (1H and 13C), optical rotation, mass spectra and by comparison with authentic samples. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Phytotoxicity was determined against seed growth technique. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The derivatives showed significant antimicrobial activities against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa (bacterial specimen), Colletotrichum camelliae, Fusarium equisiti, Alternaria alternata, Curvularia eragrostidis and Colletotrichum gloeosporioides (fungal...
Read More
Posted by admin on Jul 1, 2011 in |
The present work describes a reverse phase high performance liquid chromatographic method without use of buffer in mobile phase for the simultaneous estimation of Lornoxicam and Paracetamol in combined tablet dosage form. Chromatography was performed on Chromasil C18 (250 × 4.6 mm i.d. and particle size 5 μm) column in isocratic mode with mobile phase containing acetonitrile: 0.2% triethylamine aqueous solution (adjusted to pH 6.54 using 1 % ortho phosphoric acid) in the ratio of 62:38 v/v. The flow rate was 0.8 ml/min and effluents were monitored at 310 nm. The selected chromatographic conditions were found to be useful in separating Lornoxicam (run time 4.00 min) and Paracetamol (run time 3.05 min). Linearity for Lornoxicam and Paracetamol was found to be in the range of 2-14 μg/ml and 250-875 μg/ml with correlation coefficient of 0.9995 and 0.9991 respectively. Percent recovery of the drugs was found to lie between 99.06-99.64 % and 99.58-100.65 % for Lornoxicam and Paracetamol respectively. The proposed method was validated by different parameters. It was found...
Read More
Posted by admin on Jul 1, 2011 in |
A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of atorvastatin calcium and ubidecarenone in tablet formulation. The separation was achieved by 250×4.6 mm, 5µ, L-7 pack (peerless C-8) column and Methanol : Acetonitrile (80:20) as mobile phase, at a flow rate of 1.5 ml/min. The objective of this validation of an analytical procedure is to demonstrate that the drug Atorvastatin calcium and Ubidecarenone is suitable for its intended purpose. Developed method was found to be accurate, precise, selective, economical and rapid for simultaneous estimation of atorvastatin calcium and ubidecarenone in...
Read More
