Posted by admin on Jul 1, 2011 in |
Mycobacterium tuberculosis is a deadly infectious disease, there is rising death of humans every year because of this disease, availability of genome sequences of Mycobacterium tuberculosis has provided tremendous amount of information that can be useful in drug target and new vaccine development. Sequence similarity provides accurate annotation for genes in newly sequenced genomes. In this present work about 107 hypothetical proteins of Mycobacetrium tuberculosis were taken and its functions were predicted using bioinformatics tools BLAST, BLOCKS, COGs, InterProScan and PFP. From our analysis of 107 hypothetical proteins only two shows 100% functions these proteins may serve as target for few...
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Posted by admin on Jul 1, 2011 in |
In the present experiment effect of electroplating industrial effluent chromium on the behavioral responses of freshwater air-breathing cat fish Mystus cavasius was studied. The toxicity of chromium to aquatic life is strongly influenced by the form of chromium and quality of water. The two most prevalent chromium forms found in plating industry waste water are hexavalent chromium (Cr+6) and trivalent chromium (Cr+3). The fishes exhibited various behavioral responses – locomotory, physiological, neurological and morbidity responses under influence of chromium industrial effluent. One of the major advantages of using data on behavioral effects is that they are more sensitive indicators of potential for impacts on survival in the filed than are measures of lethality. Study noted the possible utility of the behavior responses as rapid biomonitory assessment technique for qualitative evaluation of various industrial...
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Posted by admin on Jul 1, 2011 in |
Nowadays there has been considerable interest in developing new routes alternative to injection for delivering macromolecules such as proteins and peptides. However, peptides and protein drugs are degraded before they reach the blood stream and can not cross the mucosal barrier. The mucoadhesive polymer coated nanoparticles can solve these problems. They were prepared by emulsion polymerization. Methyl methacrylate polymerized in the presence of polysaccharide such as carbapol leads to formation of mucoadhesive polymer coated nanoparticles. The mucoadhesive polymers could interact with the mucus glycoproteins which allow the mucoadhesive system to remain adhesive for an extended period of time. Coating nanoparticles with them improved their mucoadhesion. These mucoadhesive polymer coated nanoparticles are suitable for carrying hydrophilic drugs. In present study carbapol coated nanoparticles are prepared by emulsion polymerization technique. Particle size was measured by Scanning electron Microscope. The effect of polymer concentration and chemical initiator concentration on the resultant nanoparticles was...
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Posted by admin on Jul 1, 2011 in |
The study is aimed towards studying synergistic antimicrobial effect of cow urine and Azadirachta indica against bacterial, fungal and yeast strains. Mother Nature is the best healer. Last few decades have seen tremendous development in pharma sector. Even though these industries have produced a large number of new drugs and new antibiotics and so does the resistance among microbes is growing. Bacterial strains used were Streptomyces aureofaciens (MTCC 325), E.coli (MTCC 448), Streptococcus mutans (MTCC 497) and Pseudomonas aeruginosa (MTCC 7093). Fungal strains used were Candida albicans (MTCC183) and yeasts like Candida paraopsilosis (MTCC 1965), Candida tropicalis (MTCC 184), and Candida glabrata (MTCC 3019). When equilibrated amount of cow urine is used with Azadirachta indica remarkable synergistic effects were observed in the case of C. tropicalis, C. glabrata, P. aeruginosa, S. aureofaciens....
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Posted by admin on Jul 1, 2011 in |
Orthosiphon stamineus (Family: Lamiaceae) has becoming one of the popular medicinal plants in Malaysia due to its high beneficial effects in treating diabetes mellitus, hypertension and kidney stones. The present study was conducted to evaluate the mechanism of O. stamineus in affecting metabolism of aminopyrine in female rat hepatocytes via second messenger pathway. Liver perfusion was used to isolate hepatocytes from the healthy female Sprague Dawley (SD) rats. Isolated perfused rat hepatocytes were pretreated with several enzyme inhibitors/stimulant targeted on the protein kinases, phosphatase and phosphodiesterase. From the results obtained, only the activity of aminopyrine N-demethylase in female rat hepatocytes pre-treated with KT5720 showed significantly increased in the presence of 1 ng/ml (P<0.05) of O. stamineus extract. For conclusion, 1 ng/ml of O. stamineus extract increased the metabolism of aminopyrine in rat hepatocytes by inhibiting the protein kinase- A...
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