Posted by admin on Aug 1, 2011 in |
A new, simple, precise, rapid and accurate RP-HPLC (Reverse Phase – High Performance Liquid Chromatography) method has been developed for the simultaneous estimation of Atenolol (AT) and Amlodipine Besylate (AB) in bulk and in tablet formulations. The chromatographic separation was achieved on Agilent technologies 1200 series HPLC using Inertsil C18, 5 mm, 250 mm x 4.6 mm column maintained at ambient temperature with mobile phase, Buffer: Acetonitrile: Methanol (4:3.5:2.5 v/v/v), flow rate 1.0 ml/min, load volume 10 μl and a run time of 10 min. The UV detection was performed at 225 nm. Buffer was prepared with Triethylamine and adjusted pH to 3.0 with Ortho-Phosphoric Acid. The retention time and mean recoveries obtained for AT was 2.23 min and 100.1%, for AB was 5.97 min and 100.4% respectively. Linearity response was established over the concentration range of 50-150 μg/ml for AT and 5-15 μg/ml for AB. The correlation coefficient for AT and AB was 0.9992 and 0.9998 respectively. The recovery studies ascertained the accuracy of proposed method and the...
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Posted by admin on Aug 1, 2011 in |
A simple and cost effective spectrophotometric method is described for the determination of flupenthixol dihydrochloride in pure form and in pharmaceutical formulations. The drug was highly soluble in methanol so it was selected as the solvent system for the drug. This ensured adequate drug solubility and maximum assay sensitivity. The linearity range for flupenthixol dihydrochloride at its wavelength of detection of 230 nm was obtained as 1-15 µg/ml. The linear regression equation obtained by least square regression method, were Y = 0.0639X + 0.0013, where Y is the absorbance and X is the concentration (in µg/ml) of pure drug solution. The absorbance was found to increase linearly with increasing concentration of flupenthixol dihydrochloride, which is corroborated by the calculated correlation coefficient value of 0.9997. The limit of detection and limit of quantification was found to be 0.324 µg/ml & 0.982 µg/ml. The validity of the described procedure was assessed. Statistical analysis of the result has been carried out revealing high accuracy and good precision. The proposed method was successfully...
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Posted by admin on Aug 1, 2011 in |
Nitrogen plays a pivotal role in growth and differentiation of cells of Phyllanthus amarus Schum. and Thonn. Both form and amount of nitrogen in nutrient medium have significant effects on the rate of cell growth and differentiation. In this study, effects of different inorganic nutrients (KNO3 and NH4NO3) and organic nutrients (glycine) on regeneration of P. amarus were evaluated. Murashige and Skoog’s medium (MS) was used as basal medium along with plant growth hormones and different concentrations of nitrogen. Among the various growth hormones tried, BAP (0.5 mg/l) gave maximum number of shoots (17.33±0.86) after 3-4 weeks of inoculation. However, various strength of KNO3, NH4NO3 and glycine were optimized in the medium in order to get the maximum number of shoots, nodes per shoot, shoot length as well as the size of leaves respectively. Amongst all modifications, less or more, MS medium with standard amount of nitrogen source was found to be most effective. The shoots regenerated on MS medium along with BAP (0.5 mg/l) were subjected to their elongation on the same medium...
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Posted by admin on Aug 1, 2011 in |
n-octacosanol is a normolipidemic aliphatic primary alcohol obtained from the plant Tinospora cordifolia Miers. In the present study, the plant stems were collected from five accessions namely Anniyalam, Chennai, Hassan, Vishakhapatnam and Bangalore between June and August. Later a simple HPTLC method has been established for determination of n-octacosanol in methanolic extracts of all these geographical sources using Toluene: Ethyl acetate: Formic acid (4.5:4.5:1 v/v) as mobile phase. Detection and quantification were performed by densitometric scanning at λ = 555 nm. The results of HPTLC estimation showed variation in n-octacosanol content in all the accessions. The extract from Anniyalam accession showed significantly higher amount of n-octacosanol (6.54%) and that of Vishakhapatnam showed the least (2.28%). Thus the present work is helpful in selecting Tinospora cordifolia yielding highest percentage of...
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Posted by admin on Aug 1, 2011 in |
Substituted Quinoxaline have received considerable attention during last two decades as they are endowed with variety of biological activities and have wide range of therapeutic properties. A literature survey indicates that quinoxaline derivatives possess different pharmacological and biological activities, of which the most potent is anti-inflammatory activity. In view of above literature survey, we thought to synthesize a novel substituted quinoxaline...
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