Posted by admin on Aug 1, 2011 in |
The present work deals with development and standardization of phytochemical screening for quantification of ethanolic extract of medicinal plant leaves of Pongamia pinnata Linn. The scientific parameter is necessary to identify the exact plant material and to find its quality and purity. The present study deals with various, physical evaluation and preliminary phytochemical screening of various successive extracts such as qualitative chemical analysis and HPTLC of extract shows the twelve different peaks confirming that the twelve compounds present in ethanolic extract of Pongamia pinnata. These studies indicated the possible information for correct identification and standardization of this plant...
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Posted by admin on Aug 1, 2011 in |
A validated simple, economic, selective, and stability indicating spectrofluorimetric method was developed for the determination of some antihistaminic H1 receptor antagonist drugs namely ebastine (EBS), cetirizine dihydrochloride (CTZ), and fexofenadine hydrochloride (FXD). The method is based on the reaction of the cited drugs with 2-cyanoacetamide in alkaline medium to give highly fluorescent derivatives measured at 365 nm after excitation at 312 nm. The method was applied for the determination of the studied drugs in their dosage forms. Furthermore, the method was applied for the determination of the drugs in spiked human plasma, and used to reveal the pharmacokinetic characters in a healthy volunteer treated with oral administration of the different dosages of the drugs. The method was utilized to investigate the kinetics of the alkaline, acidic, oxidative, and ultraviolet degradation of the drugs. The apparent first order rate constants and half life times of the degradation products were...
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Posted by admin on Aug 1, 2011 in |
The methanolic bark extract of Mimusops elengi was investigated for its possible analgesic and neuropharmacological activities on mice at the doses of 100mg/kg, 200mg/kg and 400mg/kg body weight. For the evaluation of analgesic activity, tail immersion and acetic acid induced writhing tests were used. The neuropharmacological activity of the extract was screened using hole cross and open field tests to investigate the central nervous system depressant (CNS) activity. In tail immersion test, the extract produced a significant (p < 0.05-0.001) increase of latent time to flick tail compared to control in a dose dependent manner. In acetic acid-induced writhing test, the extract, at a dose of 400 mg/kg, showed a maximum of 65.48% inhibition (p <0.001) of writhing compared to the control while the reference drug Diclofenac-Na inhibited 76.36% of writhing. In CNS depressant activity tests, the extract significantly (p < 0.05-0.001) decreased motor activity and exploratory behavior of mice in hole cross and open field tests respectively. These results suggest that the extract possesses analgesic and CNS depressant...
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Posted by admin on Aug 1, 2011 in |
Moringa oleifera belongs to an onogeneric family of shrubs and tree, Moringaceae. In the Dravidian language there are many local names for Moringa oleifera but all are derived from the generic root “Morunga”. It has anticancer, anti-inflammatory activity and thyroid status regulator efficacies. An experiment was carried out to study the antibacterial activity of different solvent extracts of Moringa oleifera plant by agar well diffusion method against Bacillus subtilis, Escherichia coli, Klebsiella pnemoniae, Shigella dysentriae and Staphylococcus aureus that frequently cause enteric infections in humans. The benzene, methanol and aqueous extracts of Moringa oleifera leaves, bark, seed and flesh have shown strong antibacterial activity against all the organisms tested such as B. subtilis, E. coli, K. pneumoniae. S. dysentriae and S. aureus. The zone of inhibition ranged from 7-23mm. The plant Moringa oleifera can be used as a source of oral drugs to fight infections caused by susceptible...
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Posted by admin on Aug 1, 2011 in |
Rizatriptan is a selective 5-HT1B/1D agonist which is used in the treatment of migraine headaches. Zero order spectroscopic method has been developed for the determination of rizatriptan. Rizatriptan in its 0.1 N HCl solutions was determined at the wavelength ranges of 220–400 nm by the spectroscopic method. Rizatriptan showed an absorption peak at 226 nm. Linearity ranges were found as 1-5 µg/ml. for the zero order UV spectroscopic method. Limit of quantitation was determined as 0.0944 µg/ml whereas limit of detection was calculated as 0.0311 µg/ml for the same measurements, respectively. No interference was found from tablet excipients at the selected wavelengths and assay conditions. Developed method was found to be validated and showed good precision and reproducibility. Proposed method was successfully applied to the assay of rizatriptan in pure and tablet dosage forms and assessed as to be rapid, sensitive, accurate and relatively...
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