Posted by admin on Jul 1, 2011 in |
Background: Type 2 Diabetes is a metabolic disorder, characterized by chronic hyperglycemia. It results from insulin resistance, a condition in which the body cells fail to use insulin properly. In Ayurveda Sesamum alatum L. (Pedaliaceae) has been used for the treatment of Diabetes. In this study, the antidiabetic activity of the Methanolic leaf extract of S. alatum was investigated, and the renal protective activity of the extract was also evaluated in diabetic rats. Materials & Methods: Antidiabetic activity of the Methanolic Leaf Extract of Sesamum alatum (MESA) was evaluated in the Streptozotocin (65mg/kg) induced Male albino rats. Blood was collected from the Retro orbital plexus of rats’ before and after the administration of MESA. Activity of 300 and 500 mg/kg doses of MESA were evaluated for a period of 30days. Urine samples were collected and analyzed for Albumin, BUN, Creatinine and Lipid parameters. In addition, Kidney MDA levels, Antioxidant enzymes (SOD, CAT) and Non-Enzymatic (GSH) levels were measured. Results: Dose-dependent reduction in blood glucose levels were observed in treated...
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Posted by admin on Jul 1, 2011 in |
A reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of amlodipine and lisinopril in marketed formulation is developed. The determination was carried out on a Phenomenex C18 (250 x 4.6 mm, 5 µm) column using a mobile phase of 0.02 M phosphate buffer solution: methanol (75:25v/v, pH 7.0). The flow rate was 1.0ml/min with detection at 212 nm. The retention time for amlodipine was 4.11 min and for lisiopril 7.29 min. Amlodipine and Lisinopril showed a linear response in the concentration range of 10-110 µg/ml. The correlation co-efficient (‘ r ‘ value) for amlodipine and lisinopril was 0.9991 and 0.9992, respectively. The results of analysis have been validated statistically and by recovery studies. The percentage recoveries obtained for amlodipine and lisinopril ranges from 100.04 to...
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Posted by admin on Jul 1, 2011 in |
Methanol extract of leaves of Spilanthes calva (DC) was examined for their antimicrobial and cytotoxic properties. To determine antibacterial activities, the extract was tested against four gram-positive and six gram-negative bacteria at three concentrations (500, 800, 1000 µg disc-1) through disc diffusion method. The extract showed moderate antibacterial actions and that was increased by increasing the concentration of the sample. The maximum antimicrobial potential was obtained against Staphylococcus aureus and no sensitivity was found for Escherichia coli. Comparatively gram-positive bacteria demonstrated more susceptibility to the extract than gram-negative bacteria. Cytotoxic property of the sample was done using Brine shrimp lethality bioassay where it did not show noticeable toxicity. So, our present study reveals that the leaves extract of S. calva possess considerable antibacterial properties along with lesser amount of...
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Posted by admin on Jul 1, 2011 in |
Antibacterial, and radical scavenging activities of four crude extracts of Phaleria macrocarpa (Scheff) Boerl.) leaves extracts and its active crude cytotoxic properties in HepG2 cells were investigated. The susceptibility bacteria tests were performed by the Mueller Hinton agar well diffusion method. The Radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, while antioxidant potency was tested by DPPH TLC bioautographic assay. The cytotoxic was measured by the MTT assay against human hepatoma cell lines (HepG2). Crude extract MMD showed an equally good activity against Pseudomonas aeroginosa, and strong activity against Bacillus cereus and Streptococcus aureus, with the inhibition zone having diameter between 15-18mm. Crude extract EMD showed has activity against all bacteria compare others, an equally good activity against Escherichia coli, Klebsiella pneumonia and Streptecoccus ubellis, and has strong activity against Pseudomonas aeroginosa, Bacillus cereus and Streptococcus aureus, with the inhibition zone having diameter between 15mm – 27mm. The lowest activity showed for HMD and CMD (diameter<10mm). Crude extract of MMD and EMD exhibited a strong and moderate...
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Posted by admin on Jul 1, 2011 in |
The plant Kalanchoe pinnata is widely used in ayurvedic system of medicine as astringent, analgesic, carminative and also useful in diarrhea and vomiting. Naturalized throughout the hot and moist parts of India. And lots of phytochemical and pharmacological work has done on leaves of plant but the root part is not focused. Hence we have selected roots for phytochemical analysis which will support further evaluation of pharmacological activity. In this first roots are subjected to chloroform for extraction. And preliminary phytochemical test were performed. Chloroform extract was subjected for HPLC and GC-MS analysis which shows various phytoconstituents present in...
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