Posted by admin on Mar 1, 2011 in |
A simple, sensitive, high throughput and specific HPLC method with UV detection was developed and validated to according to the FDA guideline for quantification of gemifloxacin in human plasma. The bioanalytical method consists of liquid-liquid extraction and quantification by UV detection. The analyte was separated isocratically on a Cyano column (250 X 4.6 mm, 5µ particle size) with a mobile phase consist of 1% v/v formic acid, acetonitrile and methanol in the ratio of 75:20:5 (v/v). The response to gemifloxacin was linear over range 0.025 to 2.000 μg/mL. The validation results demonstrate that the method had satisfactory precession and accuracy across the calibration range. Intra-and inter-day precession range from 0.676 to 1.222 and from 0.834 to 1.645% (RSD), respectively. The accuracy determine at three quality control samples was within 94.232 to 101.132%. There was no evidence of instability of the analyte in human plasma following the stability study. The method proved highly reproducible and sensitive and was successfully applied in bioequivalence study after oral dosing of gemifloxacin tablet in...
Read More
Posted by admin on Mar 1, 2011 in |
The objective of this study was to review and explore the top fifteen currently herbal remedies used and the historically used herbal remedies in the treatment of psoriasis. Psoriasis is undoubtedly distressing, affected individuals are typically otherwise healthy and thus well suited to thoughtful outpatient care. Recent advances in our understanding of psoriasis have provided parallel advances in topical treatments. Recent advances in our understanding of psoriasis have provided parallel advances in topical treatments. Specifically herbals, there is limited scientific data regarding their benefits and interactions. About 75% of patients have mild-to-moderate psoriasis, amenable to topical treatment is lifetime controlling herbals remedies like Aloe, Cayenne, Chamomile, Dong Quai, Emu oil, Evening prime rose oil, Fish oil, Tea tree oil, Turmeric, Slippery elm, Wintergreen, Shark cartilage, Milk thistle, Glucosamine, Flexseed oil are needed. Herbal remedies for treatment psoriasis diseases to overcome the adverse effect, antagonistic effect and bioavailability of...
Read More
Posted by admin on Feb 1, 2011 in |
Microencapsulation is a well-known method that is used to modify and retard drug release. In this study, the preparation of Zidovudine loaded microcapsule was accomplished in order to assess the suitability of them for production of oral/vaginal sustained release multiparticulate dosage form of Zidovudine.Microcapsules were prepared using the solvent evaporation method. The effect of formulation variable like polymer: drug ratio was investigated on shape and surface characteristics of microcapsules (by scanning electron microscopy), percentage yield and drug entrapment efficiency, mean particle size and drug release profiles (dissolution test). Microscopic examination of microcapsules revealed that microcapsules were well-shaped and nearly spherical. FTIR studies revealed that no interactions were found between the drug and the polymer. The particle size of all the formulations were found to be satisfactory and within the range of 60.06 to 85.28 μm The percentage yield of all the formulation was found to be satisfactory except MC5. The percentage yield varied from 60.16 to 92.52% suggesting that the processing parameters did not affect the yield from the...
Read More
Posted by admin on Feb 1, 2011 in |
The antibacterial properties of methanol leaf extract of Duranta plumieri wasdeterminedagainst Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Proteus mirabilis and Bacillus subtilis as test organisms using the agar disc diffusion method. The minimum inhibitory concentrations were determined using the microbroth dilution method. The methanol extract was subjected to preliminary phytochemical analysis. Free radical scavenging activity of the methanol extract at different concentrations was determined with 2, 2-diphenyl-1picrylhydrazyl (DPPH). E. coli, Staph. aureus, Kl. pneumoniae, B. subtilis and Pr. mirabilis were susceptible to D. plumieri extract with minimum inhibition concentrations of 48 mg/ml, 192 mg/ml, 192 mg/ml, 12 mg/ml and 96 mg/ml respectively. The extract had no activity against Ps. aeruginosa. Alkaloids, glycosides, saponins, steroids and polyphenols (tannins and flavonoids) were detected as phytoconstituents of the methanol...
Read More
Posted by admin on Feb 1, 2011 in |
Earthworm plays a major role in the proper functioning of the soil ecosystem. It acts as scavenger and helps in recycling of dead and decayed plant material by feeding on them. Earthworm increases the soil fertility and is often referred to as a farmer’s friend. Earthworms have been used in medicine for various remedies. In the present investigation, various solvent extracts of an earthworm, Eudrilus eugeniae were prepared and anti-inflammatory activity of these extracts were determined. The petroleum ether fraction possessed maximum anti-inflammatory activity in carrageenan induced albino rats in comparison to 95% ethanol and 0.2 M phosphate buffer (pH, 7.0) extracts. The paw volume was determined and was compared with that of aspirin, a standard anti-inflammatory drug. The results indicates that petroleum ether fraction of earthworm extract possessed similar anti-inflammatory activity as that of...
Read More
