Posted by admin on Feb 1, 2011 in |
Chlorpyrifos (CPF) an Organophosphate insecticide was evaluated for its toxicity to produce reproductive disorders in rats following oral exposure. In the present study CPF was given to the mice at the dose levels of 10 and 20mg/kg body weight by Gavage method. A histological alteration of ovary and uterus was examined under light microscope. Its oxidative stress levels were studied by noting lipid peroxidation levels and hormonal changes (FSH) were also checked to evaluate the fertility rate in CPF induces toxicity mice. LD50 CPF was established for Swiss albino mice. Bioremediation was done to toxic induced mice using crude and extracted curcumin at 100 and 200 mg/kg body weight respectively. In control group, the germinal epithelium was continuous with prominent and well defined different stages of graffian follicles. On the other hand chlorpyrifos (CPF) treated group showed ruptured germinal epithelium with multiple nuclei and matured graffian follicles with degenerated ovum. Corpus leutium cells were observed to be rudimentary. Unusual number of vacuolated spaces was observed in ovarian cortex. Thus,...
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Posted by admin on Feb 1, 2011 in |
A simple high-performance liquid chromatography RP-HPLC and two reproducible ultraviolet spectrophotometric methods were developed and validated for the estimation of nebivolol HCl and valsartan simultaneously in combined pharmaceutical solid dosage form. The wavelength fixed for spectrophotometric measurement of valsartan at 246.6 nm, nebivolol HCl at 280.2 nm for the simultaneous equation and for the Q-analysis measurement the two wavelengths fixed are 246.6 nm and 275.0 nm. The linearity obtained for nebivolol HCl in the range of 0.5-2.5 µg/ml and 5-25 µg/ml for valsartan by spectrophotometric method. The nebivolol HCl and valsartan were analyzed by HPLC using reverse phase C18 column (25 cm x 4.6 mm i.d., 5 µm particle size), by isocratic mobile phase consisted of 50mM ammonium acetate buffer adjusted to pH 3.5 and acetonitrile (30:70 v/v), at a flow rate of 0.8 ml/min in the detection wavelength of 275 nm. The loratidine was used as an internal standard. The linearity and range of nebivolol HCl are 2-12 µg/ml and 5-30 µg/ml for valsartan by HPLC method. The...
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Posted by admin on Feb 1, 2011 in |
The methanolic extract of Nerium indicum showed the presence of alkaloids, tannins, phenols, and starch in phyto-chemical investigation. Total phenolic content of the drug was estimated by Folin Ciocatteu’s method and was found to be 55.20 µg/10 ml. Analgesic activity was performed using three models they are Writhing, Tail-flick activity and Hot plate models. The methanoilc extract showed 44.51% inhibition in Writhing model, 50.09% for 30 sec’s and 62.45% in 60 sec and hot plate method showed 47.31%. Analgesic activity was carried out and the results were encouraging, which stated that the methanolic extract of Nerium indicum showed significant peripheral analgesic activity. The mechanism of analgesic activity action of Nerium indicum may be due to its inhibitiory effect on the synthesis of prostaglandins and...
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Posted by admin on Feb 1, 2011 in |
In the present study a new series of coumarin derivatives have been synthesized by condensation of ethyl acetoacetate and resorcinol. The chemical structures of the synthesized compounds were confirmed by means of IR, 1H-NMR and Mass spectral analysis. These compounds were screened for their Analgesic and Anti-inflammatory activities. Among the synthesized compounds II (a-1 to...
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Posted by admin on Feb 1, 2011 in |
The study was undertaken to investigate the antihyperglycemic, oral glucose tolerance test (OGTT) and glycogenesis effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of methanolic extract of Momordica charantia. The different fractions of the extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced as well as glucose induced diabetic rats and found to reduce blood glucose level significantly (p<0.05). The different fractions of Momordica charantia to the alloxan-induced diabetic rats resulted in the significant elevation of liver glycogen content which was decreased by 50.60% in diabetic control. The plant fractions also improve the glucose tolerance in the glucose induced rats. The effects of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpines, alkaloids and flavonoids etc. were present in the plant which has antidiabetic and glycogenesis properties. Thus, this investigation paves the way for plant based diabetic treatment and indicates that various fractions (Petroleum ether, ethyl...
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