Posted by admin on Apr 1, 2011 in |
The present study undertaken to predict the three dimensional structure of γ-Hexachlorocyclohexane dehydrochlorinase (linA) in Sphingomonas paucimobilis UT26. γ-Hexachlorocyclohexane dehydrochlorinase (linA) catalyzes the initial steps in the biotransformation of the important insecticide γ-hexachlorocyclohexane (γ-HCH) by the soil bacterium Sphingomonas paucimobilisUT26. Stereo chemical analysis of the reaction products formed during conversion of γ-HCH by linA was identified from structural database using homology modeling or comparative modeling approach. Based on the knowledge of the template, a three-dimensional model was predicted and processed in to energy minimization, Ramachandran plot analysis, quality assessment and finally deposited into Protein Model...
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Posted by admin on Apr 1, 2011 in |
The methanolic extract along with some organic soluble fractions of the bark of Dillenia indica Linn. were tested against four Gram-positive and seven Gram-negative bacteria and against three pathogenic fungi. n-Hexane and dichloromethane fractions showed remarkable activities against all the tested bacteria but n-Hexane fraction showed highest activity against Shigella dysenteriae and its zone of inhibition was 15.51 ± 0.75 mm. Other showed moderate or little activity. Methanol crude extract showed highest activity against fungus Candida albicans with a zone of inhibition 13.13±1.75mm. Lowest minimum inhibitory concentration (MIC) values were observed in n-hexane fraction against Shigella dysenteriae and Staphylococcus aureus and were 0.312 in both cases. Lowest LC50 value 19.02±1.16 of n-hexane fraction indicated the highest toxicity in comparison with the other...
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Posted by admin on Apr 1, 2011 in |
Aim: The aim of this prospective study was to know the variable histopathological changes in the fallopian tube after having ectopic gestation and its correlation with predisposing factors. Materials and Methods: 100 fallopian tubes having ectopic gestation for study group and 25 fallopian tubes received after sterilization operation for control group submitted in department of pathology between Jan. 2007- March 2009 were included. The conventional H&E stained microsections were prepared from specimen for extensive study. The sections were examined histopathologically for various pathological lesions by standard criteria. Results: Acute salpingitis was found in 18% of patients. Chronic salpingitis and follicular salpingitis was seen in 39% of patients, while chronic salpingitis with salpingitis isthmica nodosa was found in only 8% of patients. Vessels were sclerotic in 8% of cases. Calcification was found in 7% cases. No case of tuberculosis and endometriosis was observed in our study group. Conclusion: Pelvic inflammatory disease, Salpingitis isthmica nodosa, Acute and Chronic salpingitis are predisposing factors for ectopic tubal...
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Posted by admin on Mar 1, 2011 in |
Context: Nigella sativa Linn. (Ranunculaceae) is native to southwest Asia. This tree species has been of interest to researchers because it has a long history of folklore usage in various systems of medicines. Objective: Pharmacognostic Standardization, Physico and Phytochemical Evaluation of the seeds of N. sativa were carried out to determine its macro-and microscopical characters and also some of its quantitative standards. Materials and Methods: Microscopical studies were done by using trinocular microscope. Total ash, water-soluble ash and acid-insoluble ash values; Alcohol-and water-soluble extractive values were determined for physico-chemical evaluations. Preliminary phytochemical screening was also done to detect different phytoconstituents. Results and Discussion: Microscopically, seed showed epidermis with parenchymatous cells, papillae and reddish brown pigmented layer of cells; endosperm with starch grains, oil globules and embryo. Total ash was approximately, 32 (thirty two) and 3 (three) times more than acid insoluble and water soluble ash, respectively. Water soluble extractive was approximately 1 times more than Ethanol soluble extractive. T.L.C. of Petroleum-ether extract using Benzene: Ethyl acetate (6:1), showed five spots....
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Posted by admin on Mar 1, 2011 in |
Ougeinia oojeinensis (Roxb) Hochr, an ethanomedicinal plant of Western Ghats seen in deciduous forests. The bark is used to treat various ailments in folk medicine. There are no previous reports on pharmacognostical study on this plant. As a promising ethanomedicinal plant, systematic and detailed pharmacognostical studies were carried out. The present study focused on its macro and micro morphological characters of the bark. An attempt was made on to evaluate the physico-chemical parameters like ash values, extractive values, loss on drying and fluorescence analysis of the bark powder are performed. The chemical composition of the ethanolic extract of Ougeinia oojeinensis was determined by GC-MS analysis. The present investigation revealed the immense value of standardization and botanical identification of the plant material for further investigations and forms an important aspect of drug...
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