Posted by admin on Mar 1, 2011 in |
A simple reverse phase liquid Chromatographic method has been developed and subsequently validated for simultaneous determination of Pioglitazone and Glimepiride in combination. The separation was carried out using a mobile phase of phosphate buffer (pH-4.5): Acetonitrile (45:55) v/v and using methanol as diluent. The column used was Inertsil ODS (250 mm x 4.6 mm i.d., 5μm) with flow rate of 1 ml/min using UV detection at 225 nm. The described method was linear over a concentration range of 5-50µg/ml and 5-25 µg/ml for the assay of Pioglitazone and Glimepiride respectively. The retention times of Pioglitazone and Glimepiride were found to be4.6 and 7.7min respectively. Results of analysis were validated statistically and by recovery studies. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Pioglitazone and Glimepiride bulk drug and in its pharmaceutical dosage form....
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Posted by admin on Mar 1, 2011 in |
To deliver zidovudine (AZT) to asialoglycoprotein receptors, drug-containing galactosylated liposomes were prepared. Simultaneously, miconazole nitrate (MCZ) was entrapped in the outer liposomal bilayer of double liposomes for the treatment of opportunistic fungal infections. Galactosylated liposomes were entrapped in double liposomes. The galactose binding Ricinus communis lectin was used for the determination of in vitro ligand binding capacity. Percent cumulative drug release from double liposomes with inner uncoated liposomes (DLMA) and inner galactosylated liposomes (Gal-DLMA) were compared. Cellular drug uptake studies were performed. In vivo antifungal activity, plasma distribution and hepatic localization study were performed in albino rats. Percent cumulative drug release of AZT from inner liposomes was significantly higher with DLMA as compared to Gal-DLMA. MCZ uptake was 4.7 times greater after encapsulation in liposomes irrespective of inner liposome coating and maximum AZT uptake (7.9 fold enhancement) was observed from double bilayer galactosylated liposomes as compared to free drug. MCZ encapsulated in double liposomes was able to reduce CFU (colony forming units) values to a significant extent in various...
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Posted by admin on Mar 1, 2011 in |
Antibacterial activity of methanolic extract of Terminalia arjuna stem bark samples (Apical bark, middle bark and Mature inner bark) was studied with respect to different pathogenic bacteria species Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoneae, Salmonella typhii, Proteus mirabilis and Micrococcus sp. The methanolic extract of apical bark was effective than the middle bark and mature bark in inhibiting the growth of all bacteria. The bacterium Staphylococcus aureus was most sensitive among all the bacterial species studied. Preliminary phytochemical analysis revealed the presence of alkaloids, glycosides, flavonoids, flavanols, phenols, saponins and terpenoids. The concentrations secondary metabolites was found higher in the apical stem bark than the middle and mature stem bark. The percent extract yield was maximum in apical stem...
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Posted by admin on Mar 1, 2011 in |
The traditional system of the medicine remains the major source of the health care. It is safe alternative, lesser cost and better tolerance and its complications. The major defense against free radical compounds found in medicinal plants is in the form of natural antioxidants. The present investigation is an attempt to assess the bioactivity of daily oral administration of methanolic extract of Tinospora cordifolia stem (T.C.S.) (500mg/kg body weight) for 6weeks in normal and alloxan induced diabetic rats. A significant decrease in blood glucose, glycosylated hemoglobin, cholesterol (p<0.05), and increase in body weight and protein (p<0.01) were observed in diabetic rats on treatment with T.C.S. methanolic extract when compared to normal. The activity of the hepatic enzyme hexokinase was significantly increased where as glucose 6-phosphatase and fructose 1, 6- bisphosphatase were significantly decreased (p<0.01) by the oral administration of T.C.S. methanolic extract in diabetic rats when compared to normal. The six weeks treatment of T.C.S. methanolic extract was proved natural antioxidant present in the plant, because the activity of...
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Posted by admin on Mar 1, 2011 in |
The study was undertaken to investigate the hypolipidemic and hepatoprotective effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of methanolic extract of Momordica charantia. The different fractions of extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced diabetic rats and found to reduce blood lipid level (Total cholesterol and Triglycerides) significantly (p<0.05). The plant fractions also exhibited correction of altered biochemical parameters viz., SGOT and SGPT levels in diabetic rats. The effect of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpenes, alkaloids and flavonoids etc. were present in the plant which has hypolipidemic and hepatoprotective properties. Thus, this study indicates that various fractions (Petroleum ether, ethyl acetate and chloroform) of the methanolic extract of Momordica charantia have favorable effect in hypolipidemic and hepatoprotective activities....
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