Posted by admin on Dec 1, 2010 in |
In the present research investigation we extracted the powdered rhizome of Drynaria quercifolia linn by Soxhletion method using different solvents. Then extracts were subjected to preliminary phyto-chemical investigation. The proximate analysis was carried out for the plant rhizome powder. The total ash value was 9.93%, Acid insoluble ash value was 4.49%, and Water-soluble ash value was 6.96% and extractive values of alcohol and water was found to be 9.87% and 13.94%. The materials were subjected to successive extraction with solvents. The solvents used were petroleum ether, chloroform, methanol and water in the ascending order of polarity. Pet ether extract was Light brown color, highly viscous and sticky and the yield was 3.12%, chloroform extract was brownish black color, viscous and sticky and the yield was 5.72%, methanol extract was brown color and sticky and yield was 19.67%, and the water extract was brown and sticky and yield was 16.33%. All the extract was subjected for qualitative chemical evaluation to detect the phyto-constituents present in them. Pet ether extract revealed...
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Posted by admin on Dec 1, 2010 in |
The aim of present study was to investigate the phytochemical screening and to compare the antimicrobial activity of oils of Clove bud and Cardamom. Clove bud was successively extracted by steam distillation and isolated with Dichloromethane. The phytochemical analysis revealed the presence of alkaloids, glycoside, steroids, carbohydrates, terpenoids, tannins and phenolic compound. The dichloromethane extract was chromatographed over silica Gel (60-120) and eluted with pure toluene, toluene: Dichloromethane (9:1), toluene: Dichloromethane (8:2), toluene: Dichloromethane (7:3), fraction were monitored by T.L.C. similar fractions were combined and concentrated .eleven fractions were obtained and were labelled as f1, f2, f3 to f11. Cardamom fruit was successively extracted with petroleum ether. The phytochemical analysis revealed the presence of alkaloids, glycoside, steroids, protein, carbohydrates, terpenoids, tannins and phenolic compound. The Petroleum ether extract was chromatographed over silica Gel (60-120) and eluted with pure Benzene, Benzene: chloroform (9:1), Benzene: chloroform (8:2), Benzene: chloroform (7:3), Benzene: chloroform (6:4), Benzene: chloroform (5:5), Benzene: chloroform (4:6), and with pure chloroform. Fractions were monitored by T.L.C. similar fractions were...
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Posted by admin on Dec 1, 2010 in |
The simple, accurate and precise absorption ratio method has been developed for the simultaneous estimation of amitriptyline HCl and perphenazine in combined tablet dosage form. The λmax of amitriptyline HCl and perphenazine were found to be 240.0 nm and 258.0 nm respectively. For the estimation by Q-analysis method, the λmax those selected were 240.0 nm and an isoabsorptive point for both the drugs as 253.20 nm. The linearity range lies between 2-12 μg/mL for both amitriptyline HCl and perphenazine at their respective wavelengths. Both the drugs were found in good agreement with the label claimed in the marketed formulation. Amitriptyline HCl and perphenazine in standard mixture were determined as 98.34% and 100.30% respectively. In the tablets both the drugs were estimated as 98.55% and 99.98%. Statistical validation of the data has been carried out and it reveals that the proposed method is sensitive and economical too for the routine analysis of the drugs in combined dosage...
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Posted by admin on Dec 1, 2010 in |
The most important factor in developing pharmaceutical drug substances and drug products today is ensuring that the HPLC analytical test methods that are used to analyze the products generate reliable, reproducible and meaningful results. The US Food and Drug Administration (FDA) and United States Pharmacopeia (USP) have each documented the importance of this to the drug development process and have separately increased validation requirements in recent years. A third source, the International Conference on Harmonization (ICH), has added requirements that, when combined with the previous two sources, have led to three different sets of validation requirements leaving us in ambiguity. This paper gives us a clear understanding over the validation requirements that should be satisfied by FDA, USP and ICH....
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Posted by admin on Dec 1, 2010 in |
Various extracts of Ailanthus excelsa bark evaluated for antifungal at a dose 1 mg/ml, anti-inflammatory and analgesic activity at a dose 200 mg/kg and 50 mg/kg of body weight. The experimental methods were used cup plate method for antifungal and formalin induced rat hind paw oedema measured by plethysmograph (mercury displacement method) for anti-inflammatory and Tail flick method for analgesic activity. Flucanazole (1 mg/ml), diclofenac sodium (200 mg/kg, orally) and Novelgin (50 mg/kg, orally) clinically used drugs were used as standards. The ethyl acetate extract showed good antifungal activity against fungal strains A. terrus, A. niger and A. flavus at 1 mg/ml and the remaining extracts showed moderate activity compared to standard flucanzole. Extracts of Ailanthus excelsa bark showed significant anti-inflammatory and analgesic activity in the above...
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