Posted by admin on Jan 1, 2010 in |
Natural products represent a reservoir of diverse templates and are being tapped to outsource novel anticancer agents. Hemidesmus indicus R.Br.(Fam: Asclepidaceae) has been reported to be useful for the treatment of inflammation, cuts, wounds, burns, snake bite, skin and blood diseases, ulcers, immunological disorders . In the present study, the methanolic extract of the roots of Hemidesmus indicus, was investigated against human colon cancer cell line (HT29) to explore its anticancer potential. The effect of Hemidesmus indicus methanolic extract(HIME)on proliferation of HT29 cancer cell line was determined by microculture tetrazolium assay (MTT). The cells were exposed to different concentrations (100, 50 , 25, 12.5 , 6.25, 3.125 and1.5 μg/ml) of HIME or vehicle for 48 h. Cisplatin (5, 2.5 and 1.25 μg/ml) acted as positive control and vehicle (DMSO) as negative control. Following treatment, the cells were exposed to Tetrazolium dye (5mg/ml) for 4 h. The formation of the purple coloured formazan complex was dissolved by adding DMSO (100 μl) and read at 490 nm using ELISA microtiter plate...
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Posted by admin on Jan 1, 2010 in |
The practice of traditional medicine for the control of fertility in most parts of India is based on the uses of plant medicines for many years. The fact that herbal medicines have been employed for such a long time does not guarantee their efficacy and safety. The aim of the present study was, therefore, to carry out phytochemical screening, efficacy and safety studies on one of the traditionally used antifertility plants: Curcuma aromatica Salisb. The secondary metabolites of the rhizomes of this plant were determined. The ethanolic and aqueous extract of the rhizomes of this plant were investigated for their antifertility activity in female rats The identification of the secondary metabolites showed that the rhizomes of the plant contained phytosterols, alkaloids, carbohydrate and saponins. The ethanolic and aqueous extract of this plant at two different doses of 200mg kg -1 and 400 mg kg -1 b.w. prevented the pregnancy. The aqueous extract were found to possess more significant (p<0.001) antifertility activity compared to alcoholic extract. All these observations suggest...
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Posted by admin on Jan 1, 2010 in |
Various extracts of Tectona grandis Linn. bark were screened for antiasthmatic activity by using different in-vivo animal models like clonidine induced catalepsy, haloperidol induced catalepsy, milk induced leucocytosis and eosinophilia. The observation of this study indicated that the Tectona grandis bark having antihistaminic activity inhibited clonidine-induced catalepsy and not inhibited haloperidol-induced catalepsy. The results of these studies indicated that ethyl acetate extract of Tectona grandis Linn. Bark showed significant (p< 0.001) antiasthmatic activity at the dose of 100 mg/kg. The anti-asthmatic activity of ethyl acetate extract can be attributed to antihistaminic (H1-antagonist), antimuscarinic, antiallergic, anti-inflammatory and adaptogenic activity suggestive of its potential in management of...
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Posted by admin on Jan 1, 2010 in |
A simple, sensitive and rapid high performance liquid chromatography method with UV detection (215nm) was developed and validated for the quantification of Levetiracetam in human plasma. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30 v/v), the analyte and internal standard (Zonisamide) were separated using an isocratic mobile phase, mixture of buffer (5mM Di-Potassium hydrogen phosphate anhydrous, pH7.2): Methanol (85:15 v/v) on reverse phase C-8 Kromasil column. The lower limit of quantization was 1µg/mL, with a relative standard deviation of less than 20%. A linear range of 1µg/mL to 40µg/mL was established. This HPLC method was validated with between and within-batch precision of 5.6–8.9% and 3.9-5.3%, respectively. The between and within batch accuracy was 99.9-106.3% and 96.2-102.0%, respectively. Frequently co-administered drugs did not interfere with the described methodology. Stability of Levetiracetam in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 60 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of <10% and was used in pharmacokinetic...
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Posted by admin on Nov 1, 0203 in |
Lippia nodiflora L. is an important medicinal plant of Verbenaceae family and are used traditionally for several ailments. The present study deals with the detailed physicochemical, fluorescence and phytochemical evaluation of aerial parts of Lippia nodiflora L., in order to explore the authentic plant material suitably for its traditional claims. Thephysicochemical evaluations and fluorescence analysis were determined as per standard protocols. Phytochemical constituents of dried plant material were carried out by both qualitative and quantitative methods. Gas Chromatography–Mass Spectrometry (GC-MS) was used to analyze the phytochemical constituents of the methanol extract. Analysis of physicochemical parameters of the powdered aerial parts showed that total ash was approximately two times more than water insoluble ash and alcohol soluble extractive value was higher than water soluble extractive value.Preliminary phytochemical screening showed the positive result for the presence of flavonoids, steroids, glycosides, alkaloids, terpenoids, tannins and phenolics. Quantitative analysis showed that the methanolic extract consist of high phenolic compounds (98.31 ± 0.004 mg GAE/g) followed by total flavonoids (60.88 ±0.001mg QE/g) and flavonols...
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