Posted by admin on Jun 1, 2010 in |
A simple reverse phase HPLC method was developed for the determination of Esomeprazole magnesium present in bulk and pharmaceutical dosage forms. Efficient chromatographic separation was achieved on Kromasil100-C18 stationary phase (250 X 4.6 mm i.d., 5µ particle size) with simple mobile phase combination of acetonitrile: phosphate buffer 55: 45 (V/V) in an isocratic mode at a flow rate of 1.0 mLmin-1 at 301 nm. The retention time was 4.09 min (±0.5). The proposed method has been applied successfully for the analysis of Esomeprazole magnesium either in bulk or pharmaceutical dosage form with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of Esomeprazole magnesium in pharmaceutical dosage...
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Posted by admin on Jun 1, 2010 in |
The objective of this study was to design and develop o/w microemulsion for transdermal delivery of poorly water soluble acyclovir by aqueous titration method. Oleic acid: castor oil (3:1), tween 80, and ethanol were selected as oily phase, surfactant and cosurfactant respectively. The Pseudoternary phase diagrams were constructed by aqueous titration method. The cosurfactant affect the shape and extant of microemulsion regions. Ethanol (cosurfactant) is expected to disorder the interfacial film gave extended microemulsion zones by destabilizing the liquid crystalline phase. Largest Microemulsion single phase region was found at Smix (2:1) than the system at other Smix. Characterization of microemulsion were done for droplet Shape and size, refractive index, pH, Viscosity, drug loading capacity. The mean droplet size of microemulsion was found below 50 nm. The maximum solubility of ACV in microemulsion system was found to be 47.4 mg/ml. The ex-vivo skin permeation studies were done using skin of Wistar albino rat by Franz diffusion cell, and microemulsion formulation MEC1 exhibited highest flux, was found to be 238.1±4.87 µg/cm2/hr,...
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Posted by admin on Jun 1, 2010 in |
Reaction of 1- (1- benzyl- 1H- benzo [d] imidazol- 2- yl) hydrazine (1) with 3-aryl-1-phenyl-1H-pyrazole-4-carbaldehydes (2a-f) in ethanol yielded the corresponding Schiff bases (3a-f). Further, cyclization of compounds 3a- f with thioglycollic acid in benzene in presence of anhydrous ZnCl2 furnished desired novel compounds 3- (1 – Benzyl – 1h – Benzo [D] Imidazol – 2 – L Amino) – 2 – (3 – Aryl – 1- Phenyl – 1h – Pyrazol – 4 – Yl) Thiazolidin – 4- Ones (4a- f) in 41-65% yield. All the synthesized compounds were screened for antibacterial and antifungal activities. Among the synthesized compounds 3b, 3d, 4a and 4f have shown good activity against bacteria P. aeruginosa, S. aureus and P. vulgaris, where as other compounds have shown moderate to poor activity against all the organisms. The compounds 3a and 3c exhibited good activity against fungal strains A. niger and A. flavus. All remaining synthesized compounds exhibited moderate to poor activity against all the organisms. The structures of synthesized compounds have been established...
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Posted by admin on Jun 1, 2010 in |
Celecoxib, a selective cyclo- oxygenase – 2 inhibitor has been recommended orally for the treatment of arthritis and osteoarthritis. Long term oral administration of celecoxib produces serious gastrointestinal side effects. Therefore the aim of the present investigation was to assess the potential of nanosized emulsion formulations for transdermal delivery of celecoxib (CXB), to reduce the side effects produced by oral administration of the drug, to increase the stability and to produce a novel controlled delivery system with better pharmaceutical and therapeutic properties. Optimized oil in water nanosized emulsion of celecoxib was prepared by ultra sonication method. The prepared nano- sized emulsion was subjected to physical characterization, Drug content analysis and stability tests. Skin permeation mechanism of celecoxib from nanosized emulsion was evaluated by In-vitro skin permeation studies, activation energy measurement and histopathological examination. The anti-inflammatory effects of formulations were compared with marketed formulation by carrageenan-induced paw edema in rats. The nanosized emulsions showed acceptable physical properties and exhibited slow drug release. Photomicrograph of skin sample supports the in-vitro skin...
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Posted by admin on May 1, 2010 in |
A simple, sensitive and selective high performance liquid chromatography (HPLC) method with ultraviolet detection (220 nm) was developed and validated for the quantification of Ibuprofen and in human plasma. Following rapid sample preparation, the analytes and internal standard (Diclofenac sodium) were separated using an isocratic mobile phase on a reverse phase C8 column. The lower limit of quantification was 1µg/mL for Ibuprofen. A linear dynamic range of 1µg/mL to 100µg/mL for Ibuprofen was established. This HPLC method was validated with between and within-batch precision of 1.2- 4.7% and 1.8- 4.8 %, respectively. The between and within batch accuracy was 99.4- 104.2% and 99.7- 104.1%, respectively. Frequently co-administered drugs did not interfere with the described methodology. Stability of Ibuprofen in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 60 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of < 5 % and was used in pharmacokinetic...
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