Posted by admin on Aug 31, 2023 in |
D5 Chooranam is a classical Siddha formulation developed by Central Council for Research in Siddha, prescribed for the management of diabetes mellitus. The present work was carried out to assess phytochemical profile and also to explore the antioxidant potential of Siddha drug D5 Chooranam. The Phytochemical profile and antioxidant activity were evaluated in five different solvent extracts (Ethyl acetate, chloroform, ethanol, petroleum ether, and water) of D5 Chooranam. The phytochemical analysis of D5 Chooranam disclosed the existence of various phytochemicals in D5 Chooranam. The amount of phytochemicals present in D5 Chooranam also correlates with the various pharmacological activities as per its therapeutic indications. The considerable radical scavenging capacity of D5 Chooranam, particularly in its water extract, ethyl acetate extract, and fresh aqueous solution, is well justified by the results of all in-vitro antioxidant studies. These results reveal the beneficial effect of D5 Chooranam against oxidative stress associated disorders like diabetes mellitus and its complications. Hence it can be concluded that it may be due to the presence of vital...
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Posted by admin on Aug 31, 2023 in |
Introduction: Bacterial infections are associated with significant morbidity and mortality globally. This scenario is further complicated by the development of antibiotic resistance, which has an adverse impact on the world economy by increasing the treatment cost and prolonging the hospital stay. It also requires use of reserve antibiotics which have higher toxicity. Hence, new antibacterial drugs are urgently needed to combat multidrug-resistant organisms. The present study was designed to synthesize novel 1-(pyridine-2yl)-1H-pyrazole-5-Carboxamide derivatives and to screen them for antibacterial activity. Methods: A simple, multistep procedure was used to synthesize novel 1-(pyridine-2yl)-1H-pyrazole-5-Carboxamide derivatives. They were characterized by LCMS and NMR. Their antibacterial activity was evaluated against four bacterial strains namely E. coli, Pseudomonas aeruginosa, Bacillus cereus and Proteus mirabilis by agar well diffusion method. Ciprofloxacin was used as the reference standard. Results: Nine novel 1-(pyridine-2yl)-1H-pyrazole-5-Carboxamide derivatives (8a-8i) were synthesized by a cost-effective procedure. The yield of the compounds was good, ranging from 73% to 85%. Compounds 8b, 8h and 8i showed moderate activity against E coli, compound 8g against Bacillus...
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Posted by admin on Aug 31, 2023 in |
Wounds are a severe health problem. The development of pharmaceutical dosage forms to ensure patient comfort and safety and optimize treatment effectiveness is of great interest to the pharmaceutical and biomaterials industries. Tridax procumbens (T. procumbens) leaf is a medicinal herb used for treating wounds since ancient times. In this work, a biomaterial film was developed by using PVA with T. procumbens leaf extract via solvent casting method to produce effective wound healing material. According to the hemolytic investigation, films made from T. procumbens leaf extract films were biocompatible. Using L929 mouse fibroblast cells, the cytotoxicity of films was examined using the MTT assay, and the same cell line was also used for a migration assay using the scratch test. The results of the in-vitro wound healing test showed that films containing 12.5 ml T. procumbens dried leaf extract are efficient in reducing the scratch open area, provoking nearly complete closure of the scratches (94%) within 24 hr without cytotoxicity. The 12.5 ml incorporated film demonstrated better wound healing...
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Posted by admin on Aug 31, 2023 in |
A wound is a breakthrough of skin; it disrupts the normal functioning of skin. So, the wound healing process is important to repair the skin. It restores the normal function and structure of the skin. There are several phases of the wound healing process: inflammation, re-epithelization, vascularization, and granulation tissue formation and maturation. The wound healing process can be studied using in-vitro and in-vivo methods. There are many in-vivo methods to study the wound healing process. In-vivo method uses laboratory animals for the study. Animals used are mice, rats, rabbits, and pigs. Recently, Zebrafish have been used as a model to study the wound healing process. Zebrafish are used because it has similar wound closure properties as mammalian embryos. The wound healing can be studied by amputating the caudal fin of fish and recording the regeneration of the fin on days 5th, 7th and 9th post-amputation. This study compares various wound healing agents with the market product (neomycin cream). The various wound healing agents used in this study are...
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Posted by admin on Jul 31, 2023 in |
The present study aimed to determine secondary metabolites, in-vitro antioxidant, and antiulcer activities using various solvent-based Muehlenbeckia platyclada extracts. Solvents based phylloclade extraction was used to determine various secondary metabolites such as phenols, flavonoids, terpenoids and tannins and to assess in-vitro antioxidant activities such as DPPH, ABTS.+, superoxide, hydrogen peroxide, nitric oxide, FRAP, Phosphomolybdenum, metal chelating and reducing power activities. Further, in-vitro antiulcer activities such as α-chymotrypsin enzyme, urease enzyme inhibitory activity, H+ K+ ATPase activity and acid neutralization capacity (ANC) were performed, and the resultant was analyzed using response surface methodology (RSM) to develop an adequate functional relationship between a response of interest (ME) with independent variables (Time, HCL consumed and NaOH consumed). A correlation coefficient was made between secondary metabolites and in–vitro antiulcer activities. Methanolic extract (ME) showed higher levels of secondary metabolites, in–vitro antioxidant activities and was subsequently selected to evaluate its antiulcer potential. A significant inhibition for alpha chymotrypsin (88.84%), urease (68.52%), and H+K+ ATPase (91.75%) was compared with respective standards. RSM results of ME...
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