Posted by admin on Mar 31, 2023 in |
Itraconazole is a triazole antifungal category drug used for the treatment of local &Systemic fungal infections. Itraconazole is not recommended to neutropenic and other immune-compromised patients who have difficulty swallowing the oral capsule formulation, and it has many side effects. Commercially, Itraconazole topical gel preparation is not available in the market; thus, this formulation is made for better patient compliance, reduces the drug dose, and avoids side effects like liver damage and kidney damage avoiding first-pass metabolism. The gel was formulated by preparing nanocomposite with polymer inclusion complex by microwave-induced diffusion technique. Which ultimately results in enhancing the water solubility of nanocomposites. The resultant polymer-based nanocomposite retains the inherent properties nanoparticles, while providing higher stability, and this nanocomposite is incorporated into the gel base. Prepared nanogel Prepared nanogel is characterized by pH determination, Spreadability, Viscosity measurement, In-vitro diffusion, antifungal activity. Among the formulation, F3 was selected as the best formulation having % CDR of 76.57 % & more Antifungal activity than the marketed formulation. The results indicated that nanocomposite-based...
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Posted by admin on Mar 31, 2023 in |
Objectives: Cyclophosphamide is an alkylating agent known for its reproductive toxicity in males. This study evaluates the protective effects of Withania somnifera extract (WSE) on cyclophosphamide (CP) induced testicular toxicity and DNA damage in male rats. Methods: Adult male rats were randomly divided into four groups Group1-Control, Group 2- Cyclophosphamide, Group 3- Withania somnifera extract, Group4- Cyclophosphamide+ Withania somnifera. After the experimental period of 8 weeks, sperm quality, hormonal analysis, and DNA damage studies were carried out. Histopathological analysis of testes was performed for the assessment of spermatogenic disorders. Results: In CP-treated rats, sperm quality was reduced with decreased testosterone levels with increased DNA damage. Co-administration of WSE with CP improved sperm quality, testosterone levels, spermatogenesis and genotoxicity of cyclophosphamide. The histopathological study also restored most of the degenerative and necrotic changes. Conclusions: This study suggests a prominent role of WS against CP-induced testicular toxicity and DNA...
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Posted by admin on Mar 31, 2023 in |
The present study investigated the effect of intake of aspartame in diabetic rats, a widely used artificial sweetener (aspartame), on antioxidant defence status in the rat brain. Adult male Wistar rats weighed 180 ± 20 gm were randomly divided into 4 groups as follows: The first group was control; the second group was given aspartame (ASP) at a dose of 50 mg/kg body weight; the third group was Diabetes (D) (Streptozotocin (STZ) 45 mg/kg body weight); and fourth group was Diabetes rats (STZ 45 mg/kg body weight) administered with ASP (D+ASP) (50 mg/kg body weight). Induction of Diabetes (STZ) through intraperitoneal injection at a single dose. ASP is administered with gavage for up to 30 days. Superoxide dismutase, catalase and glutathione reductase are significantly reduced. In contrast, in lipid peroxidation, a significant increase was observed in the various brain regions of the experimental group rats compared with the control group rats. The results indicate that consumption of aspartame leads to an imbalance in the normal and diabetic rats’ antioxidant/pro-oxidant...
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Posted by admin on Feb 28, 2023 in |
Glutathione peroxidase gene expression profiling has been determined using budding yeast, S. cerevisiae, as a model organism mainly used for extensively reliable microbial studies of various cellular processes evolved in evolutionarily distant species. Roots of five medicinal plants were used: Withania somnifera, Terminalia arjuna, Ranunculus sceleratus, Bacopa monnieri and Acalypha indica. The maceration method exhibited the extraction process, followed by S. cerevisiae culturing at 600nm. Classic Trizol method for RNA extraction of yeast cells. Further, evaluation of the quantity and quality of the isolated RNA before the CDNA synthesis, respectively. Polymerase Chain Reaction was performed using specific conditions, having 60OC as GPx primers annealing temperature. Real-Time Polymerase Chain Reaction of samples was performed where Beta-actin primers as a housekeeping gene. High purity of RNA samples was yielded to be in the range of 1.8-2.0. The size of cDNA PCR products was found to be 118bp. The RT-qPCR results showed high over-expression, i.e., fold change unit of GPx gene in all sample extracts compared to the control yeast. Ethanol and...
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Posted by admin on Feb 28, 2023 in |
Benzodiazepines are psychoactive drugs used to treat anxiety, epilepsy, convulsions, and psychiatric and sleeping disorders. These medicinal compounds possess considerable importance in forensic toxicology due to their tranquilizing, hypnotic, and anticonvulsant properties. Thus, identifying and separating these compounds found significance in pharmaceuticals. The high-performance liquid chromatography (HPLC) technique is employed to separate benzodiazepines. The present research article aims to provide a more straightforward and efficient method for separating benzodiazepines. The thin layer chromatography (TLC) technique follows to separate benzodiazepines containing silica gel 60 F254 as a stationary phase. The seven different mobile phases for the separation of benzodiazepines are employed. The combination of Acetone: Toluene: Ethanol: Ammonia (45:45:7:3) is the best solvent system for separating benzodiazepines among the seven chosen solvent...
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