Posted by admin on Apr 30, 2023 in |
Drotaverine (DRO) is an anti-spasmodic medication inhibiting phosphodiesterase-4, an enzyme that breaks down cyclic adenosine monophosphate (cAMP). PDE4 inhibition causes an increase in cAMP, which causes smooth muscle relaxation. For DRO, especially the HPTLC technique and stability indicating studies through HPTLC method have not been reported properly in any database. The current research focuses only on a significantly improved HPTLC-based exact, accurate, and specific approach for DRO identification in the presence of degradation constituents employing densitometric detection.DRO was spotted in 6 mm bandwidths with a Camag 100L sample syringe (Hamilton, Switzerland) on a silica gel G60 F254 pre-coated HPTLC aluminium plate with a thickness of 250 µm using a Camag® Linomat-IV applicator. The application rate was 0.4 L/s, with a 6 mm separation between the two bands. Linear ascending development was carried out in a 20 cm × 10 cm twin trough glass chamber, which was then saturated with the mobile phase (Toluene: Methanol; 7:3 v/v).The method was found to be linear over the 200-1200 ng/band range. The stability-indicating...
Read More
Posted by admin on Apr 30, 2023 in |
PCOS (Polycystic ovarian syndrome) is a prevalent female endocrine disorder with global occurrence ranging from 6-26%. It accounts for the majority of anovulatory infertility in women of reproductive age due to enlarged ovaries with small cysts formation, which the National Institute of Health has reported as a cause of 30% of infertility cases in females worldwide. Anovulatory infertility is generally treated with ovulation induction therapy using drugs to promote and facilitate ovulation. However, these medications’ varying success rates and numerous adverse effects compel the search for more promising and safer alternatives. In this research study, 10 ovulation induction agents i.e., Tamoxifen, Metformin, Clomiphene, Pioglitazone, Letrozole, Rosiglitazone, Anastrozole, Prednisone, Dexamethasone, and Spironolactone, were selected to analyze their pharmacokinetics, drug-likeness, bioactivity profile, and toxicity profile through the in-silico approach. The results present information about each drug, including its pharmacological competency and level of toxicity. This study may aid in the direction of future research and the development of more effective ovulation-inducing medications....
Read More
Posted by admin on Apr 30, 2023 in |
Objective: This dissertation work aimed to prepare Levocetirizine HCL (LCH) nasal in-situ gel, a second-generation antihistamine, to improve its retention time, sustain release, enhance the bioavailability, and avoid first-pass metabolism. Process: The in-situ nasal gel was prepared to consume Carbopol as a gelling agent, Hypromellose (HPMC) as a thickening or thickness-imparting agent, and buffering agents to maintain pH. An assessment was conducted to determine the solubility, pH, rheological characteristics, consistency of content, adhesive properties to mucous membranes, gel strength, in-vitro release characteristics, the kinetics of release date, isotonicity, and sterility of the sample. Results: All formulations had a clear appearance with good gelling capacity, and drug content was found to be >87%. The pH values of the formulations were found to be acceptable, ranging from 6.2 ± 0.10 to 6.4 ± 0.44, and no irritation of the mucosal membranes was observed. The shearing character was found between 200-1400 at pH 6.4 and 2000-5500 cps at pH 7.4 when measured at 20 rpm. They also showed adequate gel strength of...
Read More
Posted by admin on Apr 30, 2023 in |
Optimizing the cultural conditions to enhance bioactive metabolite production by Streptomyces rubrus VLK-24 isolated from mangrove sediments as well as characterizing these compounds, has been taken up in this study. The culture broth inoculated in Yeast extract, Malt extract, and Dextrose broth and extracted with ethyl acetate exhibited high antimicrobial activity against test microorganisms, including Gram-negative and Gram-positive bacteria as well as fungi. The crude ethyl acetate extract exhibiting high antimicrobial activity was analyzed by Gas Chromatography-Mass Spectroscopy, which evidenced the presence of 39 compounds according to the available library data, NIST MS Search (ver. 2.0). The results of the study revealed the production of diversified metabolites by the strain; hence it could be a possible source for novel antimicrobial...
Read More
Posted by admin on Apr 30, 2023 in |
For the present study, 10 bacterial strains were isolated from agriculture field soils collected from Guntur, Andhra Pradesh, India, and thereafter screened for amylase activity. Among them, the strain DS5 showed a maximum zone of clearance on the starch hydrolysis test was selected for this study. Identification of bacterial strain DS5 was confirmed by 16S rRNA sequencing analysis. The phylogenetic tree constructed on the basis of 16S rRNA sequences revealed that it clustered with the closest members of Bacillus pumilus DS5 and the sequences were deposited in NCBI Gen bank along with accession number MK430437. Further the effect of incubation period, temperature, pH, carbon and nitrogen sources was optimized. Highest amylase production was found after 48 h of incubation at pH 7.0 and 40 ⁰C temperature. Starch proved to be the best carbon source and peptone as nitrogen source for the highest production of amylase by Bacillus pumilus DS5. Supplementation with different nitrogen and carbon source favoured the increased enzyme yield by submerged...
Read More
