Posted by admin on Jan 31, 2023 in |
Dapagliflozin and Metformin HCl Combination is the novel SGLT2 inhibitor used to control Hyperglycemia in type 2 diabetes mellitus. A literature survey reveals that various Analytical methods have been developed for Dapagliflozin in single and combined with other drugs. No QbD-assisted RP-HPLC method is available to estimate Dapagliflozin and Metformin HCl in combination. So, present work describes the development and validation of QbD driven RP-HPLC method for Dapagliflozin and Metformin HCl. The RP HPLC method has been developed using Agilent 1200 series HPLC with Intersil ODS column (250 x 4,6mm, 5 µm). Based on the RP-HPLC method, development Flow rate and pH were selected as CAA and Resolution, Retention time and Tailing of Drug were monitored using Design Expert@13.00. By applying CCD, 9 trials, 2 factors and 3 responses method had been selected for method development for Dapagliflozin and Metformin HCl. The optimum method development was selected based on the criteria of attaining appropriate Resolution, Tailing, and retention time. The overlay plot of responses generates an optimized area as...
Read More
Posted by admin on Jan 31, 2023 in |
The gut microbiome composition directly correlates with the disease’s development and thus with an individual’s health. According to the published scientific literature, gut microbiota is directly linked with developing diseases such as colorectal cancer. On the other hand, Bacteriophages are the group of viruses that infects the bacterial cells typically and can be utilized to manage the dysbiosis of the gut’s microbial population. Here the major focus of this review is to know the applications and processes of bacteriophages in modulating the gut microbiome. According to studies, the modulation of the gut microbiome using bacteriophages has yielded good results. Although a major issue using this technique to modify the gut microbiome is regarding the survival of the phages when coming in contact with the stomach’s highly acidic environment. Consequently, effective phage encapsulation is required. Thus, in this review, we focus on treating colorectal cancer using bacteriophage-mediated modulation of the gut...
Read More
Posted by admin on Jan 31, 2023 in |
This work aimed to evaluate the diuretic activity of Imperata cylindrica (Poaceae) leaves on rat. The extract was administered orally at doses 100, 200 and 400 mg/kg. The 24h urine volume and its pH were measured, natriuresis and kaliuresis were dosed. The results show that I. cylindrica increases diuresis, natriuresis and kaliuresis, but doesn’t affect urine pH. Diuresis of 24 h urine increases from 3.23 ± 0.32 ml in control group, to 5.33 ± 0.38, 6.83 ± 1.23 and 75 ± 1.32, respectively, in rats treated with the extract (P<0.05). Natriuresis increases from 1.21 ± 0.52 mEq/L to 2.38 ± 0.20, 3.15 ± 0.11 and 5.46 ± 0.72 mEq/L (P<0.05). Kaliuresis increases from 0.51 ± 0.71 mEq/L to 0.73 ± 0.65, 1.21 ± 0.51 and 1.75 ± 0.36 mEq/L (P < 0.05). The pH does not change, it is 8.52 ± 1.59 in control group and 8.51 ± 1.59, 8.47 ± 1.57 and 8.49 ± 1.58, in groups treated with the extract (N.S). These results show that Imperata cylindrica...
Read More
Posted by admin on Jan 31, 2023 in |
The word inflammation comes from the latin word called “inflmmare” which means “To set on fire”. It is a complex biological process that is mediated by several chemicals and biological mediators like pollens, irritants, pathogens and damaged cells. The body’s inflammatory reaction destroys such chemical and biological mediators and gets rid of injured tissues. But in chronic state this inflammation seems to produce symptoms that are quite serious and may be life-threatening. Solanum torvum Sw. is a normal weed found in road side and abandoned field very often in Assam, but it’s berries are used by the Tiwa tribe of Morigaon district, Assam, to treat painful inflammation caused due to trauma or any kind of infection. It is quite common among the tribe to coat the inflamed area with fresh raw berry paste of Solanum torvum Sw. for 2 to 3 days, they take the bitter juice of berries as drink. The author have carried out both in-vitro and in-vivo experiment on the anti inflammatory properties of this berry...
Read More
Posted by admin on Jan 31, 2023 in |
Background and Aim: In-silico approach is commonly the method for predicting and confirming drug design. Hence the present study was undertaken to evaluate in-silico the anti-ulcerative activity of some bioactive compounds from Cassia tora and Butea monospora through a molecular docking approach. Method: Docking is performed using 22 runs of the Lamarckian Genetic Algorithm with the grid box sized x, y, and z coordinates of (20.7075) X (38.7045) X (–33.7057) and spacing of 0.375 Å on Autodock suits software 2. The docking data are then evaluated with the Autodock Tools suite software, and the interaction is displayed with the Biovia Discovery Studio Visualizer. Result: Kaempferol (5280863) and Gallic acid (370) of Cassia tora and Butea monospora showed that the value -8.7 kcal/mol and -8.3 kcal/mol for binding energy, respectively, though binding energy of standard of ligand ranitidine (3001055) is -5.4 kcal/mol. Kaempferol and gallic acid are strongly tied to GLU374 ARG846 ARG775 PHE778 ASP779 LEU843 ARG949 ASP851 and TYR799 CYS813 LEU811, respectively, on the active site of the receptor....
Read More
