Posted by admin on Jan 31, 2023 in |
A rapid, sensitive, selective and robust quantitative densitometric high-performance thin-layer chromatographic method is developed and validated for separation and quantification of flavonoids namely rutin, quercetin, kaempferol and myricetin in herbal formulations. The separation is performed on silica gel 60F254 high-performance thin-layer chromatography plates using phase cyclohexane: ethyl acetate: formic acid 12:9:0.4 (v/v/v) as the mobile phase. A precise and accurate quantification is performed using linear regression analysis by plotting the peak area vs concentration in range of 200–1000 ng/band with regression coefficient, R2 from 0.997 to 0.998 for selected flavonoids. The developed method is validated in terms of accuracy, recovery and inter- and intraday study as per International Conference on Harmonisation (ICH) guidelines. The method is found sensitive, accurate and reproducible. Therefore, it can be recommended for marker-based standardization and quality assurance. The present research work is an attempt for simultaneous quantification of rutin, quercetin, kaempferol and myricetin in a single mobile phase. The validated HPTLC method can be further applied to evaluate the quality of any plant reported...
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Posted by admin on Jan 31, 2023 in |
The cases of skin infections are increasing daily, and the conventional treatment loses its strength in treating those skin infections because of resistance of the microorganisms against the medications, including antibacterial as well as antifungal with the topical and oral administration, or both. The advancement of the treatment of treating skin infections by the combination with the synthetic drug (allopathic medicine) with the polyherbal formulation is known as Allopolyherbal formulation, which gives a wonderful result in the treatment of skin infections due to microorganisms (bacteria & fungus) by lowering the side effects and increasing the pharmacological activity of the synthetic drug (allopathic medicine) with the synergistic therapy in targeting the microbes and also shows the better patient compliance with the reliable therapeutic effects. In this research work, the allopolyherbal formulation contains the polyherbal formulation, which includes A. indica (Neem), O. tenuiflorum (Shyamatulsi), P. emblica (Amla), and A. barbadensis (Aloe) which have the potency enriched with antimicrobial activity and the allopathic drug, the antibacterial (chloramphenicol) and antifungal (terconazole) are used...
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Posted by admin on Jan 31, 2023 in |
Hydrogels are cross-linked polymers with the capacity to bulge in an aqueous medium. Because of their extraordinary properties, these are utilized as brilliant drug delivery vehicles that particularly necessitate focusing on the drug to specific sites. On account of the eminent hydration and biocompatibility properties generally utilized in pharmaceutical field, especially cellulose derivatives. In oral medication, many advanced technologies are introduced. More recently pulsatile drug delivery system is meant for the release of drug followed by a predetermined lag time, which is an emerging approach in chronotherapeutics. In the current research work intended to formulate the pulsicapsules of miglitol by utilizing hydrogel plugs which were prepared by cellulose ethers like ethyl cellulose and sodium carboxymethyl cellulose in ratios of drug: polymer, i.e., 1:2, 1:3. Zero size capsule bodies were treated with formaldehyde to adjust the solubility of capsules and pulsicapsules were prepared by filling the bodies with three dosages of immediate release granules of miglitol which were separated by two hydrogel plugs and closed with an untreated cap. The...
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Posted by admin on Jan 31, 2023 in |
Bioremediation of organic compounds and heavy metals has been recognized to be a successful and effective technique in rhizoremediation of soil toxicity using the beneficial microbes which produce different metabolites and enzymes to degrade the toxic compounds/ pesticides available in the soil. In the present study, actinobacterial isolates were explored and identified for bioremediation of soil toxicity available in the soil. The actinobacterial isolates were utilized for the promising PGPR traits and ability to degrade the monocrotophos pesticide residues available in the soil. The “opd” gene of interest responsible for monocrotophos pesticide degradation trait was isolated and cloned in vector DNA to produce recombinant DNA. The recombinant DNA was transformed in E. coli cells to produce multiple copies in E. coli cells resulting in recombinant (transformed) and non-recombinant (non-transformed) colonies. The transformed E. coli cells were inoculated in nutrient broth having pesticide concentration. The transformed cells degraded the pesticide, and the HPLC method determined the derivatives produced. The formulations based on actinobacterial isolates were tested, and field applications were...
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Posted by admin on Jan 31, 2023 in |
Background: Solubility is an important boundary to obtaining the needed drug concentrations in systemic circulation for pharmacological response to be shown. In today’s time, poor water solubility is the crucial issue faced with developing new chemical entities. Objective: Cefixime trihydrate (CT) is an oral third-generation cephalosporin antibiotic used to treat bacterial diseases. It has an oral bioavailability of 40-50% and belongs to the BCS class-IV. This study aims to boost the solubility of poorly aqueous soluble anti-bacterial drug cefixime trihydrate (CT) by the hydrotropic solubilization technique. Methods: Hydrotropy is one of the solubility improvement techniques that enhance the solubility to many folds with hydrotropes. Sodium benzoate (SB) and piperazine (PP) are the hydrotropes that were selected for the preparation of solid dispersion to improve the solubility of cefixime trihydrate. Thus, prepared solid dispersions were evaluated for percentage yield, drug content, saturation solubility and in-vitro dissolution studies. Results: The result showed increased solubility and in-vitro drug release of CT compared to the pure drug. The solubility of cefixime trihydrate was...
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