Posted by admin on Oct 31, 2022 in |
The novel Corona Virus Disease – 19 (COVID-19) is a Contagious disease spreading among Animals and Humans, a Severe Acute Respiratory Syndrome CoronaVirus-2 (SARS CoV2). The First case was identified in Wuhan, China as the disease has unfolded worldwide to the continuing pandemic. It created more infected cases and deaths worldwide, creating an urge for newer drugs of manipulate the Ongoing Pandemic and address the contemporary state of affairs. Remdesivir (GS-5734) has been recognized because the first authorized remedy for intense COVID-19 and has also been recognized to be a novel nucleoside an a log with a large antiviral activity spectrum among RNA viruses which include ebolavirus (EBOV) and the respiratory pathogens Middle East breathing syndrome coronavirus (MERS-CoV), SARS-CoV and SARS-CoV-2. Being first defined in 2016, the drug was derived from an Antiviral library of small molecules intended to target emerging pathogenic RNA viruses. Remdesivir has been discovered to lessen the time to restoration of hospitalized patients who require supplemental oxygen and has a high-quality effect on mortality results...
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Posted by admin on Oct 31, 2022 in |
The pandemic COVID-19 caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has killed millions of human beings throughout the world and attaining natural immunity against this virus is the best remedy. Consumption of nutraceuticals having antiviral and immune-boosting ingredients is the natural way to fight against it. The main objective of the present study was to evaluate the anti-SARS-CoV-2 activity of phytochemicals from Syzygium aromaticum and identify lead molecules through an in-silico approach. To determine anti-SARS-CoV-2 activity, 249 phytochemicals from Syzygium aromaticum (Clove) were docked with each of the four therapeutic targets namely Spike protein (SP), Angiotensin-Converting Enzyme-2(ACE2), Main protease (Mpro) and RNA-dependent RNA polymerase (RdRp). Spike protein and ACE2 have a key role in viral entry into the human host and Mpro and RdRp have a key role in viral multiplication. The docked molecules with minimum binding energies ≤ -6 kcal/mol were considered active/hit molecules. The total number of hits obtained in the order of merit was 46, 51, 60, and 150 against RdRp, spike protein, Mpro, and...
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Posted by admin on Oct 31, 2022 in |
Acyclovir has a ratified antiretroviral effect for Herpes simplex virus type 1 (HSV-1), HSV-2, Varicella zoster virus (VZV), Herpesvirus simiae and to a minor extent, Epstein-Barr virus (EBV). The oral bioavailability of acyclovir is indigent, with only 15%–30% of the oral formulations’ vitality absorbed. To baffle the issue of insignificant bioavailability, acyclovir was formulated into biodegradable gelatin nanoparticles through a double desolvation method adopting gelatin, acetone as desolvating agent, and glutaraldehyde as a cross-linking agent. Optimization was imposed through design expert software whereby the aftermath of gelatin polymer concentration (X1) and glutaraldehyde-crosslinking agent (X2) was studied on particle size (Y1), zeta potential (Y2) and entrapment efficiency (Y3). The optimized formulation (F9) demonstrated a particle size, zeta-potential and maximum entrapment efficiency of 139.87 nm -32.67mv and 91.23%, respectively. The rate of drug release from acyclovir-loaded gelatin nanoparticles ensued from first-order kinetics, and Korsmeyer-peppas plots established the mechanism of drug release from nanoparticles. The release exponent (n) value indicating that drug release embellished by quasi-fickian diffusion transport. The in-vivo pharmacokinetic parameters such as...
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Posted by admin on Oct 31, 2022 in |
Spinacia oleracea L. (child spinach) is a generally new leaf vegetable in the Amaranthaceae family. The crop is high in human supplements and generally has significant degrees of bioactive mixtures like nutrients A also C and minerals. In the present study, the fungi and bacteria from the infected leaves of Spinacia oleracea are isolated and morphological identified. DNA isolation of the cultured fungi and bacteria was done. The isolated genomic DNA was amplified using the internal transcribed spacer region and 16s rRNA region. The new DNA sequences were identified at the genus and species level using the BLAST software tool (NCBI, USA), deposited in (The National Center for Biotechnology Information) NCBI, and got Genbank accession numbers. The DNA sequences that showed maximum similarity to query sequences were selected and analyzed using MEGA 11.0 (Molecular Evolutionary Genetics Analysis) for phylogenetic analysis. The phylogenetic tree was generated based on the Neighbour-joining...
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Posted by admin on Oct 31, 2022 in |
Microspheres drug delivery system have improved patient compliance, decreased toxicity, and increased efficacy. This work aimed to design a controlled-release drug-delivery system for lipid microspheres of Verapamil hydrochloride that would retain the drug in the stomach and continuously release the drug in a controlled manner up to a predetermined time. Lipid microspheres were prepared by using a melt solidification method. Microspheres with spherical shapes and narrow size distribution were formed. In the present investigation, two lipids were used in various concentrations; Ceresine wax & Bees Wax. In-vitro performance was evaluated, such as particle size analysis, drug entrapment efficiency, compatibility study, Preformulation study, flow properties, in-vitro drug release studies, stability studies, etc. The drug in microspheres was found to be stable and compatible with waxes, as confirmed by DSC and FTIR methods. The mixing ratio of lipids with drug affected the size, size distribution, Yield, drug content, and drug release of microspheres. Results showed that the solid, discrete, reproducible free-flowing microspheres were obtained. In most cases, good in-vitro microsphere behavior...
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