Posted by admin on Sep 30, 2022 in |
Aim: The emergence of drug-resistant pathogens enforces the isolation of potent and novel antibiotic-producing Streptomyces sp., from the undisclosed ecological niches. Methodology: Unique Streptomyces violascens strain VS isolated by serial dilution agar plate method and strain confirmed by 16S rDNA sequencing. Antibacterial assay against multidrug resistant Pseudomonas aeruginosa isolated from bronchoalveolar lavage fluid was documented by primary (cross streak method) and secondary screening (agar well diffusion assay). Antibiotics are purified by organic solvent extraction, column chromatography, and thin-layer chromatography. The MIC and MBC value of antibiotics is determined against the pathogen by tube dilution method. Results: Fourteen Streptomyces species were isolated from Erode district, Tamil Nadu, with six different soil samples. Among the isolated Streptomyces sp., AC01 exhibited stable antibacterial activity against the MDR strain both in primary and secondary screening. It produced an opulent growth of powdery white colonies with yellow colour spores arranged in chains. Based on 16S rDNA sequencing, the isolated identified as Streptomyces violascens strain VS. Crude antibiotic was extracted from ISP-2 broth by ethyl...
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Posted by admin on Sep 30, 2022 in |
In the present research work, measurement of viscometric parameters – Density, Molar Volume, Apparent Molar Volume, Viscosity, Specific Viscosity, and Fluidity of synthesized Vanadium (V) complex of commercial antihypertensive drug, Diltiazem has been reported in non-aqueous solvents of varying compositions. Composition of non-aqueous solvents selected for study is 20% and 40% methanol-water mixture. Experimental results showed the effect of Concentration of solute (Diltiazem drug) on density and viscosity in a methanol-water mixture. The density of Diltiazem-V (V) complex showed an irregular pattern in 20% methanol-water mixture, whereas an increase was observed in 40% methanol-water mixture. The molar volume value for the complex solution in both 20% & 40% methanol-water mixture showed rapid increase with the increase in complex Concentration. The apparent molar volumes (φv) decreased with an increase in Concentration of complex and same trend was followed in both 20% and 40 % methanol-water solvent system. Study of the viscous behavior of complex molecules in solution gives an understanding of the transport process mechanism and information about the shape...
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Posted by admin on Sep 30, 2022 in |
Snakebite is an international health hazard that needs attention in terms of effective management and better treatment to recover the health of the affected individuals and society. Tribal populations and folk therapists across the world have been using innumerable plants to treat snakebite difficulties. In the present study, the efficacy of aqueous extracts of Rauvolfia serpentina root for neutralizing Bungarus caeruleus venom by in-vitro and in-vivo methods was carried out. Various in-vitro neutralization tests like Direct and Indirect haemolytic activities, Acetylcholinesterase activity, Proteolytic activity, ATPase activity, and in-vivo assessment LD50 were carried out. Rauvolfia serpentina root extract effectively neutralizes all the venom’s toxic effects. The in-vivo assessment of venom lethality (LD50) of Bungarus caeruleus venom was 0.537 µg/g. Rauvolfia serpentina root extract effectively neutralized the venom lethality, and the effective dose (ED50) was found to be10.96 mg/3LD50 of Bungarus caeruleus. All animals survived and appeared active and healthy throughout the study. The LD50 of Rauvolfia serpentina root extract was >2000mg/kg. These findings confirmed that Rauvolfia serpentina root extract possesses...
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Posted by admin on Sep 30, 2022 in |
Background: The present study was carried to evaluate the phytochemical, antioxidant, and anti-diabetic potential of Abies pindrow leaf extracts. Methods: The extracted leaf samples of Abies pindrow in methanolic and hydroalcoholic solutions were evaluated for phytochemical and antioxidant activities. In-vitro antioxidant activity of both test samples was calculated by DPPH radical scavenging capacity, reducing power activity, total phenolic and flavonoid contents. Further the study was carried to evaluate the anti-diabetic potential of hydroalcoholic extract of Abies pindrow leaves in Alloxan-induced diabetic rats for 21 days. Various biochemical parameters and histopathological studies were also carried out. Results: The hydroalcoholic extract of Abies pindrow was found to have more antioxidant potential than the methanolic extract. Also the same extract at high dose (400mg/kg) exhibited significant anti-diabetic activity than the low dose (200mg/kg) in alloxan-induced diabetic rats. The hydroalcoholic extract also showed improvement in parameters like body weight and lipid profile as well as regeneration of β-cells of the pancreas in alloxan-induced diabetic rats. Histopathological studies show reinforcement of the healing of...
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Posted by admin on Sep 30, 2022 in |
Objectives: Gymnosporia montana (family Celeastraceae), important shrub which is widely reported in Gujrat, India. In this study, our major objective is to screen secondary metabolites of methanolic leaves and fruit extract and determining its antioxidant and anti-inflammatory potential of this shrub. Materials and Methods: Leaves and fruit extract were subjected to methanol followed by phytochemical investigations and estimating the presence of secondary metabolites and also analysing its activity for antioxidant (DPPH and hydrogen peroxide scavenging methods) and anti-inflammatory potentiality were carried out. Results: Preliminary studies related to its phytochemical screening of leaves and fruit extract of Gymnosporia montana in the form of methanol extract which revealed its existence of flavonoids, alkaloids and terpenoids. In comparison with alkaloid and terpenoid extract, there is significant enhancement in antioxidant activity of flavonoid content in case of methanolic extract in a dose-dependent manner. Thereafter, itsanti-inflammatory activity test was carried out against Typhoid vaccine (25 µg/ml, 10 µl) with these extracts (flavonoids, terpenoids and alkaloids of methanol) using variable concentration and compared with Typhoid...
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