Posted by admin on Dec 31, 2022 in |
Current work makes known that Liquid Chromatography-Mass Spectrometry (LC-MS) method development and validation for the impurity G (N, N -dimethylpyridin-4-amine) and impurity S (2-[(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl) methoxy] ethyl N-[1,1-dimetylethoxy) carbonyl]-L-valinate) in Valaciclovir Hydrochloride Hydrate active pharmaceutical ingredient (API). The developed LC-MS analytical method is complimentary to the thin layer chromatography (TLC) method available for impurity G and impurity S quantitation in the Valaciclovir Hydrochloride Hydrate API monograph. Impurity G and Impurity S were determined by the LC-MS method in Q1 Multiple ion mode using Ascentis Express C18 (15cm X 4.6 mm) 2.7µm analytical HPLC column. A gradient system was employed for the elution of analytes using acetonitrile (Eluent B) and 0.01M Ammonium Formate, LC-MS compatible volatile buffer, pH 3.0 (Eluent A) in distinct compositions. The gradient system (T/%B) was applied as 0.01/5, 4.00/5, 7.50/80, 10.00/80, 12.50/5, and 20.00/5. A method developed was validated considering the International Conference on Harmonization pharmaceutical guidelines. The quantitation limit found for Impurity G and Impurity S were 207.20ppm and 216.00...
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Posted by admin on Dec 31, 2022 in |
Glibenclamide belongs to a group of medicines called sulfonylureas. Glibenclamide is used to manage diabetes. Glibenclamide, also known as glyburide, is an antidiabetic medication used to treat type 2 diabetes. The purpose of the present study was to formulate an Oral dispersible tablet of Glibenclamide using the Solid dispersion technique through using polyethylene glycol ratio as a water-soluble carrier. The solid dispersion of Glibenclamide was prepared by using fusion methods. The characterization of Glibeclamide solid dispersion powder was done by using several parameters like bulk density, tapped density, Carr’s Index, Hausners’s ratio, drug content, solubility and in-vitro dissolution study. While the oral dispersible tablet was prepared by using direct compression method. The prepared tablets were evaluated for disintegration time, moisture content, friability, and wetting time. From this study, it can be concluded that fast disintegrating tablets could be a potential drug delivery technology for managing diabetic...
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Posted by admin on Dec 31, 2022 in |
Purpose: The present study has been designed to investigate the protective effect of methanolic extract of Albizia odoratissima (AO) bark in prevention of streptozotocin (STZ) induced-diabetic nephropathy (DN) in Wistar rats. Methods: Healthy Wistar rats of either sex weighing 180-200g were employed in the present study. Experimental diabetes was induced in rats by injecting STZ at a dose of 45 mg/kg (i.p.). Assessment of DN was done by estimating glucose level in blood and urine samples. Moreover, different antioxidant parameters like catalase, superoxide dismutase (SOD) and thiobarbituric acid reactive substances (TBARS) in kidney tissue samples were assessed. Additionally, inflammatory cytokines like interleukin-1 (IL-1), transforming growth factor beta (TGF-β), and tumour necrosis factor-alpha (TNF-α) were assessed in renal tissue. Results: Methanolic extract of AO bark (AOB) has shown significant prevention against diabetes-associated nephropathy. The bark extract decreased glucose levels both in urine and blood sample. The AO extract, either alone or in combination with a standard drug (glibenclamide) showed a significant reduction in oxidative stress in renal tissue, as demonstrated...
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Posted by admin on Dec 31, 2022 in |
Background Diclofenac Potassium is the potassium salt form of diclofenac, a benzene acetic acid derivate, and nonsteroidal anti-inflammatory drug (NSAID) with analgesic, antipyretic and anti-inflammatory activity. Aim: Therefore, the purpose of the present study was to formulate and evaluate liquid-filled hard gelatin capsules of diclofenac potassium. Methods: The liquid-filled soft gelatin capsules were prepared by using PEG 400, propylene glycol and water mixture under temp of 900 C followed by adding PVA, Cremophore EL, Labrasol and in last diclofenac potassium was finally added to the mixture under stirring. The final formulation was filled in a hard gelatin capsule, and the hand band seal was performed. Physico-Chemical Evaluation: A total fifteen liquid-filled hard gelatin capsule formulations were prepared (F-1 to F-15) and evaluated for weight variation test, lock length, drug content, nephelometric turbidity units (NTU) and in-vitro release study. Results: The drug content ranged from 92.76±0.12 to 99.25 ±0.19. The results indicated that the drug content was uniform in all the formulations. The disintegration time ranges from 130 sec to 232 sec. In-vitro dissolution study...
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Posted by admin on Dec 31, 2022 in |
To investigate the hepatoprotective activity of Coccinia grandis fruit extract against anti-tubercular drugs induced hepatotoxicity in rats. 50% ethanolic extract of Coccinia grandis (CGE 200 and 400 mg/kg body weight) was administered daily for 9 days in experimental animals. To develop hepatotoxicity, animals were administered orally with Isoniazid (50 mg/kg, p.o.) and Rifampicin (100 mg/kg, p.o.) at 2 h after the doses of CGE every day for nine consecutive days except for rats in the normal group. The hepatoprotective activity was determined using different biochemical parameters such asalanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin (ALB), Bilirubin (BIL), Cholesterol (CHL) and lactate dehydrogenase (LDH). Histopathological examinations further accomplished the hepatoprotective effect of the CDE. The results obtained demonstrated that the treatment with Coccinia grandis significantly (P<0.05-P<0.001) and dose-dependently prevented the drug-induced increase in serum levels of hepatic enzymes. Histopathology of liver tissue showed that Coccinia grandis attenuated the hepatocellular necrosis, regeneration, and repair of cells toward normal. The results of this study strongly indicate the protective effect of...
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