Posted by admin on May 31, 2022 in |
A novel series 3(a–d) 1-(4-benzhydryl piperazin-1-yl)-3-(di substituted) propan-1-one &1-(4-benzhydrylpiperazin-1-yl)-3-(piperidin-1-yl) propan-1-one (3e) & 1-(4-benzhydrylpiperazin-1-yl)-3-morpholinopropan-1-one (3f) compounds have been synthesized and screened for its anticonvulsant and neurotoxic activity, After i.p. injection to rat at doses of 30, 100, and 300 mg/kg body weight. Synthesized compounds were examined in the maximal electroshock-induced seizures (MES). Spectroscopic data were consistent with the structure of newly synthesized compounds. The neurotoxicity was assessed using the rotarod method. In the prepared series, 3f was found to be active in the MES screen at 0.5 h, and 4...
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Posted by admin on May 31, 2022 in |
Leucas aspera species, auspicious in many cultures worldwide, are sources of novel bioactive secondary metabolites. The mechanism by which they exhibit therapeutic potential via antioxidant, antimicrobial and apoptotic properties is scant. The main objective of the current study was to evaluate the properties of activity-guided fractionated root methanol extract. As the methanol extract exhibited the highest antioxidant activity, it was evaluated for secondary metabolites and therapeutic properties UPLC-MS analysis of the extract identified the 15 and five secondary metabolites including oleanolic acid, 6-C-glucosyl- 8- C- arabinosylapigenin, 6- C-arabinosyl 8- C-glycosylapigenin, neohesperidin and beta-sitosterol were reported. Anti-bacterial activity by disc diffusion inhibited human pathogenic bacteria. Bacterial bio-film inhibition capacity of extract (750 µg) imaged by Confocal laser scanning microscopy revealed loss of microcolonies. Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay, indicating its strong antioxidant property. The MTT assay results identified maximum cell death for human breast cancer cells, MCF-7 (EC50, 60.31±2.71 mg/mL-1). CLSM visualized a clear indication of apoptosis via chromatin condensation. The presence...
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Posted by admin on May 31, 2022 in |
Glinus oppositifolius (Linn) is a branched herb containing linear to obovate, opposite leaves with greenish flowers, and it belongs to the molluginaceae family. This bitter leafy vegetable is known as gima shak in West Bengal, Assam & Bangladesh. This leaf contains various bioactive components which provide protection against diabetes, hyperlipidaemia, joint pain, inflammation, diarrhea, skin disorder, etc. Previous nutritional analysis in our laboratory found that the iron content of these gima leaves is high. A randomized study was conducted among poor adult subjects who are socio-economically backward and have no chronic diseases. 18-65yrs aged people were randomly selected from slum areas of Kolkata. Among them, Fifty-Five samples consumed 50 gm boiled gima paste as supplementation for 45 days. Data on their dietary habits, anthropometry, medication habits, and haematological parameters were analyzed. Supplementation of gima revealed that there is70%&64% increase in haemoglobin among men and women respectively, whereas females of 40-60 years showed more positive changes than men. After supplementation of gima, the iron storage i.e. ferritin level, showed a 76% and 66.6% increase in...
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Posted by admin on May 31, 2022 in |
Background: Covid-19 has become a global threat. All most all the countries are facing this pandemic. This pandemic has greatly affected the medical education and medical students who are tomorrow’s doctors. So, they need to have a wide awareness regarding the knowledge, attitude, and practice towards this Covid-19 infection. Objective: To study the knowledge, attitude, and practice of medical students regarding the Covid–19 pandemic. Material and Method: This descriptive and cross-sectional study was conducted on 260 medical students of J.L.N. Medical College, Ajmer (Rajasthan). A total of 20 questionnaires in which, 10 in the knowledge section, 5 each in the attitude and practice section, were provided in July – August, 2021. Results: A total of 260 responses were collected and studied. The male to female ratio in the study population was 0.91. A total of 54.46% of students gave correct responses regarding the knowledge section. 87.31% of students show a positive attitude towards vaccination. Overall positivity regarding attitude was 76.61%. 95 % of Students were proactive regarding washing their...
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Posted by admin on May 31, 2022 in |
Objective: Active chemical constituent in plants exhibits pharmacological action. Hence this research work aimed to identify and quantitate oleanolic acid. Methods: A simple, high-performance thin-layer chromatography (HPTLC) is developed and validated to identify and quantify oleanolic acid. The extracts were spotted as bands on aluminum TLC plates which were pre-coated with silica gel. Different compositions of mobile phase tried for high resolution and reproducible results. Mobile phase composition toluene: methanol: ethyl acetate (8.5:1:0.5 V/V) utilized. Plates were derivatized with 10% sulphuric acid and scanned. Oleanolic acid was detected quantitatively at 529nm. Result: In the selected mobile phase, Rf value for oleanolic acid was found to be 0.35, which indicated a good separation. The Chromatography method was validated with respect to Linearity range (ng), Intraday and Interday precision, Instrumental Precision, Quantitation Limit i.e., LOQ (ng), Detection Limit, i.e., LOD (ng), Repeatability for oleanolic acid as per ICH guidelines. Oleanolic acid calibration graph in 200-1200 ng range per band was found linear with correlation coefficient value 0.998. All the parameters obeyed...
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