Posted by admin on Jun 30, 2022 in |
The incidence of multiple antibiotic-resistant Salmonella spp. isolates in randomly collected potable water samples from 70 different slum area sites of Bhopal City, M. P. India, were investigated through a molecular approach.BSA media was used for isolation and CFU count of Salmonella spp. The DNA of representative Salmonella spp. Colors of each water sample were subjected to PCR to detect Amp, cmlA and tetA genes with standard PCR protocol with annealing at primers specific temperature for 1 minute 30 seconds. The presence of the target gene in the PCR product was detected on 1% agarose gel. An in-vitro antibiogram study was done to check the phenotypic antibiotic resistance. A total 74.29% of samples were reported to have a prevalence of Salmonella spp. with 44.19average CFU count and huge standard deviation (σ=28.28). Incidence of Amp, cmlA, and tetA genes is reported to be 67.31%, 36.54%, and 61.54% in Salmonella spp. Isolates from 52 samples which doesn’t coincide with the phenotypic prevalence of antibiotic resistance. 25, 17, and 11isolates showed resistance...
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Posted by admin on Jun 30, 2022 in |
Anxiety is one of the main psychiatric disorders worldwide. Furthermore, it is difficult to find out the patients suffering from various kinds of anxiety diseases. Numerous herbal plants have been utilized to treat the anxiety disease in traditional medical systems for thousands of years because of their safety, efficacy, and effectiveness. The present study was designed to evaluate the anti-anxiety activity of the hydroalcoholic extracts of the leaves of the Achyranthes aspera plant in rodents like mice. The anti-anxiety activity was assessed in swiss albino mice. The leaf of the Achyranthes aspera plant was air-dried and was reduced to coarse powders. The Soxhlet extraction method was used for this anti-anxiety activity. Qualitative phytochemical screening of hydroalcoholic extracts of Achyranthes aspera was carried out. Several models were used to evaluate the anti-anxiety activity of the leaves of the Achyranthes aspera plant: the elevated plus-maze model, the light-dark model, the social interaction model, and the hole board technique. For this, the dose of hydroalcoholic extract p.o. at 300 and 600 mg/kg,...
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Posted by admin on Jun 30, 2022 in |
Furan and Azetidinone have been reported to possess various therapeutic activities, including anti-microbial and anti-cancer activity. Increasing drug resistance and high toxicity to the existing drugs have dictated the need to develop novel, effective agents with different scaffolds. With the perspective of identifying novel anti-cancer molecules, an approach was made to hybridize the different heterocyclic moieties. Thirtynovel Furan-Azetidinone hybrids were subjected to in-silico molecular docking studies to determine the novel molecules’ affinity for the anti-cancer targets. The four most promising derivatives were selected and synthesized based on docking scores. Condensation of ethyl cyanoacetate and o-toluidine at 160-1900C yielded 2-cyano-N-2(methylphenyl) acetamide (1). The reaction between (1) and benzoin yielded 2-amino-N-(2-methylphenyl) 4, 5-diphenylfuran-3- carboxamide (2). A series of Schiff bases ARJJ03 (1-2), were synthesized from (2) by refluxing it with various substituted aromatic aldehydes in ethanol using concentrated sulphuric acid as the catalyst. The title compounds ARJJ04 (1-2) were obtained by the cyclization of ARJJ03 (1-2) with Chloroacetyl chloride in 1, 4-dioxane in the presence of triethylamine. The synthesized derivatives were...
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Posted by admin on Jun 30, 2022 in |
Candesartan cilexetil is widely used for the treatment of hypertension. Candesartan cilexetil is a pro-drug administered orally, rapidly converted to its active metabolite candesartan during absorption in the gastrointestinal tract. Candesartan cilexetil is a BCS II drug that is practically insoluble in the water of 0.00771 mg/ml & it has a low dissolution rate, hence; to improve dissolution rate & bioavailability, solid dispersion of Candesartan cilexetil were prepared by kneading method, solvent evaporation method & Microwave method in 1:0.5, 1:1 & 1:1.5 ratios of Candesartan cilexetil and hydroxy propyl beta-cyclodextrin /PVP K 30. Further, the SDs were filled into empty hard gelatin capsules with excipients like starch (8%), lactose, talc & Aerosil by manual capsule filling method. The formulated solid dispersions were evaluated for drug content and in-vitro dissolution studies. Accelerated stability studies was conducted for the optimized SD formulations, the optimized drug & carrier SD were confirmed & characterized by FT-IR. The drug content of the prepared Candesartan cilexetil SD formulations was found to be range from 94.35±0.95%...
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Posted by admin on Jun 30, 2022 in |
Ornamental plants (OPs) are used for intensifying, glorifying surroundings, adding a positive attitude towards life, enhancing estate value, and developing a feeling of well-being and happiness. This research aimed to record the ornamental plants and ethnomedicinal document usage of the plants by the local people of the Rewari district of Haryana State. The data was collected through field surveys and in-depth interviews organized with 219 local informants from the region during 2019-21. A total of 80 different locations were visited during the study. 91 OPs belonging to 83 genera and 42 families were identified and documented from the Rewari district. The documented plants showed a diversity of habit ranging from herbs (43.96%), trees (24.18%), shrubs (24.18%), twines (3.30%), and climbers (3.30%) and creepers (1.10%). The majority of ornamental plants were utilized as garden plants (25.50%) followed by pot (25.19%), bouquet/ cut flower/ cut foliose (11.41%), avenue (9.44%) road dividers (7.87%), religious and cultural (7.87%). Families Leguminosae (11.95%) and Asteraceae (7.60%) were the dominant families. Maximum no. of ethnomedicinal uses...
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