Posted by admin on Feb 28, 2022 in |
Objective: To develop a simple, selective, and precise stability-indicating high-performance liquid chromatography method for the simultaneous estimation of acebrophylline and doxofylline in bulk and tablet dosage form. Methods: The chromatographic separation achieved on HiQSil C18 Column (250 × 4.6 mm, 5µm) utilizing a mobile phase Acetonitrile: 10 mM n-hexane sulfonic acid buffer (80: 20, v/v) at a flow rate of 1.0 ml/min with injection volume 20 μl. UV detection was performed at 250 nm. The method was validated as per ICH guidelines. Results: The retention time for acebrophylline and doxofylline was found to be 2.77 min and 9.56 min, respectively. The linear regression analysis data for the calibration plots showed a good linear relationship in the concentration range of 1-10 μg/ml for acebrophylline and 4-24 µg/ml for doxofylline. The percentage recoveries of acebrophylline and doxofylline in the marketed dosage form were found to be 99.91 and 94.24, respectively. The correlation coefficients for acebrophylline and doxofylline were 0.997 and 0.998, respectively. The percentage degradation at different stress conditions like acid,...
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Posted by admin on Feb 28, 2022 in |
Studies on dextrocardia have been limited by low numbers. Hence, finding the most common diagnosis in patients with dextrocardia and situs ambiguus is challenging among patients seeking medical attention in a tertiary care center. Aims and objectives: To identify the most common diagnostic pattern in dextrocardia (DC) patients with situs ambiguous (SA). Methods: It is a retrospective study with records dating backto the last 21yrs from a major tertiary care center in South India. In this study, researchers will include all the patients diagnosed with dextrocardia (defined as the right-sided baso-apical axis of the heart). The segmental analysis will be done as described previously. Results: There was a total of n=378 patients with dextrocardia, of which 71 had situs ambiguus with dextrocardia. The mean age was 2.3yrs, 36.6% were females 32 patients had right atrial isomerism (RAI), 18 patients had left atrial isomerism (LAI), and 21 patients had unidentified atrial isomerism (UAI). In the RAI group Double outlet right ventricle (DORV) was present in n=13 (40.6%), Single ventricle (SV)...
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Posted by admin on Feb 28, 2022 in |
The objective of the present study was to develop cyclodextrin-based nanosponge based topical gel of tazoretene using cross-linker diphenylcarbonate and Carbopol® Ultrez 10 NF polymer. The β-Cyclodextrin nanosponges were prepared using various concentrations of β-Cyclodextrin and diphenyl carbonate and characterized. Based on evaluation parameters the β-Cyclodextrin nanosponges formulations (NS2) displayed narrow particle size, sufficiently high particle size, and maximum solubilization efficiency. Tazarotene was loaded into five β-Cyclodextrin nanosponge formulations by freeze-drying method and evaluated. Owing to the better solubilization and drug loading capacity (45%) TZNS3 was selected for further studies. The particle size of 336 nm, the zeta potential of -23 mV, and maximum drug dissolution of 97%in 12h were displayed by TZNS3 hence formulated into a gel and evaluate. The optimized tazarotene-loaded nanosponge formulation (TZNS3) was incorporated into a model carbopol gel formulation and was evaluated for skin permeation and stability. The flux value for nanosponges based gel formulation (189.342 ± 3.879 μg cm−2 h−1) was found to be higher than that for plain tazarotene (106.765 ± 4.123...
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Posted by admin on Feb 28, 2022 in |
Doravirine is an HIV-1 non-nucleoside reverse transcriptase inhibitor (NNRTI). The aim of the present investigation was to develop a self-nanoemulsifying drug delivery system (SNEDDS) to enhance the solubility and dissolution of poorly water-soluble doravirine. The solubility of doravirinein various oils was determined to identify the oil phase of SNEDDS. Various surfactants and co-surfactants were screened for their ability to emulsify the selected oil. A ternary phase diagram was constructed to identify the efficient self-emulsifying region. The optimized SNEDDS formulation (F8) contained drug (100 mg), neobee M5 (22.2%), caproic acid (58.2%) and PEG 600 (19.4%). The SNEDDS was further evaluated for its robustness, turbidity, % drug content, entrapment efficiency, droplet size, and zeta potential. The optimized formulation of drug-loaded SNEDDS exhibited 98% entrapment efficiency, 99% drug content and 99% in-vitro drug release in 60 min as compared with the plain drug, which had a limited dissolution rate (31%). The particle size for the optimized formulation of SNEDDS (F8) was found to be 67.8 nm with PDI 0.173. The negative value...
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Posted by admin on Feb 28, 2022 in |
Background: Satranidazole is a class II drug per biopharmaceutical classification systems with poor aqueous solubility and antiprotozoal and anti-bacterial properties. Aim: Therefore, the purpose of the present study was to enhance the solubility of Satranidazole using a self-emulsified drug delivery system (SEDDS). Methods: SEDDS was prepared using Satranidazole (10 mg) and dissolved in the oil phase, i.e., rice bran oil and GMO (1:9). The co-surfactant, propionic acid, was added to the oil phase, and then Tween 80 was added with continuous stirring. The mixture was allowed to homogenize at 40 °C for 24 h and was stored in a stoppered glass vial until further evaluation. The total amount of the mixture was kept constant at 0.37 ml to be filled in size 2 capsules for dissolution study. Physico-Chemical Evaluation: Four SEDDS formulations were prepared (F-1 to F-4) and evaluated for droplet size analysis, stability studies, zeta potential, drug content and in-vitro drug release. Results: Based on self-emulsification time & dispersibility, droplet size analysis, drug content and in vitro drug...
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