Posted by admin on Jun 30, 2022 in |
In the present research work, a new UV spectroscopic and RP-HPLC method was described for simultaneous estimation of Aceclofenac (ACL) and Pantoprazole (PNT). Simultaneous Equation method was applied for the spectrophotometric determination of ACL and PNT. Statistical calculations were done by use of 276 nm and 289 nm wavelengths. In RP-HPLC method, the estimation of ACL and PNT carried out by ACN: Buffer (Phosphate acetate) (80:20 v/v) as mobile phase, pH 6.8 at a flow rate of 1.0 mL/min and YMC Triart C18 column (250 mm × 4.6 mm, 5 µ), column. The detection of ACL and PNT was carried out at 295 nm. The retention time of ACL and PNT was found at 3.739 and 2.991, respectively. The results of analysis in both the methods were validated as per ICH guidelines in terms of linearity, range, accuracy, precision, LOD, LOQ, ruggedness, robustness, and solution stability; from this study, it was concluded that the developed method of RP-HPLC can be successfully used for the estimation of ACL and PNT...
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Posted by admin on Jun 30, 2022 in |
Artemisia annua L. is an herbaceous plant from which the antimalarial sesquiterpene lactone, Artemisinin, is obtained. In Ghana, Artemisinin-Combination therapies are used as first-line treatment for managing malaria. The present study, the first of its kind in Ghana, sought to carry out the preliminary morphological and phytochemical evaluation and investigate the artemisinin content of Artemisia annua planted in Ghana. Seeds of A. annua were planted, and the dimensions of the shoots of the germinated seedlings were taken from week one till maturity. Qualitative phytochemical analysis was performed on the powdered plant parts following standard techniques. The artemisinin contents of the leaves and stem were determined using Ultra-High-Performance Liquid Chromatography coupled with a triple Quadrupole Mass Spectrometry (UPLC-QqQ-MS) using Multiple Reaction Monitoring (MRM) modes. Morphological observation showed that the Ghanaian grown A. Annua attained an average height of 36.94 cm at maturity. The leaves are lobed and have a strong aromatic odour. The ethanol and petroleum ether extracts of leaves, stem and roots s tested positive for secondary metabolites such...
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Posted by admin on Jun 30, 2022 in |
Objective: A simple, rapid, accurate, precise and reproducible validated RP- HPLC method was developed for the determination of Lecithin and Silymarin in bulk and tablet dosage forms. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, the limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. Results: The quantification was carried out using Symmetry PremsilC18 (250mm x 4.6mm, 5µm) column run in an isocratic way using mobile phase comprising of acetonitrile: water (0.1% OPA with PH 3) 70:30 v/v and a detection wavelength of 236 nm and injection volume of 20µL, with a flow rate of 1ml/min. The retention times of Lecithin and Silymarin were found to be 6.58 min and 3.616min. The method was validated in terms of linearity, precision, accuracy, LOD, LOQ, and robustness in accordance with ICH guidelines. The assay of the proposed method was found to be 99-101%. The recovery studies were also carried out, and the mean %...
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Posted by admin on Jun 30, 2022 in |
To compare the effect of combined oral contraceptive (COC) and progesterone-only injectable contraceptive (PIC) on the inflammation, we recruited 47 female participants (16 to 43 years old) in 3 groups: control participants (n=10), COC users (n=16) and PIC users (n=21). Using ELISA and Latex agglutination methods, we measured the effect of hormonal contraceptives on the inflammatory blood biomarker, C-reactive protein (CRP). Data were collected from July 2019 to January 2020 from Rajshahi Medical College and Hospital. The users of both COC and PIC had higher levels of CRP (p<0.001), as compared to the controls, and COC users had higher levels as compared to PIC users (p<0.001). We also found a correlation between the CRP data of contraceptive users and some socio-demographic variables. The CRP values of different demographic variables in the Elisa method but not in the Latex method were significantly correlated (p<0.01). There was a significant association between the types of contraceptives used, as measured both Latex and Elisa methods. Our data suggested that limiting hormonal contraceptives can...
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Posted by admin on Jun 30, 2022 in |
Adenosine release is more apparent after seizures or in-vitro seizure-like activity and tissue adenosine level increases quickly after seizure commencement. These adenosine increases linked to seizures are reflections in composing a response mechanism that controls seizure intensity and duration. Much evidence from animal studies suggests that this impact is mediated by the adenosine receptor (AR), just as it is with neurotransmission in general. Efforts to produce centrally acting treatments and reduce peripheral side effects, especially cardiac adverse effects, have long been prioritized in drug development. New study is targeting highly precise areas of the A2R might be a step forward in direct A2R-based anti-seizure therapy. Despite significant attempts to introduce innovative tactics for the treatment of various kinds of epilepsy, this illness continues to be a serious problem across the globe. Taking inspiration from the facts above and recognizing the growing need for anti-epileptic compounds with improved pharmacodynamics and pharmacokinetic profiles in the worldwide market. The present in-silico research involves studying the anti-convulsant (anti-epileptic) potentials of some substituted 2′-(prop-1-en-2-yl)-1′,2′,3′,4′-tetrahydro-[1,1′-biphenyl]-2,6-diol...
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