Posted by admin on Dec 31, 2021 in |
In the present work, simultaneous estimation of Hydrochlorothiazide and Methyldopa was carried out by applying chemometric assisted UV spectro-photometric methods. The Two chemometric methods, classical least square (CLS) and inverse least square (ILS) were applied for estimation of Hydrochlorothiazide and Methyldopa simultaneously in tablets without any prior separation, and no graphical treatment of the overlapping spectra of these drugs were required. The chemometric calculations for CLS and ILS methods were performed by using the Chemometrics Toolbox 3.02 software equipped with MATLAB 6 and excel. The results derived from these two chemometric methods were compared statistically. These two chemometric methods were successfully applied estimation of two drugs from their marketed tablet dosage forms without any prior separation procedure. The methods were validated according to ICH guidelines. The results of the recovery study were found to be closer to 100 percent, and relative standard deviation obtained for different parameters of ILS and CLS methods was found to be within a limit for both the drugs. The two chemometric methods mentioned in...
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Posted by admin on Dec 31, 2021 in |
Many species of the genus Selaginella P. Bauev. (family: Selaginellaceae) are used as traditional medicine. In India, S. involvens (Sw.) Spring and S. bryopteris (L) Bak are used for medical purposes. In some works, these two species have been treated as resurrection plants or ‘Sanjeevani’. These plants have considerable morphological similarity which can create confusion regarding the exact plant used as medicine by traditional practitioners. The present study focuses on the comparison of both species for confirming their identity as two distinct species and their potential as medicinal plants. They were compared for morphological and anatomical characters, megaspore ornamentation (SEM analysis) and phytochemical analysis (HPTLC of bioflavonoids, amentoflavone). Morphologically S. bryopteris appears to be a miniature form of S. involvens. Anatomically they differ in the stelar region with differences in the position and number of protoxylem groups and the shape of the vascular region. The general appearance of the megaspore and ornamentation of the spore wall had significant differences. A prominent difference was observed in the sizes of their...
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Posted by admin on Dec 31, 2021 in |
The purposes assigned to this work are the phytochemical screening of certain phytoconstituents, the estimation of the antioxidant and antibacterial activities of extracts of Azadirachta indica A. Juss. The selective extracts obtained from the aqueous decocts were used to establish the chromatographic profile of the various extracts. As for the crude aqueous extracts, they were used to carry out the tests by color reactions to evaluate their antioxidant potential as well as their antibacterial activity against 12 strains. Phytochemical screening revealed the existence of a few bio-important phytoconstituents. The median effective reduction concentration (CR50 = 0.10 mg / mL) demonstrated the antioxidant efficacy of trunk bark decocted (ET) vis-à-vis DPPH, compared with vitamin C (CR50 = 0.006 mg / mL), the reference antioxidant. Also, the extracts, in particular ET, exhibited almost bactericidal activity against the strains tested with the exception of Pseudomonas aeruginosa 469 UB / 20 CNRa. Both the antioxidant and the antibacterial activity of the plant vary depending on the type of extract, organ and bacterial...
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Posted by admin on Dec 31, 2021 in |
A model of ascending Urinary tract infection (UTI) in rats was developed to study the significance of antibacterial activity of Sphaeranthus indicus L. and compared with standard antibiotic ofloxacin as a positive control. This plant used in folk medicine as a remedy for urinary tract infections is belong to Asteraceae family. Outbred female wistar rats were used throughout the study and Escherichia coli was used as pathogen isolated from the urine of patients suffering from urinary tract infections. Methanolic extract (300 mg/mL) and aqueous extract (300 mg/mL) of Sphaeranthus indicus were administered orally for 15 days. After 15 days of treatment of the infected rats with herb extract, bacterial count in the urine, immunoglobulin G and M levels were estimated, and his pathological changes of kidney, liver, and urinary tract were observed. In the disease control group bacterial infection has increased than that of the normal control group. In the treated group with aqueous extract (300 mg/mL) & methanolic extract (300 mg/mL) bacterial count has decreased considerably. There was...
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Posted by admin on Dec 31, 2021 in |
Introduction: Femur is the key bone among the skeletal remains to determine sex for anatomists, anthropologists, and forensic experts. In the present study, we considered most of the parameters to determine sex from the femur. Methods: We studied eighty-four undamaged, intact femora of unknown sex. The present study were carried out in S. C. B. Medical College Cuttack from December 2019 to April 2020. The parameters, like maximum length, maximum breadth, head circumference, mid-shaft circumference, and vertical diameter femur head, were measured by a goniometer and verniers caliper. Results: From each femur above-mentioned parameters were recorded, and the details were obtained about the mean, Standard Deviation, percentage, and p-Value. Then these details were compared with the findings of other studies to conclude. Conclusion: These measurements show the values are higher in males than females and were statistically highly significant between males and females with a p-value...
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