Posted by admin on Mar 31, 2022 in |
Pullulanase (Pullulanase-α-glucanohydrolase EC 3.2.1.41) is a glucanohydrolase enzyme that can break down the pullulan and allied oligosaccharides into glucose. Promising of starch-based industries are biofuels, starch syrup, nutraceuticals, etc., increase the glucanohydrolases demand. Present investigation revealing that the Bacillus cereus KKSJ 1981 is enhanced the production of pullulanase through the optimization of temperature, agitation speed, pH, soluble starch concentration, MnSO4 and yeast extract. Response surface methodology (RSM) was utilized to optimize the selected six parameters. A central composite design with 50 experiments was performed in this investigation. The analyzed data depicts that the correlation coefficient (R2) value was 0.9873, and the adjusted R2 value was 0.9717 indicates the high significance of the model. Further, the selected parameters in optimum conditions were predicted through the developed mathematical model, and validation experiments were performed at these conditions. Overall, 1.2 folds of pullulanase were improved through the RSM based...
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Posted by admin on Mar 31, 2022 in |
The research work is focused on the formulation of dermal drug delivery system of Fisetin. The formulation based on the principal to causes deeper skin penetration and minimal systemic delivery for the management of skin cancer by enhancing the dermal bioavailability of formulation with reducing dosage requirement in sustained delivery mode by direct targeting of formulations to the skin. Two formulations viz transethosome and ethsosme were formulated to attain the efficient dermal delivery of fisetin. The surface morphology was studied by using Zeta sizer procedures. The formulation had shown adequate strength, consistency, cohesiveness, and list of thickness as 330.50 g, 314.69 g, 2.34.50 g, and 112.71 g separately. The streamlined fisetin transethosome gel definition detailing had 90% spreadability and a pH of 6.5 ± 0.2. These properties guarantee uniform spreading of OFTF-gel at the application site without causing skin irritancy. OFTF-gel had shown great substance consistency with 99.5 ±1.5 % of medication recuperation from the gel by HPLC assessment. The skin treated with transethosomes gel had shown altogether higher...
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Posted by admin on Mar 31, 2022 in |
In the present study, the aqueous and ethanolic extracts of Vitis vinifera seeds were studied for alpha-amylase and alpha-glucosidase inhibition using an in-vitro model. The plant extracts were also examined for their antioxidant activities by using the free radical 1, 1-diphenyl-2-picryl hydrozyl (DPPH) scavenging method under in-vitro. The study revealed that the different concentrations of the extract possessed a very good amount of result and exhibited potent radical scavenging activity using DPPH SA substrate. The aqueous (68.5%) and ethanolic extract (74.2%) of Vitis vinifera seeds showed a significant inhibitory effect on alpha-amylase activity ethanol extract (74.2%), aqueous extract (68.5%). In the alpha-glucosidase activity ethanolic extract (75.7%) and aqueous extract (74.5%) showed a significant inhibitory effect when compared with standard acarbose drugs. Therefore, it is suggested that the aqueous and ethanolic extract of Vitis vinifera seeds could be used as a potential source for natural anti-diabetic and antioxidant compounds and could have the potential use in the management of diabetes mellitus and...
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Posted by admin on Mar 31, 2022 in |
Copper is an essential trace element involved in a plethora of functions being a component of several enzymes. Copper deficiency affects people of all age groups, including infants, children, pregnant women, and men. Deficiency occurs due to numerous factors hindering the absorption/uptake of copper. Male Wistar rats (35-50 gm) were divided into four groups: Group 1: Negative control – fed with standard feed (Ashirwad Industries, Chandigarh) and tap water was provided ad libitum .Group 2: Copper control group- Fed with diet containing 126 nmol Cu/gm. Tap water was provided ad libitum. (c) Group 3: Pair-fed group – Fed with 126 nmol Cu /gm diet but the amount of feed given was equal to the feed consumed by the copper-deficient group the previous day to account for stress and starvation effect caused due to low intake diet. Tap water was provided ad libitum (d) Group 4: Copper deficient- animals were fed with 6.3 nmol Cu / gm diet, and demineralized water was provided ad libitum. The experiment was conducted for...
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Posted by admin on Mar 31, 2022 in |
Ondansetron hydrochloride (ONH) is an exceptionally harsh, powerful antiemetic drug utilized for the treatment and prophylaxis of chemotherapy or radiotherapy or postoperative prompted emesis. This investigation aims to detail and assesses taste-covered quick-dissolving tablets (FDTs) of ONH to expand patient consistence. ONH taste veiled granules were set up by a strong scattering procedure. Dissolvable dissipation techniques were utilized for such readiness. Totally taste veiling with zero arrival of medication in phosphate support pH 6.8 was acquired from granules arranged by dissolvable vanishing strategy utilizing drug: polymer proportion of 1:2, from which four equations pass a pre-pressure assessment and compacted to FDTs and assessed for their medication content, in-vitro breaking downtime, in-vivo deterioration time, wetting time and in-vitro drug discharge profile. It was tracked down that the tablet definitions F8 with cross carmellose sodium as super disintegrant showed the fast medication discharge when contrasted with unadulterated medication. T50 and DE 30% for the definition F8 was discovered to be in 7 min and 73.5%, Hence reasonable as quick-dissolving...
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