Posted by admin on Jan 31, 2022 in |
Human beings always tried to protect their body from pathogenic microbes, whereas these pathogens always mimic and mutate themselves to infect humans. Nanotechnology is new and upgrading itself as well as enhancing its applicability in pharmaceuticals. Due to their nano-size structure, property drastically changes from their parent elements. Nanoparticles are used widely in biomedical sciences. Focusing on these perspectives, the present study investigates the impact of phyto-synthesized Zinc Oxide Nanoparticles (ZnO NPs) using Murraya paniculata leaf extracts. The characterization of synthesized ZnO NPs was 32 nm with a more optical stable showing peak value at 355 nm (UV-vis). Further, toxicological efficacy of ZnO NPs was checked against human pathogens viz., Salmonella typhimurium (causing typhoid), Staphylococcus aureus (causing tooth decay and rheumatic fever) and Escherichia coli (causing tooth decay in infants and dysentery). The anti-bacterial bioassay was done using a well diffusion method and compared with the standard drug streptomycin. The test pathogens were found more sensitive towards phyto- synthesized ZnO NPs with a Zone of Inhibition Diameter (ZID) value...
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Posted by admin on Jan 31, 2022 in |
The present research effort was undertaken to isolate phytoconstituents from pharmacologically active extracts of stem bark of Albizia stipulata based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of bark of Albizia stipulata were prepared using various solvents such as methanol, hydro alcohol, and water. These extracts were screened for in-vitro pharmacological activities like antioxidant, anti-inflammatory, and anti-diabetic activities. The active extract was subjected to column chromatography through a mixture of solvents to get fractions and eluted fractions were run in TLC using various mobile phases with different solvent ratios. The isolated compounds were subjected to IR, 1H NMR, 13CNMR and LC-MS spectral analysis for chemical characterization. Results: The methanol extract of bark of Albizia stipulata was potent compared to other extracts. This extract was subjected to column chromatography to get fractions, and eluted fractions were run in TLC. The fractions with similar Rf values to standard were united and crystallized. The spectral analysis confirmed that the isolated compounds were found to be β-Sitosteryl-3-O-β-D-glucoside and 3...
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Posted by admin on Jan 31, 2022 in |
In this study, Raman spectroscopy was used as an analytical quality control methodology to evaluate the paracetamol (PCT) quantity in a commercially available formulation in the pharmaceutical industry. The Raman Spectroscopy has been established for the validation and the quantification of PCT in a commercial product called Paracerol (Prc) Solution for I.V. Infusion containing 10.0 mg.mL-1 PCT. The validation of the proposed method was performed by evaluating the specificity, linearity range, accuracy, precision, detection limit, quantification limit, and robustness parameters. The acceptable results for all parameters showed that the proposed method is valid. The Raman spectroscopic method was also compared with the HPLC method in terms of PCT determination and statistical tests such as t and F tests and the obtained results indicated that there is no any difference in the averages and precision results between the proposed Raman spectroscopy and HPLC methods. It has been reported that Raman spectroscopy can be applied in the pharmaceutical industry for the quality control analysis of the commercial formulation of...
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Posted by admin on Jan 31, 2022 in |
Current analytical research and experiments provide the development of a simple, sensitive, accurate, rapid, and stability indicative RP-UPLC method for estimation of Levocetirizine in Levocetirizine dihydrochloride tablets. This development was achieved using a Waters Acquity UPLC with BEH, C18 column of dimensions of 100 mm × 2.1 mm, 1.7 µm column at 0.3 mL/min flow rate and Acquity TUV detector at 230 nm. This method was validated based on the guidelines of the International Council on Harmonization (ICH). Linearity was demonstrated at 25% to 150% levels with R2 value of 0.999. Precision and accuracy were in line with the ICH guidance with a mean recovery of 99.02%. The RP-UPLC method is sensitive with levels of Limit of detection (LOD) and Limit of Quantitation (LOQ) at 0.04µg/mL and 0.12µg/mL, respectively. Degradation studies in conditions of Oxidation, Acid, Base, Temperature, Water, UV light demonstrate there is no placebo as well as degradation impurities interfering with the Levocetirizine main peak. The simple, sensitive, accurate, rapid, and stability-indicating method makes it an efficient...
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Posted by admin on Jan 31, 2022 in |
Multi particulate systems are of greater importance than the single unit dosage forms in oral drug delivery. Floating micro balloons are among the best methods to increase gastric retention among all the multi particulate drug delivery systems. They offer more reproducible drug absorption, reduce the risk of local irritation and improve the bioavailability of the drug. In the current research work, clopidogrel, a BCS class – II drug, was formulated as controlled release micro balloons using ethyl cellulose as polymer and span 80 as the surfactant. The pre-formulation studies viz. solubility, partition coefficient, micromeritics were carried for the Clopidogrel bisulphate pure drug, and the drug excipient compatibility studies were carried using Fourier transform infrared spectrophotometer and Differential Scanning Calorimeter. Different formulations of floating micro balloons were prepared by solvent evaporation method by altering five process and formulation factors viz. concentration of surfactant, the volume of solvent, volume of internal phase, the concentration of polymer, speed of rotation. All the Clopidogrel micro balloons were subjected to in-vitro drug release and...
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