Posted by admin on Sep 30, 2021 in |
The current research paper describes a highly specific, reproducible, and efficient stability-indicating HPTLC method for estimation of Celecoxib and Amlodipine Besylate from its synthetic mixture. Chromatographic separation and quantification carried out on Merck HPTLC aluminum sheets of silica gel 60 F254 using Chloroform: Acetone: Toluene: Methanol in the ratio of 5:3:2:0.5 v/v/v/v as solvent system. This system was found to give compact and dense spots for CXB and AML with the Rf value of 0.80, 0.23 respectively. Densitometric analysis of CXB and AML was done at 238 nm and 366 nm. Regression analysis for the calibration plots was indicative of good linearity between response and concentration over the range of 0.2-1.2 µg/spot for AML and 4-24 µg/spot for CXB. Forced degradation studies were performed under different conditions. Both drugs were degraded in acidic, basic, oxidative, thermal, and photolytic conditions. In the present research, a stability-indicating HPTLC method has been developed for Celecoxib and Amlodipine Besylate. The developed method was validated as per ICHQ2R1 guidelines and was successfully applied for...
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Posted by admin on Sep 30, 2021 in |
The present study of concurrent process validation delivers an extraordinary degree of quality assurance that a specific process for manufacturing of Glibenclamide Tablets will consistently manufacture a product that meets its predetermined quality attributes and specifications. Glibenclamide is generally suggested for the treatment of type II diabetes mellitus and it is mainly a sulfonylurea derivative. It mainly comprises the stages to be followed to evaluate and qualify the acceptability of the manufacturing process of Glibenclamide 2.5 mg tablets. The process is limited to the three batches H, I, and J manufactured of specific batch size with the help of specified equipment’s and different quality control parameters for tablets. It involves all parameters related to each step were evaluated by the respective standard test involved in the manufacturing. All analytical results of each stage were found to be within the acceptable limit and criteria. Other tests related to compression such as hardness, thickness, disintegration and dissolution for all three batches were also found within the acceptable...
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Posted by admin on Sep 30, 2021 in |
Piperazine is a nitrogen-containing heterocyclic compound and has fascinating applications in drug discovery and development. In the present study, novel 4-(benzo[1,3]dioxol-5-ylmethyl) piperazine amide derivative was prepared by acid amine coupling of 1-piperonyl piperazine derivative and benzoic acid. Synthesized compound was characterized by IR, NMR and mass spectral studies. Further, in-vitro cytotoxic effects were carried out in MTT in various human cell lines HeLa, MCF7, MDA-MB-231, HCT116, and HT29. Out of these diverse cell lines, compound 3 showed promising cytotoxic effects on MDA-MB-231 with the IC50 estimation of 11.3 µM. The surface morphology by Colonogenic assay and in-vitro cell migration assay measures better movement on MDA-MB-231 in compound 3. Further, apoptotic-related examination under AO/Eb staining, commet assay, and cell cycle investigation exhibited that compound 3 demonstrated significant activities on MDA-MB-231 by activates apoptosis and blocking cell cycle moment in the G0/G1 stage. All the more altogether, staining the cells in the meantime with Annexin V-FITC/PI. The synthesized compound 3 indicated tremens-dous repressing capacities in cell cycle and activates apoptosis because...
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Posted by admin on Sep 30, 2021 in |
Natural phenolic acids alkyl esters viz methyl, ethyl, propyl was synthesized and characterized by spectral means FTIR, 1HNMR, and 13CNMR. All the synthesized esters were examined for their antimicrobial potential, preservative efficacy and antioxidant potential. Among the synthesized ester derivative caffeic acid and gallic acid, derivatives were displayed excellent antioxidant and antimicrobial potential. They were further evaluated their preservative efficacy according to USP 2004 protocol for preservative effectiveness testing. Caffeic acid propyl ester and gallic acid ethyl ester exhibited promising preservative potential better than existing preservative agents. From the study, we can conclude that these caffeic and gallic acid derivatives can be used as lead compounds to further explore their application as preservative agents in pharmaceuticals and in the food...
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Posted by admin on Sep 30, 2021 in |
Many herbs/phytochemicals are proved to have anti-diabetic properties and patients in the treatment of diabetes increased their usage. Administration of herbs and allopathic drugs has a chance of interaction between them, leading to alterations in pharmacokinetics and pharmacodynamics of the drug. The present study was aimed to know the influence of phytoconstituent (Allicin) on the pharmacokinetic and pharmacodynamic parameters of the drug. Pioglitazone is a thiazolidinedione class of oral anti-diabetic drug which was metabolized by CYP enzymes. The combination of piglitazone with allicin, increases the AUC in normal and diabetic rats. The pharmacodynamic parameter like blood glucose levels of diabetic rats was decreased due to the increase in the bioavailability of the drug. Allicin shows inhibitory action on CYP enzymes and decreases the metabolism of pioglitazone. However, the combination of pioglitazone with allicin alters the metabolism of the drug in diabetic rats and doses of herb-drug were monitored...
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