Posted by admin on Sep 30, 2021 in |
This study assessed the biological properties and safety profile of extracts from locally grown banana leaves in Arusha (Tanzania), to affirm their possible use for wound dressing. Screening for phytoconstituents in extracts from studied banana plant species, ijuhi inkundu (IJ) mlelembo (ML) and kimalindi (KIM), revealed the presence of various secondary metabolites; anthraquinones, alkaloids, flavonoids, tannins, terpenoids, phenols, phytosterol and saponins. Susceptibility of microorganisms to studied banana varieties were in the order of KIM > ML > IJ. One-way analysis of variance (ANOVA) revealed a statistical difference of mean among all extracts (p < 0.05). Antioxidant activity was evaluated by measuring the ability of extracts to scavenge DPPH free radicals. Scavenging of DPPH was in the order of > kimalindi > ijuhi inkundu > mlelembo. Brine shrimp results for toxicity showed that almost all extracts were non-toxic to the shrimps, exhibiting mild toxicity by giving the LC50 values higher than 100...
Read More
Posted by admin on Sep 30, 2021 in |
To evaluate the antibacterial activity of methanolic extract of Terminalia chebula, commonly called Haritaki was tested against respiratory tract infection-causing bacteria. The antibacterial activities were assessed by agar well diffusion, broth dilution and time-kill methods. It showed the best antibacterial activity against Klebsiella pneumoniae among the tested respiratory infection-causing bacteria (Streptococcus pneumonia and Streptococcus pyogenes) in the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), at 100 mg/ml, respectively. Killing ability depends on the time and concentration of the extract, which was found optimum at 20 h at 100 mg/ml. The cell viability and cytotoxicity of the extract were tested on swine Lung Cell lines with different concentrations (100-500 mg/ml). The viability of the cells was more at 100 mg/ml, whereas the viability gradually decreases by increasing the concentration was determined by 3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay. The extract showed minimal hemolytic effect in human red blood cells in Biocompatibility assay at 100 mg/ml, which was tested against standard drug levo flaxacin. It suggests that the methanolic...
Read More
Posted by admin on Sep 30, 2021 in |
Diabetes mellitus is a metabolic condition characterized by insulin resistance, or poor secretion, or both. There are several side effects associated with drugs available to cure diabetes. The most common side effect is neurodegenerative. The objective of the present research was to evaluate the efficacy of Cuminum cyminum seed against diabetes and also to evaluate its nephroprotective power in the diabetes mice model. Twelve mice were housed, caged, and segregated into three groups for 28 days (four weeks). Control, diabetic control, and diabetic with Cuminium seed treatment. The diabetic mice model was prepared by a single dose of alloxan (110 mg/kg/bw) introduced intraperitoneally. Glucose monitoring and diabetic condition checked and confirmed by a one-touch glucometer. Alcoholic extract of Cuminum cyminium seed (80mg/kg/ body weight) orally administrated to the diabetic group of mice up to 28 days. After the completion of the whole experiment, animals were sacrificed blood samples were collected for the investigation of biochemical parameters, including glucose, urea, uric acid, and creatinine. There were the least changes observed...
Read More
Posted by admin on Sep 30, 2021 in |
Aim: The present study focuses on studying the soft rot-causing bacteria in Solanum tuberosum and the activity of phytoextracts against the pathogen to prevent/control this infection. Lantana camara and Clerodendrum infortunatum has been well known for their antibacterial activity and widely used in Ayurveda, these plants were investigated for their antibacterial activity against the isolated pathogen. Methods: The bacteria were isolated from the infected potato sample and cultured in Potato dextrose agar (PDA) media. The isolated was characterized using morphological/biochemical properties and 16S rDNA sequencing. The pathogen was subjected to agar well diffusion assay to investigate antibacterial potential of L. camara and C. infortunatum extracts against this pathogen. Results: A gram-negative cocci were isolated from the infected S. tuberosum sample. Phylogenetic analysis using 16S rDNA sequencing, suggested no previous reports of such bacteria causing soft rot of potato. The 16S rDNA sequence was submitted to GenBank with accession ID MT491092. Phytoextracts of L. camara demonstrated significant antibacterial activity against the isolated bacterial strain SJCTSSP01. Prominent results were observed from...
Read More
Posted by admin on Sep 30, 2021 in |
Cow urine is considered a Sanjeevani–a medicine capable of curing any disease according to ancient manuscripts from Vedic times. The present study was conducted to validate the medicinal properties of cow urine in a scientific manner. The cow urine sample was procured from Indian indigenous cow Bos indicus from Bombay Panjrapole, Bhuleshwar, Mumbai, and commercial gaumutra from the local market. Various treatments were given to the freshly collected cow urine viz. Photoactivated urine (PA), Cow urine distillate (DIS) and Crude cow urine sample (CRU) was used after filtration and centrifugation. In-vitro assays for antimicrobial (agar cup method), antioxidant (DPPH assay), anti-hyperglycemic (α-amylase inhibition) and anti-inflammatory (protein denaturation inhibition bioassay) properties of cow urine were carried out. Results confirmed antimicrobial (19 mm zone of clearance), antioxidant (157 µg /ml), anti-hyperglycemic (2.08 µg /ml) and anti inflammatory (23.72µg /ml) and comparable to Chloramphenicol, Ascorbic acid, Acarbose, and Ibuprofen used as standard respectively. The study demonstrates various medicinal properties of cow urine using in-vitro...
Read More
