Posted by admin on Oct 31, 2021 in |
The plants Portulaca oleracea and Gomphrena globosa belong to the order Caryophyllales, and their flowers are known to produce betalains. The biosynthesis of betalains involves various intermediate molecules such as catecholamines or biogenic amines. Catecholamines include dopamine, adrena-line (epinephrine), and noradrenaline (norepinephrine) and are normally believed to be released in response to stress. They are derived from the amino acid tyrosine, with L-DOPA being formed as an intermediate. Catecholamines and their predecessors (tyrosine and L-DOPA) have many medicinal applications and are used in the treatment of Parkinson’s disease, hypotension, low cardiac output, glaucoma, and allergic conditions like asthma, to name a few. These biogenic amines and their predecessors have been found in many plant families. Their presence has been reported in Portulaca oleracea and Gomphrena globosa. The current study focuses at the identification and quantification, by LCMS/MS, of the different catecholamines or their predecessors that are present in Portulaca oleracea and Gomphrena...
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Posted by admin on Oct 31, 2021 in |
Natural products from various medicinal plants, in the form of pure compounds or standardized extracts, which provide unlimited opportunities for discovery of new drug leads, is due to the unmatched availability of chemical diversity. There is an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest, particularly in edible plants, has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation, and characterization of active ingredients of bioactive compounds from Stachytarpheta urticifolia. The common problems and key challenges in the extraction, isolation, and characterization of active ingredients of bioactive compounds are discussed. As extraction is the most important step in the analysis of constituents present in herbal preparations, the strengths and weaknesses of different extraction techniques are discussed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC...
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Posted by admin on Oct 31, 2021 in |
L-glutaminase [EC.3.5.1.2] is an amidohydrolase that catalyzes the hydrolytic deamination of L-glutamine, resulting in the production of L-glutamic acid and ammonia. The L-glutaminase has received a significant attention due to its potential as an anticancer agent. In the present study, soil sediments were collected from the estuaries region of Parangipettai, Cuddallore District, Tamil Nadu. The isolated fungal strains were screened for L-glutaminase production, and the potent strain was characterized and identified as Aspergillus flavus JK-79 (Genebank accession number MF445235). The anti-cancer enzyme L-glutaminase production by Aspergillus flavus JK-79 was evaluated in five different production media under the submerged fermentation process. The Minimal medium showed maximal enzyme production by the marine isolate. Further, parameters such as Inoculum size, pH, temperature, and nutritional requirements such as carbon sources, nitrogen sources, and amino acids were optimized by One-factor-at-a-time (OFAT) for maximal production of L-glutaminase under submerged fermentation process. The maximum production of L-glutaminase by Aspergillus flavus JK-79 was recorded at pH 8 (790 .436U/mL), temperature 28°C with 2% inoculum size. The findings...
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Posted by admin on Oct 31, 2021 in |
Plants are a source of bioactive compounds produced by different parts of the plant, maybe bark, leaves, flowers, roots, fruits, and seeds, during metabolic activities. These secondary metabolites may have protective and disease preventive properties, hence used by Traditional practioner to treat various ailments. Most plants of the Western Ghats of India remain unknown, poorly understood or unestablished for their effects on health because of unexplored phytochemical studies or insufficient scientific data. Improvement in health is possible through the intake of phytochemical supplements, which reduce oxidative stress. In recent years research on phytochemicals and search for novel high quality, inexpensive source of medicine is increasing as these do not have harmful side effects. In the present study Nothopegia racemosa, a member of Anacardiaceae, is investigated for the presence of phytochemical compounds, which would lead to further pharmacological studies. The investigation was carried out using dried and powdered bark extract using water, hydro alcohol, chloroform, petroleum ether, and hexane as solvents. The qualitative analysis revealed the presence of tannins, alkaloids,...
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Posted by admin on Oct 31, 2021 in |
The present study aimed to evaluate the effect of crude leaf extract of Hellenia speciosa on hematological parameters of alloxan-induced diabetic rats. The study was carried out using alloxan model of diabetes for a period of 28 days and at the end of the treatment rats were sacrificed and blood samples were collected for the estimation of hematological parameters. The results showed that the white blood cell (WBC) count was significantly (p<0.05) increased while red blood cell (RBC) and hemoglobin (Hb) were significantly (p<0.05) decreased in diabetic rats compared to normal rats. Treatment with the crude leaf extract of Hellenia speciosa ameliorated the imbalances in hematological parameters caused by alloxan. Moreover, Induction of diabetes does not show any significant change in the differential count, MCV, MCH, MCHC, MPV, HCT and Platelets does not show alterations in normal and diabetic control. Therefore, the crude leaf extract of Hellenia speciosa can be considered relevant in the management of the...
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