Posted by admin on Nov 30, 2024 in |
An accurate, sensitive, precise, fast isocratic reverse phase HPLC technique has been developed and validated for the quantification of Vibegron in the pure and pharmaceutical dosage form. The best separation was achieved on a 250 mm x 4.6 mm ID., 5µ-particle size Inertsil®-Octadecyl-Silyl-3V-Reverse-Phase-C18-column with 0.05M Ammonium dihydrogen orthophosphate in water: acetonitrile (60:40 v/ v) as mobile phase solvent at a speed of 1.0 ml/ min in the isocratic mode of elution. UV detection was observed at 238 nm. Retention time of Vibegron was found to be 4.0 minutes. With a correlation coefficient of about 0.9989, peak-response was obtained as function of concentration of Vibegron over the range of 80 to 240 µg/ ml. Vibegron had shown to have a percentage assay of 110.05 %. It had a limit of detection (LOD) and a limit of quantification (LOQ) of 0.1 µg/ ml and 0.3 µg/ ml, respectively. The presence of excipients in the formulation had no effect on the assay method. The procedure is swift and precise and can be employed appropriately for...
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Posted by admin on Nov 30, 2024 in |
Nanoparticles are primary to nanoscience and nanotechnology, presenting powerful process over different fields. However conventionally synthesized by using physical and chemical methods, these strategies always suffer from harmful issues. To highlight these approach, eco-friendly and less toxic green synthesis methods have been established. These methods involve natural sources such as medicinal plant Tradescantia zebrina, the use of these medicinal plantsknown for the specially assuring path due to its significant to overcome toxicity and microbial resistance problems found in current therapeutic practices. Eco-friendly green synthesis of silver nanoparticles (AgNPs) from silver nitrate (AgNO3) using Tradescantia zebrina leaves extract as reducing agent by a simple method at room temperature. The biosynthesized nanoparticles (NPs) were characterized by scanning electron microscopy (SEM) coupled with (EDAX) and Fourier transform infrared spectroscopy (FTIR). The AgNPs synthesized were spherical, block shaped, and irregularin shapes. The XRD spectrum confirmed the presence of silver ions and crystalline nature of synthesized AgNPs. FTIR showed the functional groups such as C-O, N–H and C–N groups involved in the reduction of...
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Posted by admin on Nov 30, 2024 in |
Introduction: The primary cause of type 2 diabetes is the body’s inability to respond to insulin or produce adequate insulin. Diabetes mellitus (DM) is a metabolic disease that causes persistent hyperglycemia. It has been demonstrated that Trigonella foenum-graecum (TFG), often known as fenugreek, has anti-hyperglycemic effects on both humans and animals with type I and type II diabetes. Fenugreek improves peripheral glucose tolerance and utilization in diabetics who are not insulin-dependent. Method: Vacuum-dried TFG ethanolic extract was stored at -20°C until required. Streptozotocin (STZ, 70 mg/kg) dissolved in citrate buffer (pH 4.5) was injected intraperitoneally once into the abdomen of adult rats weighing 150–200 g in order to induce severe diabetes. The control rats received citrate buffer alone in proportion to their body weight. Oral (po) administration of TFG dry seed ethanolic extracts was started on the third day and continued for 21 days. Results: On day 21, (TFG) demonstrated a significant reduction in blood glucose levels, a significant difference in oxidative stress by raising SOD and GSH and...
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Posted by admin on Nov 30, 2024 in |
Introduction: Topical haemocoagulase (Botroclot) is a fractionated snake venom extract which acts as procoagulant and augments healing. In our study we ascertain the various causes of bleeding in routine clinical practice and evaluate the efficacy of this agent in arresting minor bleeding. Aim and Objective: To ascertain the various causes of bleeding in routine ear, nose, throat (E.N.T.) practice in a tertiary care center and evaluate the efficacy of topical haemocoagulase in arresting minor E.N.T. and head and neck bleed. Materials and Methods: This study was conducted from October 2019 to April 2024 in the Department of Otorhinolaryngology. A total of 582 patients presenting with nasal, aural, oral cavity, oropharyngeal, laryngeal and head and neck bleed were included in this study. The cause of bleeding pertaining to individual subsites were identified and charted in tabulated format. Haemocoagulase solution was used for topical instillation over the bleeding site. Time taken from the application of solution to the cessation of bleeding was noted. Results: Amongst all the cases, Nasal bleed was...
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Posted by admin on Nov 30, 2024 in |
Diltiazem HCl is a newer class of antiarrhythmic agent and available in market tablets as a controlled and extended-release formulation. The release pattern is influenced by atmosphere, pH, the presence of food and other physiological conditions of GI tract. This is possible by formulating and osmotic tablets. A controlled porosity osmotic tablet of diltiazem HCl was formulated by incorporating leachable polymer Eudragit in a PEG-400 semipermeable coating membrane. The core tablet was prepared using the drug, mannitol, MCC, PVP K-30, talc and mg. stearate. The core tablet was evaluated using bulk and tapped density, carr’s index, angle of repose, hardness, weight variation, in-vitro dissolution. The thickness of PEG-400 coating was optimized to give a desired release of drug. The drug release study was done by USP-II dissolution apparatus (paddle). 23 factorial design used to optimized the formulation. The release pattern was concluded from kinetics models as a zero-order release. Osmotic tablet of diltiazem can be successfully formulated using diltiazem HCl (120mg), HPMCK100M (100mg), PEG 400(20%), mannitol (3.5mg) giving 6%...
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