Posted by admin on Apr 30, 2026 in |
A rapid and robust RP-HPLC method was developed and validated for the simultaneous quantification of Perindopril and Indapamide. Chromatographic separation was achieved on a Zodiac C18 column (4.6 × 150 mm, 5 µm) at 38°C using a mobile phase of acetonitrile and water (40:60 v/v) with a flow rate of 1.0 mL/min. Detection was performed at 223 nm, and the injection volume was 10 µL, with a total run time of 10 minutes. The method was validated for accuracy, precision, linearity, and reproducibility. Calibration curves demonstrated excellent linearity with correlation coefficients of 0.9991 for Perindopril and 0.9998 for Indapamide. Accuracy and precision studies showed % purity values of 99.86% for both drugs, confirming the reliability of the method. The developed RP-HPLC method is rapid, precise, and suitable for routine simultaneous estimation of Perindopril and Indapamide in pharmaceutical...
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Posted by admin on Apr 30, 2026 in |
This study evaluated the stability of siamenoside I, a minor sweet component of Siraitia grosvenorii (Monk Fruit), under pH and temperature conditions representative of commercial beverage storage, as well as under photostability conditions. Mock beverage solutions, formulated to ~500 mg/L siamenoside I, were prepared at four pH values (2.8-4.2)) and stored at temperatures of 5°C-40°C for 26 weeks and degradation products were quantified at scheduled intervals. Under all conditions, siamenoside I exhibited high stability. Six known degradation products/impurities (compounds 3,6,7,8,9 and 14) were detected at levels ≥0.500 mg/L (≥0.100% of the starting concentration). Photostability testing indicated minimal degradation under fluorescent light exposure. No changes in appearance were observed; all samples remained colorless and clear. Siamenoside I in mock beverages demonstrated chemical stability under conditions simulating both typical and extreme commercial beverage storage and handling, including light exposure, supporting their suitability for use in acidic, ready-to-drink...
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Posted by admin on Apr 30, 2026 in |
Background: The rise in antibiotic resistance has rendered many antibiotics less effective, making it increasingly challenging to treat infections. Objectives: The objectives of this study were to determine the association between ceftriaxone resistance and in-hospital mortality and length of hospital stay among critically ill patients with bacterial infections and to analyze ceftriaxone resistance patterns among patients with bacterial infections. Methods: A written informed consent was taken from participants who fulfilled inclusion criteria and were willing to participate. Demographic profile and clinical characteristics of participants were documented. Results: The duration of hospital stay in ceftriaxone sensitive group and ceftriaxone resistant groups was represented as mean ± SD (in days), in ceftriaxone sensitive group it was 9.73 ± 3.66 days and in ceftriaxone resistant group it was 16.00 ± 11.06 days. The patients in the ceftriaxone resistant group had a longer hospital stay. On comparing the clinical outcome between the two groups the survival rate was better in ceftriaxone sensitive group. Conclusion: The clinical outcome and duration of hospital stay between...
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Posted by admin on Apr 30, 2026 in |
Infectious diseases caused by bacteria remain a major public health concern, and searching for alternative antimicrobial agents from medicinal plants is essential. Aloe vera is a widely used medicinal plant known for its therapeutic properties, including skin care and antimicrobial activity. This study evaluated the antibacterial activity of Aloe vera stem crude extracts against Staphylococcus aureus (ATCC-25923) and Streptococcus pyogenes (ATCC-19615). Crude extracts were prepared by maceration using chloroform, acetone, and ethanol as solvents. Extract yield was determined, and antibacterial activity was assessed using the disc diffusion method at concentrations of 100, 200, and 300 mg/ml. Ciprofloxacin and tetracycline were used as positive controls, while dimethyl sulfoxide (DMSO) served as a negative control. Minimum inhibitory concentrations (MICs) were determined using the serial dilution method. Among the solvents, ethanol produced the highest extract yield of 0.8%. All extracts demonstrated antibacterial activity against the tested pathogens, with the chloroform extract showing the strongest inhibitory effect. Stem chloroform and stem acetone extrac have better minimum inhibitory concentrations (50 mg/ml). These findings suggest...
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Posted by admin on Apr 30, 2026 in |
Background: Diabetes mellitus (DM) is one of the most prevalent chronic metabolic disorders worldwide. Optimal glycaemic control often requires combination therapy using oral hypoglycemic agents with complementary mechanisms of action. Objectives: To compare the efficacy and safety of Glimepiride Metformin and Teneligliptin Metformin combinations in patients with T2DMinadequately controlled on monotherapy. Materials and Methods: This prospective, open-label, parallel, randomized single-centre study included 204 patients with uncontrolled type 2 DM, randomized equally into two groups. Group A received Glimepiride 2 mg once daily plus Metformin 1 g twice daily, while Group B received Teneligliptin 20 mg once daily plus Metformin 1 g twice daily. Fasting blood sugar (FBS) and post-prandial blood sugar (PPBS) were measured at baseline, 3 and 6 months, and glycated haemoglobin (HbA1c) at baseline and 6 months. Adverse events were recorded. Data were analyzed using chi-square and unpaired t-tests. Results: Baseline parameters were comparable between groups. At 6 months, differences in mean FBS and mean PPBSin Group A and Group B respectively were not statistically significant. Mean...
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