Posted by admin on Apr 30, 2026 in |
Drosophila melanogaster serves as an ideal model for studying olfactory perception due to shared cellular and molecular odor-coding strategies with mammals. Olfactory dysfunction commonly appears as a non-motor symptom in neurological disorders like Epilepsy and Parkinson’s disease, often before motor symptoms develop. This study examined Lantana camara’s flower and leaf extracts on olfactory perception in Epileptic para bangsenseless1 Drosophila mutant. L. camara flowers and leaves were shade dried, grounded, and prepared as aqueous extracts using Soxhlet apparatus. 2-5 day old adult flies were maintained on cornmeal-yeast-agar medium containing extracts at different concentrations for 24 hours, followed by 24-hour starvation. Olfactory behavioral assays were conducted using a vial-based setup with attractant (yeast paste) and repellent (citronella oil) stimuli, measuring response times to olfactory perception. High-dose flower extracts yielded the most significant enhancement, achieving response times similar to wild-type control flies. L. camara extracts modulate olfactory sensory neuron properties, potentially normalizing signal transduction disruptions in the epileptic model. These dose-dependent improvements suggest the plant can restore sensory circuit functionality in neurological...
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Posted by admin on Apr 30, 2026 in |
Background: Probiotics defined as live microorganism with beneficial effects on their host’s health. In the present investigation commercial probiotic sachet was used as isolation source. The aim of this study was to evaluate the potential of probiotic bacteria and to assess the probiotic properties of the isolates. Methods: Probiotic bacteria were isolated from commercial sachet to assess their biochemical characteristics and probiotic properties like acid, bile, phenol, NaCl tolerance, antimicrobial activity and antibiotic susceptibility pattern with further identification at species level (16S rDNA sequencing). Results: The findings revealed that both the isolates showed moderate to high growth at pH 2, pH 3.5 and pH 5. Bile tolerance was highest at 0.05% bile concentration followed by 0.15% and 0.3% after 3 hrs of incubation. The isolates were able to survive at 0.4% phenol concentration. Moreover, they survived in saline concentrations of (1-4 % NaCl), showed minimal growth in 5% and 8% NaCl and exhibited no growth at 6%, 7% and 9 % NaCl concentrations. Both isolates demonstrated inhibitory activity against...
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Posted by admin on Apr 30, 2026 in |
Background: Social media has emerged as a dominant source of drug- and supplement-related information, with pharmaceutical and wellness influencers often disseminating unverified or promotional content. Medical students, as early trainees, may be particularly susceptible to such misinformation. Objective: To assess the extent of exposure to pharma influencers and evaluate associated knowledge, attitudes, and practices among MBBS students. Methods: A cross-sectional questionnaire-based study was conducted among 155 MBBS students. The validated survey assessed influencer exposure, drug-safety knowledge, attitudes toward online medical content, and self-reported behaviours. Knowledge differences were analyzed using independent t-tests. Results: Of the participants, 78.1% followed pharma influencers and 89% regularly encountered drug-related posts. Influencer-followers demonstrated significantly lower drug-safety knowledge (mean 3.5 vs 4.6; p = 0.0003). Nearly one-third reported purchasing influencer-promoted products, with adverse effects reported by 22.4% of users. Conclusion: High exposure to pharma influencers is associated with reduced pharmacological literacy and unsafe self-medication practices, underscoring the need for structured digital health literacy training in medical...
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Posted by admin on Apr 30, 2026 in |
A stability indicating reverse phase high-performance liquid chromatography (RP‑HPLC) method was developed and validated for the determination of thirteen related impurities in Nintedanib esylate, applicable to both bulk drug and finished product. Chromatographic separation was achieved on a Waters X‑Bridge C18 column (250 mm × 4.6 mm i.d., 5 µm) under gradient elution. The mobile phase consisted of 0.01 M ammonium bicarbonate buffer (pH 8.0 ± 0.05, adjusted with sodium hydroxide) as phase A, and a mixture of methanol, acetonitrile, and buffer (45:45:10, v/v) as phase B. Detection was performed at 240 nm, ensuring baseline resolution of Nintedanib from its impurities, with peak resolution consistently greater than 2. Forced degradation studies under acidic, alkaline, aqueous hydrolysis, and oxidative conditions confirmed the method’s ability to separate Nintedanib from its degradation products, including the major related substance (RS9). The method was validated in accordance with ICH guidelines for selectivity, linearity, accuracy, precision, and solution stability. This validated RP‑HPLC method provides a robust and reliable approach for routine analysis of related impurities...
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Posted by admin on Apr 30, 2026 in |
Background: Pharmaceuticals released into the environment pose ecological and public health concerns. Ecopharmacology, which addresses the adverse effects of pharmaceuticals on the environment, remains underexplored among healthcare students. This study aimed to assess the knowledge, attitude, and practices regarding ecopharmacology and safe drug disposal among nursing students. Materials and Methods: A cross-sectional study was conducted among 172 nursing students in Mandya city. The responses were collected using a pre-tested structured questionnaire that comprised demographics, knowledge (7 items), attitude (8 items), and practice (7 items). Data were analysed using descriptive statistics. Results: Among the 172 nursing students who participated in the study, awareness of ecopharmacology and existing guidelines for drug disposal was limited. Although attitudes towards environmental protection were largely positive, unsafe disposal practices were commonly reported. Conclusion: The present study showed that nursing students had a positive attitude towards ecopharmacology, but demonstrated limited knowledge and engaged in unsafe drug disposal practices. Enhancing awareness programmes and establishing accessible drug disposal facilities are essential to promote safe drug disposal...
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