Posted by admin on Aug 31, 2021 in |
Two-dimensional (2D) culture systems are indispensably used as in-vitro tools to improve our perception and understanding of cell biology, tissue engineering, tissue morphology, mechanisms of diseases, and drug action. However, the conventional 2D culture systems suffer from limitations such as alterations in cell morphology and lack of interactions between extracellular and cellular environments. As a result, three-dimensional (3D) cell cultures have received remarkable attention in studies such as drug discovery and development; the sandwich model being one of the unique cellular models widely used for analyzing tissue metabolism and functions. In this study, we devised a novel Gel Casting Tool (GCT) for in-vitro toxicity analysis. Liver cells need complex 3D micro-architecture to express tissue-specific functions and were suitable for this study. Human Carcinoma Cell line (HepG2 cells) with Gelatin methacrylamide (GelMA) hydrogel was used to check the efficacy of the GCT. The results showed that GCT Sandwiched HepG2 exhibited high cell viability, proliferation, and tissue-specific functions such as albumin and urea over 7 days. The major advantage of this...
Read More
Posted by admin on Aug 31, 2021 in |
Reverse Phase High-Performance Liquid chromatography is a widely and commonly used analytical method for qualitative and quantitative analysis. A simple, accurate, economical, rapid, and precise RP-HPLC process has been proposed and validated for the determination of esomeprazole in immense bulk and dispensed pharmaceutical drug form. The RP-HPLC separation was achieved on Symmetry ODS RP C18, 5 µm, 15 mm × 4.6 mm i.d. column using mobile phase Phosphate buffer (pH=3.6): ACN = 50:50 pH Adjudged with Orthophosphoric acid at continuance effusion of 1.0 ml/min at ambient temperature. The retention times were observed at 3.797 min for esomeprazole. The calibration plot is linear beyond the concentration range 0-50 μg/ml for esomeprazole. Quantification was achieved with UV detection at 246 nm over the Beer-Lambert’s range. The proposed methodology is validated statistically as per the ICH guidelines for various validation parameters includes, including Accuracy, Precision, Linearity and range, Limit of detection, and Limit of Quantification. The above method is developed and validated successfully for the routine quantitative analysis of esomeprazole in bulk...
Read More
Posted by admin on Aug 31, 2021 in |
The aim of the present study was to characterize the petroleum ether, chloroform, and ethanol extracts of rhizomes of Curcuma leucorrhiza Roxb and to evaluate their biological activities such as anti-oxidant, anti-bacterial, and anti-fungal activities. Sequential solvent extraction was performed by Soxhlet apparatus using petroleum ether, chloroform, and ethanol, and respective extracts were named as CL-P, CL-C and CL-E. Antioxidant activities of the extracts were evaluated by DPPH, ABTS, and lipid per oxidation assays. Antibacterial and antifungal activities were evaluated against Gram-negative bacteria Escherichia coli (MTCC 739), Gram-positive bacteria Bacillus subtilis (MTCC 121), Micrococcus luteus (MTCC 106), Mycobacterium smegmatis (MTCC 6), fungal plant pathogen Rhizoctonia solani (MTCC 4633) and fungal animal pathogen Aspergillus niger (MTCC 1344) using agar well diffusion method with some modifications. Phytochemical analysis showed that solvent extracts of Curcuma leucorrhiza rhizome contained polyphenol, phenols, quinones, flavonoids, terpenoids, carbohydrates, glycosides, cardiac glycosides, coumarins and alkaloids. All the three extracts used in the study displayed the ability to scavenge DPPH and ABTS radicals and to inhibit lipid peroxidation....
Read More
Posted by admin on Aug 31, 2021 in |
Background: Psoriasis is an immune-mediated inflammatory disease characterized by excessive growth and abnormal differentiation of keratinocytes. Objective: In the present research work, an attempt has been made to formulate and evaluate gel containing cyclosporine, aloe vera and tree tea oil. Methods: The gel was prepared by a conventional method and evaluated for various parameters viz., physical appearance, pH, drug content, viscosity, spreadability and in-vitro diffusion studies, In-vitro skin retention studies and stability studies. The prepared optimized gel was further compared with marketed formulation; all the formulations were good in appearance and had good homogeneity. Results: The optimized gel is shown spreadability co-efficient is 5.8 g cm/sec. The gel has shown 95 ± 0.32% releases in 6 h when evaluated for in-vitro diffusion studies. The biological evaluation of the optimized gel has shown 47.98 % drug activity, indicating good efficacy of the formulation against psoriasis. Conclusion: In conclusion, the prepared optimized gel has shown good spreading co-efficient and stability and can be effective for psoriasis as compared to available gel...
Read More
Posted by admin on Aug 31, 2021 in |
The crude drug powder extracts from three plants Datura metel L., (Solanaceae), Leucas cephalotes (Roth.). Spreng, Piper betel L., (Piperaceae) were screened for estimating phytochemical constituents. Phytochemical analysis of the extracts revealed that glycoside; tannin, saponin, carbohydrate and phenolic compounds are present in Piper and Leucas species. Besides glycoside, tannin, saponin, carbohydrate and phenolic compound, alkaloid is also present in Datura species. The antimicrobial activity of the plant materials and their usefulness in the treatment of asthma may be due to the presence of active constituents like tannins, alkaloids and phenolic. The present study reported that the phytochemical constituents of three plants being widely used for traditional treatment of asthma in Myanmar, collected from Kalay, Sagaing...
Read More
