Posted by admin on Aug 31, 2021 in |
Five marine sponges were collected off Carmen, Agusan del Norte, Philippines and taxonomically identified as Axinyssa sp., Plocamionida sp., Forcepia sp., Pachymatisma sp and Placospongia sp. The “non-polar” (EtOAc-MeOH) and “pola”r (EtOH-H2O) extracts of the marine sponges were prepared and subjected to 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, a total antioxidant capacity assay using the phosphor molybdenum method and antimicrobial assay using the paper disc diffusion method. The marine sponge extracts exhibited low antioxidant activity as indicated by both the percent antiradical activity values obtained which ranged from 0.89% to 5.41% and the total antioxidant capacity values expressed as Ascorbic Acid Equivalence (AAE) ranging from 11.99 to 126.67 AAE and Butylated Hydroxytoluene Equivalence (BHTE) with a value range of 6.64 to 148.30 BHTE). The non-polar extracts from all the marine sponges as well as the polar extract from the marine sponge Forcepia sp. showed antibacterial activity against the two bacterial strains, B. subtilis and E. coli. However, the marine sponge extracts no significant antifungal activity against the two fungal...
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Posted by admin on Aug 31, 2021 in |
Senna alata is an effective novel pharmaceutical medicinal plant. The hot extraction method was used to prepare methanol and ethanol extracts of leaves. The extracts were further evaluated to study Antimicrobial potential, phytochemical analysis, FTIR, and Antioxidant activity. The FTIR analysis showed various functional groups that served as secondary metabolites and responsible for antimicrobial activity. The antimicrobial activity of the extract may be due to the presence of secondary metabolites like alkaloids, terpenoids, phenols, saponins, flavonoids, glycosides and tannins. The Senna alata leaves were evaluated for antimicrobial activity against clinically important bacterial strains such as E. coli, B. cereus, B. subtilis, S. aureus, P. aeruginosa, P. fluorescens, and fungi strain namely A. niger by agar well diffusion method. The ethanolic extract of the leaves of Senna alata showed the highest antioxidant activity. S. alata has a great antimicrobial ability which needs to be intensively discussed...
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Posted by admin on Aug 31, 2021 in |
Moringa oleifera and chywanprash are two main nutraceuticals used worldwide because of their large medicinal application. The quality of these nutraceutical products is in question, especially due to the inorganic contaminants like heavy metals. In the present study, six Moringa herbal powder and five chywanprash samples were collected in India and further analyzed for As, Cd, Hg, and Pb. All samples were microwave digested and analyzed by ICP-MS. In Moringa As, Cd, Hg and Pb were found in the range of 0.027-5.264 µg/mL, 0.014-0.039 µg/mL, 0.049-45.225 µg/mL and 0.323-12.487 µg/mL respectively. In chywanprash As, Cd, Hg and Pb were found in the range of 0.012-0.021 µg/mL, 0.004-0.029 µg/mL, 0.01-0.103 µg/mL and 0.06-0.171 µg/mL respectively. The concentration of these heavy metals were found to be more than the maximum permissible limit given by the WHO for herbal medicines. Hence strong regulatory guideline needs to be prepared to improve quality of...
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Posted by admin on Aug 31, 2021 in |
Aim: The present study was undertaken to screen the phytochemicals present in fruits of Grewia tiliaefolia and to evaluate the potential role of these fruits in the amelioration of cisplatin-induced nephrotoxicity in male Albino rats. Materials and methods: Ethanol extract of Fruits of Grewia tiliaefolia was prepared by hot extraction method. The prepared ethanol extract was subjected to preliminary phytochemical evaluation followed by TLC, HPTLC, HPLC and GC-MS analysis. The nephroprotective potential of the extract was screened at 200 and 400mg/kg b. w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intraperitoneal injection of cisplatin at a dose of 5 mg/kg b.w. The nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels and his to pathological studies in renal tissue. Results: Upon preliminary phytochemical screening, the ethanol extract showed the presence of Proteins, flavonoids, Tannins, Terpenoids. HPTLC analysis resulted in a fingerprint of extract showing the presence of 14 compounds. TLC and HPLC revealed the presence...
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Posted by admin on Aug 31, 2021 in |
Background: Dolutegravir and Rilpivirine are two antiretroviral drugs that have been approved for the treatment of HIV Infection. An error-free, accurate, precise and valid reverse-phase liquid chromatography method was developed for the quantitation of Dolutegravir and Rilpivirine in its bulk form as well as in combined dosage form by forced degradation studies. Methods: Chromatographic separation of these two drugs Dolutegravir and Rilpivirine, was achieved with an INERTSIL ODS C18 (250 × 4.6 mm, 5 μm) reverse-phase analytical column with a 10 min analytical run time using a mixture of 0.1% OPA: Acetonitrile in the ratio of (60:40 v/v) as mobile phase. The mobile phase was streamed at a flow rate of 1.0 mL min-1 with a column temperature of 250 °C and detection wavelength was carried out at 230 nm. The retention time was found to be 3.4 min for Dolutegravir and 4.3 min for Rilpivirine. Results: The linearity limit of Dolutegravir and Rilpivirine was found to be in the range of 0.999 and 0.999. The method validation was...
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