Posted by admin on Aug 31, 2021 in |
Mesenchymal stem cells are a small number of undifferentiated cells located in various tissues of the body. They have the ability to self-renew and differentiate into lineages of mesenchymal origin. They serve as most promising candidates for various regenerative medicine and tissue engineering applications. Their use in various clinical applications depend on their proliferative capacity to expand cell population. One of the key challenge to expansion is decline in proliferation and differentiation capacity of cells isolated from older individuals. In the present study, we have compared cellular characteristics of bone marrow-derived mesenchymal stem cells isolated from young (4 – 6 weeks age) and old (60-64 weeks age) rats. The two rat groups were compared for proliferation kinetics, differentiation potential and senescence markers SA- βgal, reactive oxygen species (ROS), as well as expression ofP16INK4A, P21 and telomerase genes. A significant decrease in frequency, population doubling time, and cell yield associated with increase in age were observed. A decline in differentiation potential for osteogenic and chondrogenic lineages, and an enhancement in...
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Posted by admin on Aug 31, 2021 in |
Cyclea peltata (Lam), belonging to the family Menispermaceae, is a common plant in South India. It is mentioned in Ayurvedic classics by Rajapatha and is one of the important drugs used in Ayurveda therapeutics and is used widely in different formulations in medical practice. The quality control of crude herbal drugs and their bio constituents is paramount in justifying their acceptability. The crude drugs can be identified systematically on the basis of their morphological, histological, chemical, physical and biological studies. Phytochemical screening reported the presence of alkaloids, carbohydrates, glycosides, phyto sterols compounds and proteins and amino acids. The plant is widely used in the treatment of cough, fever, kidney disorder, urinary disorder and snake poisoning. The aim of the present study is to extract and isolation of bioactive compound (Thymol) from Cyclea peltate (Lam) Hook f. Thoms, the isolation and characterization analysis, included Thin Layer Chromatography, High-Performance Thin Layer Chromatography, GC-MS and Spectroscopy studies (IR, NMR and MASS). The presence of thymol in the ethanolic fraction revealed that the...
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Posted by admin on Aug 31, 2021 in |
Plants present in the mangrove ecosystem are underexplored for their natural bioactive agents, including a neglected scope of inventing newer antimicrobials, to combat global crisis mediated by MDR microorganisms. In this study, we investigated four plants-Excoecaria agallocha, Bruguiera gymnorhiza, Avicennia alba and Aegialitis rotundifolia of the Sundarbans, world’s largest mangrove ecosystem in West Bengal, India, for their antimicrobial activities against Salmonella typhi and Candida parapsilosis, in addition to their important bioactive resources including antioxidants. Ethanolic, methanolic, and DMSO extracts of leaves of these plants were studied by antimicrobial screening, determination of total phenolic and flavonoid contents, DPPH free radical scavenging activity, ABTS [2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] assay, ferric reducing antioxidant power (FRAP), lipid peroxidation inhibition, thin layer chromatography, and liquid chromatography-mass spectroscopy (LC-MS). E. agallocha extracts showed excellent antimicrobial activities against S. typhi, while antifungal activity against C. parapsilosis was almost lacking. MIC values of all the extracts against S. typhi was as low as 3.96 µg/mL, however, growth inhibition was most with E. agallocha extract. Again total phenolic content...
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Posted by admin on Aug 31, 2021 in |
Triazole is a diunsaturated heterocyclic compound that contains two nitrogen groups as heteroatoms. In the present study, an attempt was made to synthesize various derivatives of substituted 1, 2, 4-triazol-3-yl) benzene-1-ol and assess their antimicrobial efficiency. The synthesized compounds were subjected to physical characterization and spectral analysis by IR and NMR for structure elucidation. The compounds were then subjected to evaluation of antimicrobial activity against bacterial strains Escherichia coli (Gram -ve), Pseudomonas aeruginosa (Gram -ve), Bacillus Subtili (Gram +ve), Staphylococcus aureus (Gram +ve) method using ciprofloxacin as standard and against fungal strain Aspergillus niger method using fluconazole as standard. The results of antibacterial activity show that compounds P1 and P2 and the results of antifungal activity show that compounds P3 and P4 showed equivalent activity when compared with standard while the rest of the compounds were found to be less active than...
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Posted by admin on Aug 31, 2021 in |
A fixed-dose combination of montelukast sodium, levocetirizine dihydrochloride, and acebrophyl line is used in the treatment of asthma, COPD, allergic rhinitis and other conditions where patients feel difficulty in breathing. The present study was designed to develop and validate a simple, sensitive, precise and accurate isocratic reverse-phase high-performance liquid chromatography (HPLC) method for simultaneous estimation of montelukast sodium (MTKT), levocetrizine dihydrochloride (LTZ) and acebrophyl line (ABP) in fixed-dose combination tablets. Chromatographic separation of these drugs was achieved on Hypersil ODS C18 column (250 × 4.6 mm, 5µm) as stationary phase. A mixture of methanol, acetonitrile and 20 mM ammonium acetate buffer in the ratio of 60:30: 10v/v was used as the mobile phase at a flow rate of 0.8 ml/min with detection at 232 nm at a column temperature set at 35 ℃. The total elution time for the three drugs was about 10 min. The developed method was validated by conducting system suitability, selectivity, linearity, precision, accuracy, limits of detection, and limits of quantification as per ICH guidelines....
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