Posted by admin on Aug 31, 2021 in |
Plants are rich sources of chemical compounds of high therapeutic value and screening plants for such bioactive compounds is the order of the day. First and foremost, it appears sensible to probe into the chemical reserves of abundantly available but rarely studied plants. The present study was carried out to determine the presence of phytochemicals in a common roadside weed, Cleome burmanni using Gas Chromatography- Mass Spectrometry (GC-MS) analysis. The mass spectrum of the crude methanol extract was compared with the available library sources (NIST08 LIB, WILEY8 LIB). GC-MS analysis of the methanol extract of C. burmanni revealed the presence of 24 compounds. The compounds along with their retention time, molecular weight, molecular formula and peak area have been recorded. Cholest-4-en-3-one (19.05%) was the major compound detected followed by Urs-12-en-28-al (17.98%), Lup-20(29)-en-3-one (14.87%), 1-(1-Butynyl) cyclopentanol (13.47%) and 4, 22-stigmastadiene-3-one (10.77%). Remaining 19 compounds were detected in low quantities. Almost all the constituents were reported to possess specific biological...
Read More
Posted by admin on Aug 31, 2021 in |
Context: Drug-drug interactions (DDIs) are frequently observed in hospitalized cardiac patients and associated with alteration in the effectiveness of cardiovascular therapy. Aim: The study was conducted to identify the incidence and nature of DDIs and their associated risk factors among patients with cardiovascular disease (CVD). Materials and Method: A prospective interventional study was conducted between August 2018 and April 2019 in the Department of Cardiology of a tertiary care South Indian hospital. Patients admitted with CVD on a minimum of two medications were enrolled in the study and their prescriptions were evaluated for DDIs using Micromedex interaction checker version 2.8. Results: A total of 258 patients with CVD were enrolled in the study with a mean age of 60.11±12.07 years. On average, each patient was prescribed 11.35±3.30 medications. The incidence of potential DDIs (pDDIs) was 99.61%, with 7.59±4.10 as a mean number of pDDIs per prescription. The majority of the interactions were significant in severity (50.69%), pharmacodynamic (68.24%) in nature, of unspecified onset (69.97%), and with fair documentation status...
Read More
Posted by admin on Aug 31, 2021 in |
Bio behavioural aspects, immunity, and disease course are interlinked in a majority of conditions, particularly cancer. Peripheral stressors or their products regulate immunity by various ways. When there is added insult in an already compromised immune state such as cancer, as is done here using lipopolysaccharide (LPS), the system is said to be ‘double-hit. Behavioural and brain tissue-associated biochemical changes on LPS challenge have been assayed in a Diethylnitrosamine (DEN)-treated Wistar rat model of hepatic cancer. Peripheral and central effects of LPS challenge have been screened at levels of neuro behaviours, liver enzymes and brain enzymes. While LPS challenge reduced exploratory and grooming behaviours across groups after 2 h, the effect disappeared on testing after 4 h, 6 h post LPS injection, rats demonstrated increased open arm time, reduced closed arm time and increased entries into both arms of the elevated plus-maze. DEN treatment induced similar results. LPS challenge in DEN-treated rats however, led to reduced open arm time, increased closed-arm time, and reduced entries into both arms. LPS...
Read More
Posted by admin on Aug 31, 2021 in |
A RP-UFLC method was developed &validated for simultaneous estimation of Sitagliptin Phosphate and Metformin HCl in bulk and tablet dosage form. Separation was achieved by using Phenomenex Luna C18 (150 × 4.6mm, 5µm) column. The mobile phase comprises MeOH: Phosphate Buffer of pH 7 using Triethylamine in the ratio (60:40v/v). The flow rate was 0.6ml/min at temperature 300C. The instrument used for UFLC was Shimadzu autosampler separation model LC 20AD. Quantification was achieved with UV detection using a photodiode array detector at 258nm wavelength. The injection volume was 20µl. The retention times of Sitagliptin Phosphate & Metformin HCl were found to be 7.027 & 2.802, respectively. The linearity range of Sitagliptin Phosphate was 50-100µg/ml & Metformin HCl was 10-60µg/ml. The correlation coefficient was found to be within limits, i.e., 0.999. The % purity of Sitagliptin Phosphate & Metformin HCl was found to be 101.99% and 101.73%. LOD values were found to be 2.21437 for Sitagliptin Phosphate & 1.99084 for Metformin HCl and LOQ values were found to be 6.71024...
Read More
Posted by admin on Aug 31, 2021 in |
Marine sponges have become a promising source of secondary metabolites with diverse structures and have been shown to have a wide range of biological activities. In search of bioactive compounds, a preliminary cytotoxic assessment of marine sponge extracts was conducted. The cytotoxic activities of the hexane, dichloromethane (DCM), ethyl acetate, and aqueous extracts of the marine sponges, Stelodoryx procera Topsent 1904 and Rhabdastrella sp., collected off the coast of Zamboanga City, Philippines were tested using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay. The hexane extract, ZP12NPh, from S. procera and DCM extract, ZP07NPd, from Rhabdastrella sp. exhibited moderate toxicity against colorectal cancer (HCT116) cell line at 30 µg/mL. Further purification of the hexane extract from S. procera has afforded two (2) sub-fractions, ZP12NPh4 and ZP12NPh5, which have shown to be highly cytotoxic against HCT116 at 30 mg/mL. While the DCM extract from Rhabdastrella sp., has afforded fourteen (14) highly cytotoxic sub-fractions (ZP07NPd2, ZP07NPd3, ZP07NPd4, ZP07NPd5, ZP07NPd9, ZP07NPd10, ZP07NPd13, ZP07NPd15, ZP07NPd16, ZP07NPd17, ZP07NPd18, ZP07NPd19, ZP07NPd20 and ZP07NPd21) against HCT116 at...
Read More
