Posted by admin on May 31, 2021 in |
The main objective of this study was to Assessment of the acute toxicity of ethanolic extract of Polyherbal formulation in Swiss albino mice. In this study, the acute oral toxicity study of ethanolic extract of PHF1 was carried out according to Organization for Economic Co-operation and Development (OECD) guideline 423 and 407 on Swiss albino mice (20–30 g). In the study, the oral dose of 2000 mg/kg of PHF1 extract was administered in the treated group. General behavior, morbidity, and mortality were determined up to 72 h and compared to control group. Further animals were investigated for a period of 3days assess any other toxic effect. Behavioral changes and other parameters such as body weight, hematological parameters, and biochemical parameters were evaluated and compared to the normal group. The result of the study was mentioned that In acute toxicity study, treatment groups revealed neither morbidity nor mortality or any significant changes in behavior only drowsiness, sedation, and lethargy were observe in the group having 2000 mg/kg of Polyherbal formulations...
Read More
Posted by admin on May 31, 2021 in |
Congenital Hypothyroidism may be defined as a heterogeneous group of disorders characterized by goiter or cretinism due to an absolute or relative deficit of the enzyme Thyroid Peroxidase (TPO), and it is action. Chronic hypothyroidism also leads to goiter and cretinism associated with end gland damage, dysfunction, cancer, and failure, including the retina, kidney, nervous system, heart, and vascular tissue damage. The screening for Congenital Hypothyroidism (CH) may either be in the form of a thyroid function test or via thyroid peroxidase antibodies test, as recently recommended by the Thyroid Federation Association (TFA). Unto now, the treatment of goiter and cretinism is levothyroxine supplement and gland surgery removal. This review will establish the effectiveness of the enzymatic drug (TPO) and its indication for the treatment of PCH when compared to other therapies. This review also highlights the enzyme thyroid peroxidase (TPO) role and current treatment strategies on Congenital Hypothyroidism...
Read More
Posted by admin on May 31, 2021 in |
A simple, sensitive, and fast single-step extraction method for estimating empagliflozin in human plasma using LC-MS/MS was developed and validated for pharmacokinetics evaluations. Plasma samples were basified before solid-phase extraction on SOLA (30 mg / 1 mL cartridges). Separations were carried out on a normal reverse phase C18 column (Hypersil BDS 100 × 4.6, 5µ mm column) for 3.5 minutes at a flow rate of 0.6 mL/min. Ten µL of the SPE eluent is directly injected onto LC-MS/MS to quantify the analyte from 1.563-800.000 ng/mL using a single SRM transition (m/z: 449.140à371.100) in negative ion mode. During method validation, selectivity, matrix effect, recovery, carry-over effect, stability studies, inter-day, and intra-day precision and accuracy experiments were conducted per USFDA guidelines. Method validation data has successfully met the acceptance criteria making it suitable for use in routine bio-analytical laboratories. The scope of this assay can be extended to cover the requirement of preclinical, toxicology, and PK/PD...
Read More
Posted by admin on May 31, 2021 in |
Cadaba indica Lam (Indian Cadaba), an Indian traditional medicinal plant has been used for various diseases. The cold macerated methanolic leaf extract of Cadaba indica was assessed using Agilent GC 7890A gas chromatography connected with an MS- 5975C mass spectrometer and the mass spectra were matched with NIST 14.0 – data library. Several chemical constituents were identified within 28 min of the entire GC-MS analysis. The GC chromatogram shows that presence of most abundant linolenic acids and its esters such as Hexa-decanoic acid, methyl ester, n-hexadecanoic acid, 10,13-octadecadienoic acid, methyl ester, 9, 12, 15-Octadecatrienoic acid, (Z, Z)-methyl ester, Octadecanoic acid, methyl ester, 9, 12-Octadecadienoic acid, (Z, Z)-, 9, 12, 15-Octadectatrionic acid, (Z, Z, Z)-,Octadecanoic acid, Hexadecanoic acid, 2,3-dihydroxy propyl ester and 9- Octadecanoic acid, (Z) – methyl ester. In contrast, compounds with diterpenes, phthalic esters, pyrrolidine, phenol, ketones, and myristic acid derivatives were also detected with average peak area percentage. The nature and chemical background of the detected phytochemical constituents may be responsible for the pharmacological effects of Cadaba...
Read More
Posted by admin on May 31, 2021 in |
Aim: The present study was to develop and evaluate antiulcer polyherbal tablet as designing of oral herbal formulation. The methanolic extracts of Abrus precatorius (MEAP) and Cordia wallichi (MECW) leaves were used in different concentrations in the formulations. Materials and Method: The current exploration was to design a gastro-retentive drug delivery system of MEAP and MECW using swelling polymer through the wet granulation method. All the formulations were evaluated for weight variation, hardness, friability, drug content, and in-vitro dissolution. In this gastro-retentive dosage form using hydroxypropyl methylcellulose-K4M was prepared to develop sustain release tablets, which could remain in the stomach for longer periods of time, delivering the drug to the site of action. Results: Pre and Post-compression parameters of all the formulations were within the Pharmacopoeial limits, and in-vitro drug release of F8 formulation was found to be 98.29% in 12 h. Conclusion: Dissolution studies of the composition, it was concluded that the formulation F8, which is containing 200 mg of polyherbal extracts, 140 mg of HPMC-K4M, 85 mg...
Read More
