Posted by admin on Oct 31, 2021 in |
A simple, sensitive and rapid chromatographic method was developed and validated for quantification of Mitomycin and Fluorouracil in bulk and pharmaceutical dosage form using Symmetry C18 (4.6 × 150 mm, 3.5 µ) column. The mobile phase consists of buffer, 0.1% Ortho Phosphoric acid (OPA), and acetonitrile in the ratio of 50:50 v/v. The flow rate is maintained as at 1.0 ml/min; detection was carried out by absorption at 260 nm using photodiode array detector. The calibration curve was linear and the regression coefficient (R2) value was found to be 0.999 and concentrations ranging from 10-150 µg/ml of mitomycin and 5-75 µg/ml of fluorouracil, respectively. The LOD and LOQ of the method were found to be 0.1 µg/ml, 0.05 µg/ml and 1 µg/ml, 0.5 µg/ml for Mitomycin and Fluorouracil. The number of theoretical plates and tailing factors for Mitomycin and Fluorouracil were not less than 2000 and were not more than 2, respectively. The developed method was found to be simple, economical, suitable and validated according to ICH...
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Posted by admin on Oct 31, 2021 in |
Citrus fruit is a very rich source of Vitamin C, A, E, and flavonoids, alkaloids, and minerals. Citrus fruits are medicinal plants that present rutaceae family and include some fruits such as orange (Citrus sinensis), kinnow (Citrus reticulata), mosambi (Citrua ltmetta), grapefruit (Citrus maxima) lime, pomelo lemon. Antibacterial antiviral, antifungal activity of different solvent extracts (Acetone, ethanol, methanol, and distilled water) prepared by soxhlet extractor and aqueous solvent from three citrus fruit peel orange, kinnow, mosambi (Citrus sinensis, Citrus reticulata, and Citrus limetta respectively) were screened against four pathogenic bacteria staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi. The highest antibacterial activity was exhibited by the acetone peel and ethanol extract of Citrus reticulate peels. MICs were tested at concentrations ranging from 80-5.25 mg/mL as wells as their MBCs. The phytochemical analysis of the citrus peel extracts showed the presence of flavonoids, saponins, steroids, terpenoids, tannins and...
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Posted by admin on Oct 31, 2021 in |
Celiac disease an antibody-mediated enteropathy which is a lifelong condition where there is intolerance towards gluten. The only treatment till now is the gluten-free diet. The aim of this study was to investigate proximate, phytochemical and functional property analysis of gluten-free composite flour. The proximate analysis of composite flour for the most acceptable sample showed moisture (10.97±0.0), ash (3.35±0.2), fat (4.03±0.1), fiber (4.88±0.2) and protein (18.01±0.5), carbohydrate (61.72±0.4), respectively. Micronutrients like calcium (248.6±1.9), iron (5.03±0.9) and phosphorus (359.6±0.2), respectively, were also present in composite flour. Gluten content had been found 0.02 g/100g. This is the negligible amount that is allowable for celiac patients. The composite flour also contains several phytochemicals such as flavonoids, saponin, tannin, glycosides, alkaloids, and steroids. Some functional properties were assessed, namely water absorption capacity (WAC), oil absorption capacity (OAC), emulsion capacity (EC), foaming capacity (FC) and gelation capacity (GC). So this composite flour is useful for celiac patients and being nutritionally sufficient, conditions like anemia, diabetes and osteoporosis can also be prevented. Composite flour can...
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Posted by admin on Oct 31, 2021 in |
Objective: To elaborate and characterize new co-processed excipients for direct compression use. Methods: The new co-processed excipients were obtained with different proportions of microcrystalline cellulose (Avicel® PH101) and Isomalt (galenIQ® 800), using Starch® 1500 and Plasdone® K-25as binding agents in a fluid bed. Rheological characterization was performed; to determine their deformation pressure (Py), the Heckel model was applied. Tablets were elaborated with each polymer and each co-processed compound at different compaction pressures, and the behavior of the tensile strength curves was analyzed. Results: The range of particle size of the obtained co-processed compounds was 95.5 to 151µm. The flow velocity of the co-processed excipient elaborated with Avicel® PH101/galenIQ® 800 improved as compared to the velocity presented by the individual raw matters. Conclusions: The Avicel® PH101/galenIQ® 800 co-processed excipients elaborated at 60/40% and 70/30% proportions can be recommended to be used as direct compressible excipients because these proportions presented the best characteristics regarding flowability, less deformation pressure (Py) and a higher slope in the tensile strength...
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Posted by admin on Oct 31, 2021 in |
The plants Portulaca oleracea and Gomphrena globosa belong to the order Caryophyllales, and their flowers are known to produce betalains. The biosynthesis of betalains involves various intermediate molecules such as catecholamines or biogenic amines. Catecholamines include dopamine, adrena-line (epinephrine), and noradrenaline (norepinephrine) and are normally believed to be released in response to stress. They are derived from the amino acid tyrosine, with L-DOPA being formed as an intermediate. Catecholamines and their predecessors (tyrosine and L-DOPA) have many medicinal applications and are used in the treatment of Parkinson’s disease, hypotension, low cardiac output, glaucoma, and allergic conditions like asthma, to name a few. These biogenic amines and their predecessors have been found in many plant families. Their presence has been reported in Portulaca oleracea and Gomphrena globosa. The current study focuses at the identification and quantification, by LCMS/MS, of the different catecholamines or their predecessors that are present in Portulaca oleracea and Gomphrena...
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