Posted by admin on May 31, 2021 in |
India is a great source of naturally occurring plant drugs which are having potential pharmacological activities. Origanum vulgare L. (Lamiaceae) is one of the most important medicinal plants in India, consisting of aromatic and medicinal properties which is used to cure many ailments. In spite of its plethora of uses as medicine and food additives since ancient times, it has reaped very little scientific interest so far, particularly in India. In such a propitious context, the intention of this study was to enhance the plant as a medicinal herb by determining pharmacognostic standards and evaluating the phytochemical components. Hence, the present study aimed at the pharmacognostical standardization of Origanum vulgare L., including the morphology, anatomy, and powder studies pertaining to organoleptic, microscopic, and physical constant evaluations. This study also describes the phyto-chemical screening and HPTLC analysis of major phytochemicals present in the taxa. The pharmacognostic profile obtained from this work can serve as a tool for developing standards for the identification, quality, and purity of Origanum vulgare L. The...
Read More
Posted by admin on May 31, 2021 in |
The objective of this study was to determine the hepatoprotective effect of strawberry extract (150 mg/kg.b.w) in rats against Indomethacin sodium-induced gastric ulceration. Oral indomethacin administration (5 mg/kg.b.w.) resulted in a substantial rise in total plasma cholesterol, triglycerides (HDL), factor-alfa (TNF-α), nitric oxide (NO), liver and stomach necrosis, tumor necrosis factor-alfa (TNF-α), nitric oxide (NO), thiobarbaturic acid reactive substances (TBARS), 8-hydroxy 2 deoxyguanosine (8-OHdG) and NF kappaB. Also, treatment of rats with Indomethacin led to a significant decrease in liver and stomach high mobility group box 1 (HMGB1) gene expression as well as a decrease in the levels of GSH, superoxide dismutase (SOD), catalase (CAT). The obtained results supported by histopathological data obtained by light and electron microscope which revealed that strawberry extract (150 mg/kg.b.w.) prevent liver tissue damage and stomach ulceration through increasing of GSH, SOD and CAT activities and decrease significantly TBARs, TNF-α, NO, 8-OHdG, NF kappaB levels, and HMGB1 gene expression. Conclusion: These results suggest that strawberry extract contains bioactive phenolics, minerals, and vitamins, which may...
Read More
Posted by admin on May 31, 2021 in |
A simple, accurate & precise method was developed for the simultaneous estimation of Darunavir (DAR), Cobicistat (COB), Emtricitabine (EMT), and Tenofovir alafenamide (TEN) in their bulk and pharmaceutical formulation. The chromatogram was run through Agilent C18 (150 × 4.6 mm, 5 m) column. Mobile phase containing 0.1% Ortho Phosphoric acid (OPA) (pH 2.4) and Acetonitrile in the ratio of 55:45 was pumped through the column at a flow rate of 0.75 ml/min. While the temperature was maintained at 30°C. The optimized wavelength for the combination was 245 nm. The retention time of DAR, COB, EMT, and TEN were found to be 3.988 min, 3.147 min, 2.205 min, and 2.616 min respectively. The linearity range for the method was found to be 200-1200µg/ml for DAR, 37.5-225 µg/ml for COB, 50-300 µg/ml for EMT and 2.5-15 µg/ml for TEN with regression coefficients of 0.9992, 0.9997, 0.9997 and 0.9994. The method was validated according to ICH guidelines and the drugs were subjected to forced degradation as per ICH Q1A15. Forced Degradation studies...
Read More
Posted by admin on May 31, 2021 in |
The potential of transferosomal formulation for transdermal delivery of Ginger constituents was investigated. The aim was to reduce the dosage and improve patient acceptance. Transferosomes were formulated by ethanol injection technique using Phospholipids (Lipoid P-100), cholesterol, various edge activators (tween 80, Span 80), and other excipients. Transdermal patches were prepared by solvent casting method using polymers (Ethylcellulose, HPMC), penetration enhancers (Oleic acid, Tween 80) and PEG 400 as a plasticizer. Transferosomes were evaluated for in-vitro release, particle size, zeta potential, entrapment efficiency, and stability. Patches were evaluated for folding endurance, uniformity of weight, drug content, and thickness. Transferosomes with 1:2:0.25 ratio of Phospholipid: ginger extract: Tween 80 showed particles of 318.8nm, the zeta potential of -6.3mV and 74.4% entrapment efficiency and 67% in-vitro release. Optimized batch of patches showed folding endurance 7, 0.134 ±0.0012 mm thickness, 97.18 ± 0.78% drug content. Thus, transferosomes with transdermal delivery is a better approach to deliver ginger...
Read More
Posted by admin on May 31, 2021 in |
Aframomum melegueta (Alligator pepper) is a tropical plant that belongs to the Zingiberaceae (Ginger) family, consumed for its culinary and health benefits. This study investigated the therapeutic efficacy of ethanolic seed extract of Aframomum melegueta at ameliorating paraquat-induced ovarian toxicity in rat models. Thirty female Wistar rats were divided into control and five test groups (n=5). Group 1 (Control) received feed and water, while other groups (2-6) were treated orally and daily for 4 weeks as follows; group 2 (400 mg/kg b.w. of Aframomum meleguate seed extract); group 3 (200 mg/kg b.w. of Aframomum meleguate seed extract); group 4 (20 mg/kg b.w. of paraquat); group 5 (co-administration of 20 mg/kg b.w. of paraquat and 400 mg/kg b.w. of Aframomum meleguate seed extract); group 6 (co-administration of 20 mg/kg b.w. of paraquat and 200 mg/kg b.w. of Aframomum meleguate seed extract). At the end of the experiment, oxidative stress markers (malondi-aldehyde (MDA) and superoxide dismutase (SOD)), estradiol levels, and histology of the ovaries were assessed. Data obtained were analyzed using...
Read More
