Posted by admin on May 31, 2021 in |
Capparis decidua Edgew. (Capparidaceae) is an important medicinal plant widely used in Ayurveda as anti-inflammatory, anti-arthritic, hepatoprotective, diuretic, digestive, laxative, analgesic and anti-asthmatic. In this study, a sensitive and reliable high-performance thin-layer chromatography (HPTLC) method has been developed for the quantification of a flavonol quercetin as an important marker in C. decidua stem. Ethyl acetate extract prepared from hydrolyzed alcoholic extract was applied on precoated silica gel G 60F254 plate and the plate was developed using chloroform-methanol-formic acid (9:1:0.6, v/v/v) as the mobile phase. The detection and quantification was carried out by densitometric scanning at 366 nm. The system was found to provide good resolution of quercetin from other constituents present in C. decidua stem. The method was validated for linearity, inter-day and intra-day precision, repeatability, accuracy, specificity, limit of detection and limit of quantification. The linear regression analysis data for the calibration plots showed a good linear relationship (r2 = 0.995) in the concentration range of 500-1500 ng. The accuracy of the method was confirmed from recovery studies...
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Posted by admin on May 31, 2021 in |
A simple, accurate, and precise reversed-phase high-pressure liquid chromatography (RP-HPLC) method was developed for the simultaneous estimation of nimesulide and dicyclomine hydro-chloride in fixed-dose combination tablets. The developed method was validated as per ICH guidelines Q2(R1). The chromatographic separation was achieved on Hypersil ODS C18 (200 × 4.6 mm 5 μ) column using methanol: phosphate buffer (pH 4) 40:60 (v/v) as the mobile phase at a flow rate of 1 mL/min at 25 °C and detection at 215 nm. The retention times of nimesulide and dicyclominehydrochloride were 3.32 min and 4.22 min, respectively. The linearity range of nimesulide was 2.5-80 μg/mL, and that of dicyclominehydrochloride was 150- 650 μg/mL. The percent recovery of nimesulide and dicyclomine hydrochloride was found to be in the range of 98-102%. The developed method was validated as per ICH guidelines Q2 (R1) and can be used for the estimation of nimesulide and dicyclomine hydrochloride in fixed-dose combination tablets. The developed method can be extended to other dosage forms containing nimesulide and dicyclomine hydrochloride with...
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Posted by admin on May 31, 2021 in |
Medicinal plants, herbs, spices, and herbal remedies are integral components of the alternative system of medicine since while’s ancient Cassia auriculata Linn is a potential folklore medicinal plant (Caesalpiniaceae) used for Ayurveda and Siddha systems of medicine. Plants contain a wide variety of phytochemical constituents, which are secondary metabolites and are used either directly or indirectly in the pharmaceutical industry. For centuries, man has effectively used various components of plants or their extracts for the treatment of many diseases. The aim of the study was to investigate the phytochemical compounds of Cassia auriculata and GC-MS analysis. The presence of phytochemical compounds was screened by a qualitative method using gas chromatography and mass spectroscopy (GC-MS). An aqueous and ethyl acetate extract obtained were subjected to GC-MS for the determination of bioactive volatile compounds. GC-MS analysis was carried out using claries 680 GC with clarus 600 (EI) MS. The GC-MS analysis of the aqueous and ethyl acetate extract revealed the presence of 26 bioactive compounds with valuable biological activities. The major...
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Posted by admin on May 31, 2021 in |
Nanotechnology can be applied to deliver and protect antioxidants in order to control the oxidative stress phenomena in several chronic pathologies. Chitosan (CS) and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, and biodegradable, and, hence, suitable for various biomedical applications, such as drug delivery gene therapy and tissue engineering. Vitis vinifera (VV) seeds are the major chemotherapeutic agent with antioxidant, anti-inflammatory, antiproliferative, anticancer, and antimicrobial effects. However, the potential of these leaves as a chemotherapeutic agent is limited by their hydrophobicity and poor bioavailability. The chitosan tripolyphosphate (CS/TPP) nanoparticles have been used in this study as an alternative to encapsulate bioactive flavonoids from ethanolic extract of Vitis vinifera seeds. The Vitis vinifera seeds extract-loaded CS (CS-VVE) nanoparticles were characterized using FT-IR. The Zeta sizer and zeta potential confirmed that the particle has 216nm and 50.3mV. This study also found that the CS-VVE NPs shown anticancer activity in human colon cancer cell lines should be explored further for Cytotoxicity studies using MTT assay and tested drug-induced apoptotic...
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Posted by admin on May 31, 2021 in |
The objective of the research work was to design NLCs gel of Acitretin and perform an in-vitro evaluation of different formulations. FTIR and DSC studies revealed that there was no interaction between drug and polymer and with the respective surfactant. A new generation of NLCs consisting of a lipid matrix with a special nanostructure has been developed. These nanostructures improve drug loading and firmly incorporates the drug during storage. The purpose of this study was to prepare Acitretin-loaded NLCs gel effective for topical delivery of Acitretin by using the Hot Homogenization method. Stearic acid as solid lipid, Oleic acid as liquid lipid combination of two types of surfactant: Tween 80 and Sodium lauryl sulphate (SLS) were used to stabilize NLCs dispersion. Physiochemical properties of NLCs gel such as particle size, TEM, entrapment efficiency, and in-vitro release studies were investigated. The formulation in which a combination of ionic and non-ionic surfactant was used to demonstrated good entrapment efficiency, sustained in-vitro release profile. In-vitro release study revealed the biphasic release pattern...
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