Posted by admin on Apr 30, 2021 in |
Antimycotic activity of Lead oxide (PbO) nanoparticles prepared by the bio-safe method was evaluated for rot causing fungi. It was observed from the study that all the concentrations of nanoparticles brought about significant inhibition in the spore germination and mycelial growth of all the rot causing fungi. However, the highest inhibition in the germination of all the test fungi was observed at higher concentrations, followed by lower concentrations of nanoparticles. The highest inhibition in the spore germination was found against Aspergillus niger and it varies from 74.26% to 37.63% in different concentrations of nano Pb, whereas the least reduction of spore germination was found against P. expansum and it ranges from 52.44% to 20.47% in different concentrations of nanoparticles respectively, as compared to untreated control. Similarly, inhibition in spore germination of all other tested fungi also varies significantly with the increase in the concentrations of Pb nanoparticles, and the highest concentration caused the highest reduction in the spore germination followed by lower concentrations of Pb nanoparticles, respectively. However, the...
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Posted by admin on Apr 30, 2021 in |
Synthesis of silver nanoparticles using fruit extract has been gaining considerable attention as an eco-friendly approach in green chemistry. The present work reports the synthesis of Muntingia calabura silver nanoparticles (McAgNPs) by the addition of an appropriate amount of Muntingia calabura fruit extract to the aqueous AgNO3 solution under constant stirring at room temperature for 24 h. The addition of fruit extract leads to the formation of stable colloidal silver nanoparticles. The peak obtained at 460 nm using UV-VIS spectroscopy confirmed the formation of silver nanoparticles. Scanning electron microscopy analysis showed the spherical and oval morphology of the silver nanoparticles. Energy-dispersive X-ray spectroscopy (EDS) analysis indicated the abundance of silver nanoparticles in chemical composition. DLS/ZETA POTENTIAL analysis was applied in order to measure the particle size distribution and nature of the surface charge of the McAgNPs. Further, the antibacterial activity against the silver nanoparticles was...
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Posted by admin on Apr 30, 2021 in |
An alternative, economically viable, and laboratory-friendly synthetic method described for the preparation of Bimatoprost, an anti-glaucoma drug. Stereospecific synthesis of Bimatoprost has been achieved with commercially available (-) Corey lactone i.e., diol, as a starting material. To our delight, the replacement of the benzoyl (Bz) group with Tetrahydropyranyl ether (Thp) protection resulted in yield improvement. Tetrahydropyranyl ether (Thp) is notorious as a useful protecting group for alcohols in organic synthesis. It has numerous benefits, such as low cost, ease of insertion, general stability to most non-acidic conditions. It offers good solubility and easy to remove if the functional group it protects requires manipulation. Moreover, it described the successful application of luche’s reduction for the conversion of α, β-unsaturated ketone 8 to allylic alcohol 9 using CeCl3 7H2O, NaBH4. This lanthanide combination with borohydride enables the selective 1, 2-hydride attack on the carbonyl group, instead of an undesired 1, 4-hydride attack, which leads to the formation of desired allylic alcohol without disturbing the double...
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Posted by admin on Apr 30, 2021 in |
The aim of the present study is to apply the Design of Experiments (DoE) to develop an assay method for the separation of amino acids by RP-HPLC. The method used in this study is the DoE Combined- Randomized method. Design of Experiments allows interpreting the results with better outcomes and enhanced understanding process. Ion pair reagent- Octane 1 Sulphonic acid sodium salt monohydrate of 0.01M at pH 2.2 is used along with Methanol and Tetra-hydro Furan. The column used is INETSIL C8, with 5µ Particle size and 4.6×250 mm column dimensions. The Solvent composition and ion pair concentration is evaluated as variables in the DoE. The wavelength for all amino acids is 205 nm. Conclusively DoE is an efficient tool for the separation of amino acids. Method development was established, and the design is validated. The proposed method has adequate reproducibility and accuracy for the estimation of the amino acids in the routine...
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Posted by admin on Apr 30, 2021 in |
Angiogenesis is an inevitable process of tumour growth, invasion, and metastasis. Understanding the process of new blood vessel formation has gained prime importance recently, and anti-angiogenic therapy has now been considered as an important strategy for cancer therapy. Many bioactive compounds, especially of plant origin, have been explored for their anti-angiogenic potential. The present study was formulated to evaluate the effect of Majorana hortensis leaf extract on angiogenesis, in an effort to examine its influence against cancer cell proliferation, invasion, and metastasis. Cell viability analysis in non-transformed chick embryo fibro-blasts showed that Majorana hortensis leaf extract alone exhibited slight cytotoxicity as assessed by MTT and SRB assay. However, in combination with etoposide, the leaf extract exhibited a synergistic effect as evident from the improved cell viability in chick embryo fibroblasts. Using CAM assay, the anti-angiogenic potential of the Majorana hortensis leaf extract alone was inferred from the significant reduction in blood vessel formation. The anti-invasive potential of M. hortensis leaf extract was further substantiated by a scratch assay using...
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