Posted by admin on Jul 31, 2021 in |
Nephrolithiasis or kidney stone disease is a disease characterized by the formation of crystals in the kidney. It is a disease that is found in many countries with high rates of recurrence and treatment costs. This study analyzes the effect of Breadfruit leaf extract on oxalate levels of CaOx stones deposited in the kidney and leukocyte differentiation in hyper oxaluric rats induced ethylene glycol. Experimental research with a -post-test with control design using 25 rats, which randomly divides into five groups, namely Normal, Ethylene Glycol, extract doses of 100, 200, and 400 mg/kg BW groups. The rat’s induced ethylene glycol 0.75% v/v for 28 days to initiate hyperoxaluria and the formation of CaOx stones. The treatment groups were given extracts from days 15 to 28. The data show tht the induction of ethylene glycol increases the levels of kidney oxalate (CaOx). Giving Breadfruit leaf extract 400 mg/kg BW can significantly reduce kidney oxalate (CaOx) levels and influence the total level of leukocytes. Leukocyte differentiation. The Breadfruit leaf extract prevents...
Read More
Posted by admin on Jul 31, 2021 in |
The comparative study of in-vitro seed germination was conducted to determine the influence of different concentrations of chemically synthesized copper nanoparticles (CuNPs) on the in-vitro seed germination and growth of two different varieties of wheat- PBW677 and PBW725 on MS medium. For all growth parameters taken under the present study, PBW 725 variety of wheat performed better in the basal MS media having different concentration of CuNPs than PBW 677. 100% germination percentage of PBW 725 variety of wheat is achieved on Basal MS medium supplemented with 5 ppm CuNPs. This variety has better seed vigour and tolerance index. It indicates that a lower concentration of CuNPs is potential enhancer of seed germination of this particular variety. But the higher concentration of the nanoparticles proved to be detrimental and resulted into poor seed germination and decreased morphological development of the plants of both...
Read More
Posted by admin on Jul 31, 2021 in |
Objectives: The objective of the study is to find out the alpha-amylase inhibitory activity of Katankateryadi Kwatha. The alpha-amylase enzyme is the key source for postprandial hyperglycaemia, which is responsible for Type 2 Diabetes. Methodology: The crude drugs of Katankateryadi Kwatha (K. Kwatha) were collected from a local market. The Kwatha was prepared and dried with rotatory evaporator, under reduced pressure. Different concentrations (10, 20, 40, 60, 80 and 100 μg/ml.) of Kwatha were prepared and used for α-amylase inhibitory assay using starch as a substrate. Similarly, the protocol performed with Acarbose as standard. The absorbance was read at 595 nm using spectrophotometer. The percentage of α-amylase inhibitory activity and IC50 values of each concentration was calculated. Results & Discussion: The study shows that the Katankateryadi Kwatha has alpha-amylase enzyme inhibitory property. Standard Acarbose shows IC50 value of 38.46±0.956 with % inhibition ranged from 23% to 80% at concentration 10, 20, 40, 60, 80 and 100 µg/ml. while the inhibitory concentration (IC50 value) of K. Kwatha was found to...
Read More
Posted by admin on Jul 31, 2021 in |
High-Performance Liquid Chromatography is an important and powerful analytical technique for the identification, quantification and isolation of natural products. In the present investigation, a Reversed-Phase- HPLC method was developed for the identification and quantification of the marker compound quercetin in Euphorbia thymifolia Linn. (whole plant) and Manilkara hexandra Roxb. (leaves). Both the plants have been used to cure various ailments since a long time in India. The results confirmed the presence of quercetin in both the plants, and its concentration was found higher in M. hexandra than E. thymifolia extract. Thus, the study justified the traditional use of these plants as medicines, and both the plants could be used in the formulation of some quality drugs in the future; however, further studies are required in this...
Read More
Posted by admin on Jul 31, 2021 in |
Integration of nano-science in medicine leads to the development of biomedical products that help society in a faster and safer manner. In the present studies, Zinc nanoparticles were synthesized by green synthesis route using aqueous extracts of Banium persicum, and characterization was done through UV, SEM, TEM, FT-IR, and XRD. The synthesized nanoparticle was spherical in shape with an average size of 20 nm. Various bioactive compounds present in aqueous extract of this plant were responsible for bioreduction of nanoparticles. Further, these nanoparticles were tested for various biological activities. In antimicrobial activity maximum, anti-bacterial activity was observed against S. aureus at 120 µg/ml (IZ-18 mm),while in the case of fungus, maximum potential was observed at against C. albigans at 120 µg/ml (IZ- 30 mm). Further nanoparticles were screened for their antioxidant activity by LPO, Peroxidase, and Catalase assay and found they possessed potent antioxidant activity. The platelet aggregation of nanoparticles was assayed by Prothrombin (PT) and Activated Partial Thromboplastin time (APTT). In PT assay, maximum activity was observed at...
Read More
