Posted by admin on Mar 31, 2021 in |
A simple, accurate, and economical, least time-consuming method has been developed for Losartan potassium (LP) by using UV spectrophotometer. This method was developed using phosphate buffer solution pH 7.4 (PBS) for quantifying the amount of LP released from the colon-specific formulations. The wavelength maximum (λmax) of LP in phosphate buffer pH 7.4 was found to be 228.5 nm. The developed UV spectroscopic method exhibited linearity in the range of 2-10 μg/mL with a correlation coefficient (R2) value of 0.999 and was validated with respect to linearity, specificity, accuracy (recovery), and precision. These parameters were determined according to International Conference on Harmonization (ICH) guidelines. Results of the analysis were validated statistically and by recovery studies, which proved the suitability of the developed method for the routine estimation of LP in colon-specific control release formulation. Hence, the reported method for the estimation of LP from the colon-specific formulations was simple, accurate, and least...
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Posted by admin on Mar 31, 2021 in |
Poor bioavailability by the oral route is noticeable with the majority of new active pharmaceutical ingredients due to its dissolution rate-limited absorption. Gliclazide is an oral anti-hyperglycemic drug, though it is a class II drug, results in poor oral bioavailability. The present investigation was undertaken to prepare polymeric nanosponges of an oral anti-hyperglycemic drug Gliclazide to achieve improved solubility. Nanosponges using ethyl cellulose as a polymer and glutarldehyde as a cross-linker were prepared successfully by the emulsion solvent diffusion method. Drug polymer compatibility study was performed by FTIR and DSC. To obtain optimized batch, 32 factorial designs were performed. Optimized batch exhibited particle size 398. 78 nm, % drug content 90.504 ± 0.296, % entrapment efficiency 81.25 ± 0.266, % drug release 97.725 ± 0.186. An SEM and TEM image of optimized batch shows the spongy and spherical nature of formulations were converted into tablets to achieve immediate release drug delivery for oral route. These tablets were prepared using crospovidone and A SEM and TEM image of the optimized...
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Posted by admin on Mar 31, 2021 in |
The present study shows the cytotoxic effect of Dendrophthoe falcata (L. f) and Tridax procumbens Linn. extracts against HaCat (Skin cancer) cell line by MTT and SRB assay method. The phytochemical screening of extracts dichloromethane: methanol (DM), methanol: water (MW), and ethanol: water (EW) revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, sterols, and triterpenes. % Cell inhibition by MTT assay of DFDM extract shows 90.00 ± 0.09% and TPDM 91.29 ± 0.02% at 80 µg/ml. IC50 values of DFDM extract <20 µg/ml and TPDM extract 20 µg/ml compare to IC50 values of standard 5-fluorouracil <10 µg/ml by MTT assay. SRB assay method DFDM extracts shows 86.96 ± 0.07% and TPDM shows 89.14 ± 0.27 with IC50 values < 20 µg/ml compare standard 5-fluorouracil <10µg/ml. HPTLC fingerprint analysis of active DFDM extract showed 11 peaks at 366 nm Rf values 0.06-0.87 & TPDM shows showed 12 peaks Rf values 0.33- 0.83 at 366 nm. Active extracts were analyzed agilent 7890A GC coupled with triple quadrupole...
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Posted by admin on Mar 31, 2021 in |
Herbal medicines are in substantial demand in the developed country for primary healthcare because of their high efficacy, safety, and few side effects. Sesbania grandiflora is an ancient plant that belongs to the family Fabaceae. Traditionally, it is used for the treatment of various disorders such as diabetes, anxiety, hepatotoxicity, ulcer, and anti-inflammatory activity. Therefore the present investigation was undertaken to investigate the anti-arthritic potential of Sesbania grandiflora seeds extract against CFA-induced arthritis in experimental animals. The Ethanol extract of Sesbania grandiflora seeds was prepared by liquid-liquid extraction procedure, and phytochemical analysis of extract was done by the different chemical test as well as HPLC. Daily oral treatment of ethanol extract of Sesbania grandiflora and UK-356618 for twenty one days. After nine day of CFA administration improve the body weight and attenuated the paw volume, hepatic biomarkers as well as hematological parameters such as hemoglobin, RBC, Hb, WBC, and ESR of experimental animals. Finding of the current investigation demonstrated that the ethanol extract of Sesbania grandiflora along with UK-356618...
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Posted by admin on Mar 31, 2021 in |
In the view of an extensive literature survey that revealed the anticonvulsant profile of 1,4-dihydropyridines and 1,3,4-Thiadiazole, we have coupled these two moieties with the aim of achieving an enhanced anticonvulsant effect. In present investigation some of ethyl-1-((5-amino-1, 3, 4-thiadiazol-2-yl) methyl)-5-ethyl-2, 6-dimethyl-4-substituted phenyl-1, 4-dihydro-pyridine-3-carboxylate derivatives are synthesized through four steps. A greener approach is employed by using Chloramine-T as an efficient and safer oxidative catalyst for the synthesis of Thiadiazole and the use of the Microwave technique for accelerating the chemical reactions. The structures of the newly synthesized compounds were confirmed on the basis of IR, 1H-NMR, and Mass analyses. An acute toxicity study was done to determine LD50 of the newly synthesized compounds. Some of the synthesized compounds were evaluated for their anticonvulsant effect by PTZ induced convulsions method. Statistical testing was done by one-way ANOVA followed by Dunnett’s test. The compounds F123 and showed the highest percentage of protection against convulsions at the dose of 15 mg/kg among the evaluated compounds compared to...
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