Posted by admin on May 31, 2021 in |
In the present study, we evaluated the Anti-parkinson’s activity of Cyamopsis tetragonoloba methanol plant pod extract by Haloperidol Induced Catalepsy and Tacrine induced vacuous jaw movements for behavioral parameters and in-vivo assays to estimate neurochemical parameters like (GSH, LPO, and SOD). The behavioral analysis performed using Haloperidol Induced Catalepsy produces Parkinson’s disease (PD) like symptoms, has been shown to reduce the catalepsy significantly (P<0.001) with Cyamopsis tetragonoloba methanol plant pod extract (200 and 400 mg/kg) doses. Tacrine-induced jaw movements and bursts symptoms closely resembling the PD symptoms shown to reduce treatment with plant extract and helped to manage the uncontrolled movements. The biochemical parameters like Lipid peroxidation (LPO), Glutathione (GSH), and Superoxide dismutase (SOD) were assessed using control, standard, and 200 and 400 mg/kg Cyamopsis tetragonoloba extract, results revealed that rats treated with plant extracts had increased levels of GSH and SOD which was found to be lower in the patients of PD and further plant extract analysis found efficient in reducing the LPO activity which helps to decrease...
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Posted by admin on May 31, 2021 in |
Covid-19 pandemic has enforced the entire scientific community to work together and find a solution for the adversity the whole world is facing. This has called for immediate actions, and the most common point of discussion and rapid way to tackle this is to repurpose the previously approved molecules and check their activity against this virus. The role of computational techniques has paved the way for rapid screening of molecules so as to provide us an insight on to designing drugs to inhibit this virus. Our group has screened the Dug bank database containing 8696 molecules. These molecules were screened using three tired molecular docking protocol. We utilized 5R82 as our target structure for the main protease enzyme of SARS-CoV-2, as it was the best available structure in Protein Data Bank. After screening the database, we obtained 200 molecules having docking scores better than the standard molecules (Ritonavir and Lopinavir). Eventually, after detailed analysis, we selected three molecules DB02307, DB04226, and DB01713, for Molecular dynamics simulation study and also...
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Posted by admin on May 31, 2021 in |
Tea tree oil, an essential oil, has claims for analgesic, antimicrobial, antiseptic, and anti-inflammatory activity. Its complex composition is governed by ISO 4730:2017. It is extensively used in skin and hair care cosmeceuticals, often in combination with neem seed oil. Neem seed oil has potent antimicrobial, skin rejuvenating, and conditioning action attributed to the presence of micro constituents and complex lipid composition of triglycerides, diglycerides, and monoglycerides of both saturated and unsaturated fatty acids. Although few methods are available for estimation of individual oils, simple spectrophotometric methods suitable for routine determination of either oil or for simultaneous estimation of both the oils are not reported. A novel, simple, validated UV spectrophotometric method for simultaneous estimation of tea tree oil and neem seed oil in bulk and cosmeceutical formulation has been developed after optimization and validation of UV spectrophotometric method for individual estimation of oils. The method relies on the measurement of absorbance at 267nm and 284nm, λmax of tea tree oil, and neem seed oil, respectively. Linearity is established...
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Posted by admin on May 31, 2021 in |
The purpose of this research was to obtain directly compressible agglomerates of simvastatin using a crystallo-co-agglomeration technique and determine its in-vivo performance in rats. Simvastatin Dicalcium phosphate co agglomerates were prepared from Dichloromethane: Isopropyl Myristate: Distilled water system containing polyvinyl pyrrolidone (PVP). Dichloromethane acted as a good solvent for simvastatin, the water acted as a bad solvent, and isopropyl myristate acted as a bridging liquid for agglomeration. 23 factorial design was used for optimization, wherein the factors were stirring speed, polymer concentration, and amount of bridging liquid. The responses evaluated were dissolution, b value, and Carr’s index. The agglomerates were characterized by powder x-ray diffraction (PXRD), which showed that there is a decrease in crystallinity or partial amorphization of the drug in its agglomerated form. Micromeritic and compression properties of the agglomerates were affected by an incorporated polymer. Dissolution of agglomerates (91.73%) increased as compared to a pure drug (25.96%). The agglomeration increased the flow property of Simvastatin which was indicated by Carr’s index of a drug (40.81) and an...
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Posted by admin on May 31, 2021 in |
The constant increase in the emergence of multidrug resistance among Staphylococcus aureus strains threatens public health. The study aimed at evaluating the anti-S. aureus activity, pharmacokinetic properties and interactions of compounds from Erianthemum dregei with proteins in S. aureus. Anti-S. aureus activity was investigated by broth dilution method while gas chromatography-mass spectrometry (GC-MS) was used to identify the compounds. The drug-likeness, pharmacokinetic and toxicity profiles of the compounds were predicted by SwissADME and PreADMET tools. AutoDock Vina was used to assessing the binding affinities of the docked ligand-receptor complexes. The extract revealed the minimum inhibitory concentration value of 0.78 mg/mL. Phytol (93.58%) and 3-tetradecyn-1-ol (6.42%) were the revealed constituents. In-silico predictions suggested both compounds to have drug-like properties as they adhered to the Lipinski’s rule of five. Phytol was found to have non-mutagenic effects, while 3-tetradecyn-1-ol was predicted to be mutagenic. The compounds were non-carcinogenic on mice model and carcinogenic on rat`s. Phytol has a binding affinity to DNA-gyrase and FtsZ with docking energy values of -4.1 and -5.3...
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