Posted by admin on Apr 30, 2021 in |
An alternative, economically viable, and laboratory-friendly synthetic method described for the preparation of Bimatoprost, an anti-glaucoma drug. Stereospecific synthesis of Bimatoprost has been achieved with commercially available (-) Corey lactone i.e., diol, as a starting material. To our delight, the replacement of the benzoyl (Bz) group with Tetrahydropyranyl ether (Thp) protection resulted in yield improvement. Tetrahydropyranyl ether (Thp) is notorious as a useful protecting group for alcohols in organic synthesis. It has numerous benefits, such as low cost, ease of insertion, general stability to most non-acidic conditions. It offers good solubility and easy to remove if the functional group it protects requires manipulation. Moreover, it described the successful application of luche’s reduction for the conversion of α, β-unsaturated ketone 8 to allylic alcohol 9 using CeCl3 7H2O, NaBH4. This lanthanide combination with borohydride enables the selective 1, 2-hydride attack on the carbonyl group, instead of an undesired 1, 4-hydride attack, which leads to the formation of desired allylic alcohol without disturbing the double...
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Posted by admin on Apr 30, 2021 in |
The aim of the present study is to apply the Design of Experiments (DoE) to develop an assay method for the separation of amino acids by RP-HPLC. The method used in this study is the DoE Combined- Randomized method. Design of Experiments allows interpreting the results with better outcomes and enhanced understanding process. Ion pair reagent- Octane 1 Sulphonic acid sodium salt monohydrate of 0.01M at pH 2.2 is used along with Methanol and Tetra-hydro Furan. The column used is INETSIL C8, with 5µ Particle size and 4.6×250 mm column dimensions. The Solvent composition and ion pair concentration is evaluated as variables in the DoE. The wavelength for all amino acids is 205 nm. Conclusively DoE is an efficient tool for the separation of amino acids. Method development was established, and the design is validated. The proposed method has adequate reproducibility and accuracy for the estimation of the amino acids in the routine...
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Posted by admin on Apr 30, 2021 in |
Angiogenesis is an inevitable process of tumour growth, invasion, and metastasis. Understanding the process of new blood vessel formation has gained prime importance recently, and anti-angiogenic therapy has now been considered as an important strategy for cancer therapy. Many bioactive compounds, especially of plant origin, have been explored for their anti-angiogenic potential. The present study was formulated to evaluate the effect of Majorana hortensis leaf extract on angiogenesis, in an effort to examine its influence against cancer cell proliferation, invasion, and metastasis. Cell viability analysis in non-transformed chick embryo fibro-blasts showed that Majorana hortensis leaf extract alone exhibited slight cytotoxicity as assessed by MTT and SRB assay. However, in combination with etoposide, the leaf extract exhibited a synergistic effect as evident from the improved cell viability in chick embryo fibroblasts. Using CAM assay, the anti-angiogenic potential of the Majorana hortensis leaf extract alone was inferred from the significant reduction in blood vessel formation. The anti-invasive potential of M. hortensis leaf extract was further substantiated by a scratch assay using...
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Posted by admin on Apr 30, 2021 in |
The biotransformation of different nitrile compounds into valuable acids was attempted using whole cells of nitrile hydrolyzing enzyme produced from a bacteria strain (BITSN007) isolated from sludge sample of Hot spring Surajkund, Jharkhand. Based on the biochemical characterization, the isolate producing nitrilase is predicted as Bacillus sp. The nitrilase production was observed as intracellular and inducible in nature. The preliminary results showed broad substrate specificity of enzyme, and the highest activity was obtained at 50 ºC and at pH 7.0 with a substrate concentration of 5 mM. Out of all the substrates, nitrilase showed greater activity for propionamide and converted it to propionic acid, which finds application in different food and chemical...
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Posted by admin on Apr 30, 2021 in |
The SARS-CoV-2 virus is the causative agent of the Covid-19 pandemic. The major proteins of this virus, like covid main protease RNA dependent RNA polymerase, are being extensively studied with the hopes of finding an effective molecule that would bind to these proteins and lead to their inhibition. The inhibition of these critical viral proteins would hamper SARS-CoV-2 infection. With most of the currently available drugs falling short of providing an absolute cure and the reported side-effects of the current medications, a lot of phytochemicals are being investigated for their efficacy against the viral proteins. In our preliminary in-silico analysis, we found that the Ocimum Sanctum (Holy Basil/Tulsi) flavonoids, orientin, and vicenin-1, showed energetically favoured docking with the functionally critical amino acid residues of covid main protease, RNA dependent RNA polymerase, Spike protein, nucleocapsid, and 3a proteins of SARS-CoV-2 virus. These results hold promise for orientin and vicenin-1 to be further investigated as potential therapeutic drugs against SARS-CoV-2...
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