Posted by admin on Jan 31, 2021 in |
The present UV Spectrophotometric and RP-HPLC method was simple, accurate, precise, specific, and sensitive. Spectrophotometric method was developed in water and very useful for analysis of bulk drug and injection formulation. RP-HPLC method was developed in Phosphate buffer (pH 3.0 adjusted with orthophosporic acid) and acetonitrile (70:30) using column HiQsil C18 column (250 × 4.6 mm; 5 μm). Flow Rate: 1.0 ml / min. The method was validated for the determination of granisetron hydrochloride in bulk and parenteral dosage form. The standard solution of granisetron hydrochloride in water showed maximum absorption at 301 nm with correlation, slope, and intercept 0.9998, 0.04198, and 0.01255, respectively and the percentage recovery of the formulation was 99.965% by UV spectrophotometry. By RP-HPLC, the % RSD of intraday and interday precision was 0.82% and 1.40 %, respectively. The % means recovery amount of Granisetron hydrochloride in 80 %, 100%, 120% was 94.3%, 94.9%, 95.2%, respectively, which reflect that the method was free from the interference of the impurities and other additives during the estimation...
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Posted by admin on Jan 31, 2021 in |
The present study was designed to evaluate the anticancer potential as well as cytotoxity of Chloroform extract of Thulasivettilacv of Piper betle L. is an evergreen perennial creeper belonging to family Piperaceae, and known to possess numerous medicinal properties. Current study focuses on evaluating antiproliferative potentialities of Betel leaves. The antiproliferative effect was tested against two cancer cell lines HeLa and HT 29, while cytotoxity was tested against in a normal myoblast cell line, L6 by MTT assay. Five different concentration of the chloroform extract were used for this study. Qualitative phytochemical analysis of leaf extracts showed the presence of tannins, alkaloids, phenols, flavonoids and glycosides. HeLa cell lines exhibited less antiproliferative activity 25.8 µg/ml ± 0.05% viability at 100µg/ml of extract by MTT assay and the IC50 value was found to be 45.19 µg/ml. In case of HT 29 colon cancer cell line, chloroform extract of plant showed 30.32 ± 0.04% µg/ml viability compared with the L6 normal cell line was24.00 ± 0.08% µg/ml. The IC50 values were...
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Posted by admin on Jan 31, 2021 in |
Fatty acid vesicles are novel vesicles incorporating fatty acids which enhance the brain targeting efficiency when compared to conventional liposomes. This study’s objective was to prepare, characterize, and evaluate fatty acid vesicles for brain targeting of a water-soluble drug, atomoxetine HCl through nasal route. The atomoxetine HCl loaded fatty acid vesicles were prepared by using the reverse phase evaporation method using Phospholipon 90 H, oleic acid, and ethanol. Prepared formulations were evaluated for parameters like particle size (152.9 nm), drug entrapment (90.4%), zeta potential (-9.41 mv), and in-vitro release was found to be 98% up to 4 h. SEM results showed spherical shaped vesicles. The ability of the prepared atomoxetine HCl loaded fatty acid vesicles to deliver atomoxetine HCl through the nasal route was studied by ex-vivo studies using goat nasal mucosa, which showed sustained release of 51% of the drug up to 4...
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Posted by admin on Jan 31, 2021 in |
Ethyl4-(2-hydroxy-benzylideneamino) benzoate Schiff base (C16H15NO3), were synthesized and the structure was studied against the bases of X-ray, UV, visible, elemental analysis, 1HNMR, IR and Mass spectroscopy. The crystal structure of the nickel complex has been determined by single crystal X-ray conformed the molecule, which indicate the compound is crystalline in the monoclinic C2 / c with a = 16.0916 (5) Å, b = 6.0315 (2) Å, c =29.0072 (10) Å, α = 90.00°, β= 101.856 (2)°, γ = 90.00°, V= 2755.3 (2) Å3 and Z = 8. Because of intra-molecular hydrogen bond involving the O atom of hydroxy group and N atom of azomethine group; the two benzene rings and azomethine group are practically coplanar. The Cobalt complex was prepared; the structure was characterized in the bases of IR spectra, elemental analysis, UV – visible, and conductance measurements. Also, the biological activity of both Schiff base and its Cobalt complex show that they are biologically...
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Posted by admin on Jan 31, 2021 in |
A simple, reproducible, and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Lornoxicam and Thiocolchicoside in pure form and marketed combined pharmaceutical dosage forms. A column having Symmetry (C18) (150mm × 4.6mm, 5µm) in isocratic mode with mobile phase containing Methanol: Phosphate Buffer (pH-3.8) (28:72 v/v) was used. The flow rate was 1.0 ml/min, and the effluent was monitored at 252 nm. The retention time (min) and linearity range (ppm) for Lornoxicam and Thiocolchicoside were (1.794, 3.440 min) and (10-30, 10-50), respectively. The method has been validated for linearity, accuracy, and precision, robustness, and limit of detection, and limit of quantitation. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.86µg/ml and 2.58µg/ml for Lornoxicam and 1.28µg/ml 3.84µg/ml for Thiocolchicoside respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of Lornoxicam and Thiocolchicoside in...
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