Posted by admin on Feb 28, 2021 in |
DNA bar-coding is an ordered strategy that utilizes a little genetic marker in insect DNA to distinguish animal categories, as well as unidentified species. The present investigation manages the utilization of COI gene sequences in the distinguishing proof and documentation of edible insects in Nagaland. More than 2000 types of edible insects are recorded in more than 300 ethnic groups all around the world. It is evaluated that more than 200 types of species of edible insects and other invertebrates are consumed in North East India. In the preliminary study, Vespa affinis was selected and morphologically identified with the help of local tribals in Nagaland. Vespa affinis was identified by using phylogenetic tree analysis, and COI gene sequences were submitted in NCBI to received the accession number (MN820554), BOLD (ACH6486), and COI gene sequence were submitted in BOLD to generated DNA barcode for edible insects of Vespa...
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Posted by admin on Feb 28, 2021 in |
The contamination of Rice and its by-products with storage fungi, especially toxigenic Aspergillus flavus and Aflatoxin B1 leads to potential health hazard to human and domestic animals. Therefore, the present investigation was focussed on the comparative analysis of the fungal contamination with A. flavus and Aflatoxin B1 in Raw and Parboiled paddy and its milling fractions like Husk, Brow Rice, Polished rice and rice bran in a normal rice mill and controlled mill environment. The results of the study indicated that the raw and parboiled paddy showed 100% contamination with fungi in untreated grains in which A. flavus occurred 63% and 77%, respectively. The level of Aflatoxin B1 in untreated Raw and Parboiled paddy was 36 ppb and 50 ppb, respectively. The husk analyzed for the presence of fungal contamination showed 100%, 85%, and the A. flavus was 43%, 30% respectively for Raw and Parboiled grains. But there is no Aflatoxin B1. Found in either of the husk samples. The brown rice exhibited almost closer to the untreated Raw...
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Posted by admin on Feb 28, 2021 in |
Tuberculosis (TB) is one of the leading infectious diseases and health burdens in the world. One-third of the world’s population, including 40% from India, is estimated to be infected with tuberculosis. Current studies have indicated the urgent need for the development of new, safe, and efficacious drugs to help reduce the global burden of tuberculosis. Novel antimycobacterial scaffolds from natural products have recently been reported. Natural products of plant biodiversity have received considerable attention as potential anti-TB agents since they are a proven template for the development of new molecules against tuberculosis. We have selected eleven medicinal plants on the basis of four criteria to choose a plant for antituberculosis activity. The plants were having antituberculosis effect, hepatoprotective effect, immune-modulatory action, and having the ability to enhance bioavailability. All selected eleven plants were reviewed in ancient literature and research article-based review. So here, our objective is to study pharmacognostic, physochemical and cytotoxicity screening of eleven selected medicinal plants. There are three different extracts of eleven selected medicinal plants that...
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Posted by admin on Feb 28, 2021 in |
Drynaria quercifolia rhizome (DQR) ethnomedicinal information shows the traditional usage of this rhizome for the treatment of body and joint pain, diarrhoea, typhoid, cholera, and skin diseases. The fresh juice of rhizome is still being consumed as an energy drink by tribes and localities of Tamil Nadu and Kerala. However, no investigations have been carried out to claim the anti-arthritic activity of DQR to date. In-vitro studies on anti-arthritic effect of the crude extract were performed. In-vitro anti-arthritic activities were evaluated by using elastase, PLA2, hyaluronidase, and proteinase and assays like protein denaturation inhibition, membrane lysis, and NO scavenging activity. Based on the promising in-vitro results, the use of DQR as an anti-arthritic agent may be agreed upon, and further establishment towards the safety and efficacy is conceded. Acute toxicity study revealed NOAEL (No Observed Adverse Effect Level) up to 2000 mg/kg body weight. Oral administration of 1000 mg/kg body weight (crude extract) for 14 days led to the well tolerance level in albino...
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Posted by admin on Feb 28, 2021 in |
Objective: Stability of In-vitro Diagnostics (IVDs) reagents was performed to check its quality standards, performance, and linearity. IVDs like Calcium reagent containing Arsenazo III were studied by Accelerated stability studies by considering temperature, pH, and light parameters. Materials and Methods: Stability data were obtained by using different instruments like UV spectrophotometer, Biochemistry analyzer, pH meter, HPLC, and HPTLC. This gives information about the degradation that occurred during storage, transportation, etc. Results: Calcium reagent containing Arsenazo III degrade 90.81% when placed at 42 °C by UV spectrophotometer analysis. The stability conditions’ effect on actual serum concentration was measured by taking reagent performance on a biochemistry analyzer. The linearity of reagents decreases at 42 °C and at normal temperature, linearity does not change. HPLC spectra gave degradation of reagent, which was analyzed by its retention time, peak height, and % area. Arsenazo III produces 91.25% remains undecomposed in 3 months when exposed to light. The shelf life of the calcium reagent was found to be 85.36. HPTLC spectra gave degradation of...
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