Posted by admin on Feb 28, 2021 in |
The novel human coronavirus which has been designated as SARS-CoV2 initially appeared in December 2019 in Wuhan, China causing a respiratory illness called COVID-19. The SARS-CoV2 is a β coronavirus belonging to the family Coronaviridae is a global public health emergency infecting many people all around the world, especially in India with more than 10.8 million cases. Henceforth there is an urgent requirement for a novel drug that counters SARS-CoV2. The present study was intended to evaluate the therapeutic potential of natural products in plants as a potential inhibitor of SARS-CoV2 Mpro (6Y2F, 6Y2G, 6YB7). Molecular docking was performed by Autodock version 4.2, by means of the Lamarckian Genetic Algorithm, to evaluate the possibility of docking. SARS-CoV2 Mpro was docked with twenty-two compounds namely Apigenin, Coriandrin, Curcumin, Catechin, Quercetin, Oleanolic acid, Rosmarinic acid, Ursolic acid, Glucobrassicin, Kaempferol, Gingerol, Naringenin, Carvacrol, Limonene, Eucalyptol, Berberine, Luteolin, Gallic acid, Gedunin, and Nimocinol, and docking was analyzed by Autodock 4.2 and Pymol. HIV drugs, Nelfinavir and Saquinavir, were used as standards for comparison....
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Posted by admin on Feb 28, 2021 in |
Deracoxib is a coxib class of Non Steroidal Anti-Inflammatory Drug (NSAID). Like other NSAIDs, its effects are caused by inhibition of Cyclooxygenase (COX) enzymes. Deracoxib causes greater inhibition of COX-2 than of COX-1. The aim of this proposed method was to develop and validate the UV spectrophotometric method for the routine quality control check of API containing 25 mg Deracoxib intablets dosage form. To investigate the most preferable dissolution method includes dissolution media as pH 6.8 sodium phosphate buffer + 1 % Sodium Lauryl Sulphate, 900ml, temperature as 37±0.5°C, RPM as 75, time 45 min. The absorbance maximum of Deracoxib was found to be 255nm. The concentration range of the proposed method was 5-15µg/ml and linearity as r2 = 0.9959.The result of intraday &interday precision was 0.1458% and 0.1214% RSD respectively. The results of the percentage recoveries were 99.2%, 99.3%, and 99.1%. The effective dissolution method was developed and validated by UV spectrophotometer which used to be more applicable in various pharmaceutical...
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Posted by admin on Feb 28, 2021 in |
The present study was aimed to formulate and evaluate floating microspheres of Baclofen. The research work’s objective was to retain Baclofen in the stomach for a prolonged period of time, which has absorption window in the upper gastrointestinal tract. The microspheres were prepared by solvent evaporation technique. A 32 full factorial was applied to investigate the combined effect of the two independent variables, i.e., the concentration of Eudragit RL100 (X1) and concentration of Eudragit RS 100 (X2) on the dependent variables particle size (Y1), percentage drug entrapment efficiency (Y2), percentage buoyancy (Y3), in-vitro drug release at 1 h (Y4), in-vitro drug release at 6 h (Y5). Results of the multiple regression analysis revealed that in-vitro drug release decreased and particle size, percentage drug entrapment efficiency, percentage buoyancy was increased with increasing the concentration of Eudragit RL100 and Eudragit RS100. The optimized formulation has a particle size of 115.96 µm, percentage drug entrapment of 90.06%, and buoyancy of 90.76%. In-vitro drug release of Baclofen floating microspheres showed a sustained release...
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Posted by admin on Feb 28, 2021 in |
The protein MTA1 with histone deacetylase activity is a member of the nucleosome remodeling complex encoded by the mta1 gene is frequently overexpressed in biologically aggressive epithelial neoplasms. MTA1 is a nuclear co-regulatory molecule that is highly expressed in many cancers and correlates with tumor metastasis and progression. MTA1 alters signaling events in the tumor micro-environment. MTA1 contributes to cancer progression, may serve as a biomarker and therapeutic target for cancer. The main objective of this study is to perform in–silico docking studies and energy minimizations for active sites of mutated MTA1 protein by selecting different naturally available antioxidant molecules in foods to facilitate ligand-binding site interactions and identify the best from the selected...
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Posted by admin on Feb 28, 2021 in |
Staphylococcus aureus is a frequent colonizer of the human body as well as a serious human pathogen. It can cope with stress factors and acquire resistance to antibiotics, thus rendering treatment difficult. Discovering new treatments for this disease is urgently required, especially in view of the emergence of multiple drug resistant organisms and to reduce the total duration of current treatments. Exfoliative toxin A (ETA), produced by Staphylococcus aureus causes scalded skin syndrome which is characterized by the separation of layers of skin. It affects newborns and adults primarily. Hence, therapies for Staphylococcal scalded skin syndrome (SSSS) are critically needed. Seeking a more effective compound for the treatment has been one of the great interests. In the present study, compounds from different plant extracts are permissible to interact with ETA using Schrodinger. The best effective compound gymnemic triacetate was selected from the list of different compounds based on its docking score and binding energy. The result showed that the herbal compound gymnemic triacetate possesses anti-bacterial activity by blocking exfoliative...
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